Protection by Pyruvate Infusion in a Rat Model of Severe Intestinal Ischemia-Reperfusion Injury

Petrat, Frank; Rönn, Thomas; Groot, Herbert de

doi:10.1016/j.jss.2009.12.005

Cited by 41 publications

(31 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, pyruvate infusion has been reported to have a local protective effect against I/R injury in Table 1. The MDA and GSA levels of study groups rat small intestine (13). It has been shown in a rat model that proanthocyanidin has a protective effect against mesenteric I/R injury both at intestinal and distant organ level (14).…”

Section: Discussionmentioning

confidence: 98%

The Effects of Apelin on Mesenteric Ischemia and Reperfusion Damage in an Experimental Rat Mode

Sagiroglu¹,

Oguz²,

Sagiroglu³

et al. 2012

Balkan Med J

View full text Add to dashboard Cite

Objective: Intestinal ischemia-reperfusion (I/R) injury is associated with high morbidity and mortality rates. There is ongoing research to find an effective preventive or treatment agent. We aimed to evaluate the effects of apelin 13 (AP) on intestinal I/R injury in a rat model. Material and Methods:Twenty-four male Sprague-Dawley rats aged 6-8 weeks and weighing 280±20 g were equally divided into three groups (control, I/R and I/R+AP). The control group underwent superior mesenteric artery (SMA) mobilization alone without any clamping. In the I/R and I/R+AP groups, an atraumatic microvascular bulldog clamp was placed across the SMA at its point of origin from the aorta. In the I/R+AP group, 2 µg/kg/d apelin was administered intraperitoneally. After 60 minutes of ischemia, relaparotomy was performed to remove the microvascular clamp on the SMA for 3 hours of reperfusion. After 3 hours, tissue samples were obtained for biochemical [malondialdehyde (MDA) and glutathione (GSH) levels] and histopathological analyses.Results: MDA levels were significantly higher in the I/R group compared to the control group. Although MDA levels were lower in the I/R+AP group compared tothe I/R group, the difference was not statistically significant. There was also no significant difference between the I/R+AP and I/R groups regarding GSH levels. The median histopathological grade was significantly lower in the I/R+AP group compared to the I/R group (p=0.001).Conclusion: Apelin appeared to have a positive effect on oxidative injury; this did not reach statistical significance. Thus, the role of apelin and associated findings in the initial treatment of intestinal ischemia needs further large-scale animal studies before human use.

show abstract

Section: Discussionmentioning

confidence: 98%

The Effects of Apelin on Mesenteric Ischemia and Reperfusion Damage in an Experimental Rat Mode

Sagiroglu¹,

Oguz²,

Sagiroglu³

et al. 2012

Balkan Med J

View full text Add to dashboard Cite

show abstract

“…Rats were anesthetized with isoflurane, treated with ketamine/lidocaine for analgesia and subjected to surgical procedures as described previously [7,24]. Briefly, after placement of catheters within the left carotid artery and the right jugular vein, respectively, the arteria mesenterica superior was occluded using an atraumatic mini-bulldog.…”

Section: Methodsmentioning

confidence: 99%

“…Subsequent to the determination of the macroscopic score, each 10-cm segment was homogenized, the homogenates were centrifuged, and the resulting supernatants pooled and immediately used for determination of hemoglobin content and myeloperoxidase activity [24]. The hemoglobin content of the small intestine was determined from the absorption of the hemoglobin Soret band [24] and the activity of myeloperoxidase from the H 2 O 2 -dependent oxidation of o -dianisidine [24,25].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Protection from Glycine at Low Doses in Ischemia-Reperfusion Injury of the Rat Small Intestine

Petrat¹,

Drowatzky²,

Boengler³

et al. 2011

Eur Surg Res

View full text Add to dashboard Cite

Background: Glycine at high doses is known to protect the small intestine against ischemia-reperfusion (I/R) injury. Here, we studied whether glycine at low clinically applicable doses has a protective effect. Methods: In series 1, intestinal I/R was induced in male Wistar rats by occlusion (90 min)/reopening (120 min) of the superior mesenteric artery. Glycine was intravenously infused for 30 min before ischemia (pre-ischemic infusion), and once again from 30 min before until 60 min after reperfusion. Total glycine doses applied over the 120-min infusion were 5, 10, 20, and 75 mg glycine/kg. In series 2, pre-ischemic blood plasma glycine concentrations were determined under the conditions of series 1. Results: In series 1, attenuation of I/R injury was comparable at 10, 20, and 75 mg glycine/kg, but less at 5 mg/kg (as indicated by less intestinal hemorrhages and better preserved mean arterial blood pressure, among other signs). In series 2, pre-ischemic blood plasma glycine concentrations increased with increasing glycine doses from 280 to 330, 340, 380, and 680 µM, respectively. Conclusion: These results demonstrate that even at a dose 50 times lower than previously applied – and at only slightly elevated plasma concentrations – glycine provides full protection against I/R injury of the small intestine.

show abstract

“…Arthurs et al (2011) challenged this philosophy by performing laparotomy only on patients who had signs of peritoneal inflammation or deteriorated clinically following initial revascularisation. Over 30% of patients in the endovascular therapy group did not ultimately require laparotomy, thereby avoiding further physiologic insult to patients who are already critically ill. Another important issue is to what extent ischaemia-reperfusion injury of the intestine, leading to microvascular injury and cellular necrosis and apoptosis, contributes to morbidity and mortality in patients in whom arterial revascularisation is attained and whether various recent advances in preventing or limiting this phenomenon described in the experimental situation can be translated clinically (Santora et al, 2011;Petrat & de Groot, 2011;Flessas et al, 2011).…”

Section: Acute Mesenteric Ischemiamentioning

confidence: 99%

Percutaneous Angioplasty and Stenting for Mesenteric Ischaemia

He¹,

Riordan²

2012

Angioplasty, Various Techniques and Challenges in Treatment of Congenital and Acquired Vascular Stenoses

View full text Add to dashboard Cite

Protection by Pyruvate Infusion in a Rat Model of Severe Intestinal Ischemia-Reperfusion Injury

Cited by 41 publications

References 23 publications

The Effects of Apelin on Mesenteric Ischemia and Reperfusion Damage in an Experimental Rat Mode

The Effects of Apelin on Mesenteric Ischemia and Reperfusion Damage in an Experimental Rat Mode

Protection from Glycine at Low Doses in Ischemia-Reperfusion Injury of the Rat Small Intestine

Percutaneous Angioplasty and Stenting for Mesenteric Ischaemia

Contact Info

Product

Resources

About