Protection AgainstSalmonellaTyphimurium,SalmonellaGallinarum, andSalmonellaEnteritidis Infection in Layer Chickens Conferred by a Live AttenuatedSalmonellaTyphimurium Strain

Lee, John Hwa

doi:10.4110/in.2015.15.1.27

Cited by 13 publications

(7 citation statements)

References 45 publications

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“…CS(OMP+FLA)-based vaccines induced higher antibody and/or cellular immune responses, correlated with improved protective efficiency, but the commercial vaccine did not induce any such substantial immune response, suggesting the need for further studies to understand its mechanism of induction of protection in broilers. However, the commercial live vaccine stimulated significant levels of specific IgG and IgA responses in layer birds [ 41 ]. This may be due to the age difference between broilers and layers at the time of vaccination.…”

Section: Discussionmentioning

confidence: 99%

Mannose-Modified Chitosan-Nanoparticle-Based Salmonella Subunit OralVaccine-Induced Immune Response and Efficacy in a Challenge Trial in Broilers

et al. 2020

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Controlling Salmonella enterica serovar Enteritidis (SE) infection in broilers is a huge challenge. In this study, our objective was to improve the efficacy of a chitosan nanoparticle (CS)-based Salmonella subunit vaccine for SE, containing immunogenic outer membrane proteins (OMP) and flagellin (FLA), called the CS(OMP+FLA) vaccine, by surface conjugating it with mannose to target dendritic cells, and comparing the immune responses and efficacy with a commercial live Salmonella vaccine in broilers. The CS(OMP+FLA)-based vaccines were administered orally at age 3 days and as a booster dose after three weeks, and the broilers were challenged with SE at 5 weeks of age. Birds were sacrificed 10 days post-challenge and it was observed that CS(OMP+FLA) vaccine surface conjugated with both mannose and FLA produced the greatest SE reduction, by over 1 log10 colony forming unit per gram of the cecal content, which was comparable to a commercial live vaccine. Immunologically, specific mucosal antibody responses were enhanced by FLA-surface-coated CS(OMP+FLA) vaccine, and mannose-bound CS(OMP+FLA) improved the cellular immune response. In addition, increased mRNA expression of Toll-like receptors and cytokine was observed in CS(OMP+FLA)-based-vaccinated birds. The commercial live vaccine failed to induce any such substantial immune response, except that they had a slightly improved T helper cell frequency. Our data suggest that FLA-coated and mannose-modified CS(OMP+FLA) vaccine induced robust innate and adaptive cell-mediated immune responses and substantially reduced the Salmonella load in the intestines of broilers.

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Section: Discussionmentioning

confidence: 99%

Mannose-Modified Chitosan-Nanoparticle-Based Salmonella Subunit OralVaccine-Induced Immune Response and Efficacy in a Challenge Trial in Broilers

et al. 2020

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“…Previously, live Salmonella vaccines were reported to induce cross-immunity against related serovars (MATULOVA et al, 2013;MOHLER et al, 2008). Although ST infection is common in chickens, it does not cause severe disease in poultry (CHAPPELL et al, 2009); consequently, attenuated ST strains have been used successfully to express foreign antigens and have been suggested as safe strains (LEE, 2015). In this study, administering a ST vaccine did not induce mortality or any adverse effects in the vaccinated birds.…”

Section: Discussionmentioning

confidence: 49%

“…In recent years, the use of genetically modified S. Typhimurium (ST) strains as immunization agents has gained remarkable popularity, since these strains have no clinical side effects (CHAUDHARI; LEE, 2013) and may provide some protection against other serovars (LEE, 2015). According with HUANG et al, (2016), an attenuated ST vaccine strain has shown to offer protection against S. Enteritidis virulent challenge in mice.…”

Section: Introductionmentioning

confidence: 99%

Salmonella Typhimurium Vaccine to Control a Brazilian Salmonella Heidelberg Strain in Broiler Chickens

Hayashi¹,

Tujimoto-Silva²,

Muñíz³

et al. 2018

Ars Vet

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Foodborne Salmonella infections in humans, which results from the consumption of contaminated poultry meat and eggs, are a major public health concern. Vaccination of animals against Salmonella is one strategy to prevent these infections and reduce the risks to public health. Live attenuated Salmonella enterica vaccines can confer protection against salmonellosis by inducing both cell-mediated and mucosal immune responses. This study assessed a live, attenuated Salmonella enterica Typhimurium (ST) vaccine in broiler chickens against a heterologous challenge with Salmonella Heidelberg (SH) by evaluating bacterial quantification, immune cells infiltration, and cytokine gene expression in the cecum. The treatments were: T1, non-vaccinated, non-challenged; T2, non-vaccinated, SH-challenged; T3, ST-vaccinated and SH-challenged. At 28 days of age, the ST-vaccinated group had significantly recovered reduction of SH in the crop (P<0,01) and cecum (P = 0,021) compared to the non-vaccinated SH-challenged group, with no significant changes (P˃0,05) in macrophages, T CD4+, or T CD8+ cells dynamics during the same period. Aerosol vaccination on the first day promoted greater interleukin-12 expression in the liver (P<0,05) and interleukin-10 expression and T CD8+ cells in the ileum 16 hours after housing. After prime-boosted oral immunization on the 13th day, the vaccinated group had greater expression of macrophages and T CD4+ cells in the liver (P<0,05) than the control group. Two doses of a live ST-attenuated vaccine promoted a partial cross-protective effect against SH strain UFPR1 challenge in broilers.

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“…Salmonella vaccines can also offer inter-serotypic cross immunity. For example, a study showed that layer chickens are protected against Salmonella typhimurium , S. gallinarum , and S. enteritidis infections when given a live attenuated Salmonella enterica serovar typhimurium strain at 7 days of age followed by boosting at 5 weeks of age [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

“…Variable morbidity and mortality rates in chickens infected with S. gallinarum have been reported in earlier studies depending on the age and immune status of the birds, virulence of the bacterial strains, management status, rearing system, biosecurity measures, etc. For example, the S. gallinarum produces very high mortality (40%–100%) in chicks with severe inflammation in the liver and spleen [ 29 , 31 , 32 ]. However, the mortality rates of S. gallinarum infection in layer chickens varied greatly between studies.…”

Section: Discussionmentioning

confidence: 99%

Fowl typhoid live lyophilized vaccine applied at 3-month intervals protected layer chickens from Salmonella gallinarum infection and prevented cloacal shedding

Akter¹,

Nooruzzaman²,

Belal³

et al. 2022

J Adv Vet Anim Res

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Objective: Here, we developed and tested the efficacy of a vaccination protocol based on a commercially available live attenuated Salmonella enterica serovar Gallinarum (Salmonella galli¬narum) in layer chickens. Materials and Methods: 50 layer chickens of 16 weeks age were obtained and divided into two groups (n = 25), control and vaccinated. The vaccinated group received Salmonella vaccine at 0.2 ml/bird, s/c route at 16, 18, 30, and 42 weeks of age. At 21 weeks of age, birds from both groups were challenged with S. gallinarum orally at 4 × 107 colony-forming unit per bird. Results: Both rapid serum plate agglutination and enzyme-linked immunosorbent assay demonstrated a rising rate of seroconversion in vaccinated birds across the study period, with a 4% positive rate at 18 weeks, 56% at 21 weeks, 60% at 30 weeks, and 64% at each time point of 42 and 54 weeks. The vaccine showed 100% clinical protection and reduced the Salmonella shedding in the feces and eggs of the challenged birds. On the contrary, the unvaccinated challenged birds showed clinical signs and lesions typical of Salmonella infections with morbidity and mortality rates of 36% and 20%, respectively, and had high rates of Salmonella shedding in feces and eggs. Conclusions: With the proposed vaccination schedule Salmonella shedding was prevented, and a high seroconversion was confirmed. To prevent Salmonella infections in laying flocks, a 3-month interval immunization program is advised starting at the pre-laying stage.

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Protection AgainstSalmonellaTyphimurium,SalmonellaGallinarum, andSalmonellaEnteritidis Infection in Layer Chickens Conferred by a Live AttenuatedSalmonellaTyphimurium Strain

Cited by 13 publications

References 45 publications

Mannose-Modified Chitosan-Nanoparticle-Based Salmonella Subunit OralVaccine-Induced Immune Response and Efficacy in a Challenge Trial in Broilers

Mannose-Modified Chitosan-Nanoparticle-Based Salmonella Subunit OralVaccine-Induced Immune Response and Efficacy in a Challenge Trial in Broilers

Salmonella Typhimurium Vaccine to Control a Brazilian Salmonella Heidelberg Strain in Broiler Chickens

Fowl typhoid live lyophilized vaccine applied at 3-month intervals protected layer chickens from Salmonella gallinarum infection and prevented cloacal shedding

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