Protection conferred by Trypanosoma evansi infection against homologous and heterologous trypanosome challenge in rabbits

Uche, U.E.; Jones, T.W.

doi:10.1016/0304-4017(94)90032-9

Cited by 11 publications

(9 citation statements)

References 15 publications

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“…Besides being revealed by antibodies from all experimental animals, the 66 kDa polypeptide was also recognized by sera of all naturally infected horses and dogs and of most free-ranging coatis. A polypeptide with similar mass (67 kDa) previously identified as a surface antigen of T. evansi (UCHE; ROSS; JONES, 1992) was also consistently recognized by rabbits experimentally infected (UCHE; JONES; BOID, 1993) and showed to be associated with protection (UCHE et al, 1994). The 66 kDa polypeptide detected in our experiment may correspond to the 64 kDa polypeptide recognized in a Venezuelan stock of the parasite by sera of experimentally infected horses (UZCANGA et al, 2002).…”

Section: Discussionsupporting

confidence: 79%

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Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Aquino¹,

Machado²,

Lemos³

et al. 2010

Rev. Bras. Parasitol. Vet. (Online)

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The present research investigated the presence of T. evansi antibodies in animals from the subregion of Nhecolandia, in the Pantanal Sul-mato-grossense, by means of an enzyme linked immunosorbent assay (ELISA) and indirect immunofluorescence antibody test (IFAT), and the pattern of polypeptide recognition by sera from experimentally and naturally infected hosts using Western blotting. Serum samples were obtained from bovines (n = 102), horses (n = 98), and dogs (n = 55), and from 32 free-ranging coatis (Nasua nasua). None of the bovines were found positive, while sera from 16 dogs (29%) and 23 horses (23.4%) were positive by ELISA. Sera from 8 coatis (25%) were found positive using IFAT. Western blotting revealed major polypeptides of T. evansi with molecular weight ranging from 74 to 38 kDa. The polypeptides of 66, 48-46, and 38 kDa were identified by sera from experimentally infected bovines, donkeys, dogs, and coatis. The 48-46 and 38 kDa bands were mainly recognized in chronic phase of infection. The antigen with apparent molecular weight of 66 kDa, revealed by antibodies from all experimental animals, was also recognized in sera of horses and dogs from the Pantanal. The 48-46 kDa polypeptide was identified by antibodies from all naturally infected animals and must be further evaluated for use in specific diagnosis of T. evansi infection.Keywords: Trypanosoma evansi, ELISA-test, IFAT, Pantanal mato-grossense, antigenic characterization. ResumoO trabalho de pesquisa investigou a presença de anticorpos anti-T. evansi em animais da sub-região da Nhecolândia, no Pantanal sul-mato-grossense, pelo ensaio imunoenzimático (ELISA) e a reação de imunofluorescência indireta (RIFI). O reconhecimento de polipeptídeos de T. evansi foi realizado pela técnica de "Western blotting", utilizando soros de animais experimentalmente e naturalmente infectados. As amostras de soro foram obtidas de bovinos (n = 102), cavalos (n = 98) e cães (n = 55) e de 32 quatis de vida livre (Nasua nasua) do pantanal mato-grossense. Todos os soros dos bovinos foram negativos, enquanto soros de 16 cães (29%) e 23 cavalos (23,4%) foram positivos pelo ELISA. Soros de oito quatis (25%) foram positivos pela RIFI. Pelo "Western-blotting" foi possível revelar polipeptídeos de T. evansi, com peso molecular variando de 74 a 38 kDa. Os polipeptídeos de 66, 48-46 e 38 kDa foram identificados por soros de bovinos, cavalos, cães e quatis experimentalmente infectados. As bandas de 48-46 e 38 kDa foram reconhecidas principalmente na fase crônica da infecção. O antígeno com peso molecular aparente de 66 kDa, revelado por anticorpos de todos os animais experimentais, também foi reconhecido por soros de cavalos e cães do Pantanal. O polipeptídeo de 48-46 kDa foi identificado por anticorpos de todos os animais naturalmente infectados, devendo ser avaliado para o diagnóstico específico da infecção pelo T. evansi.

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Section: Discussionsupporting

confidence: 79%

“…Few studies on antigenic characterization of T. evansi have been undertaken (UCHE; JONES; BOID, 1993;JONES, 1994;QUEIROZ et al, 2001) and little is known so far regarding the polypeptide recognition patterns by antibodies from different host species.…”

Section: Introductionmentioning

confidence: 99%

Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Aquino¹,

Machado²,

Lemos³

et al. 2010

Rev. Bras. Parasitol. Vet. (Online)

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“…It already was described for T. evansi and other salivarian trypanosomatids in other hosts, that the humoral immune response, mainly IgM, plays an important role in the control of the circulating parasites (Roelants & Pinder 1984, Uche & Jones 1994. In our experiments, this correlation was not so clear since the infected rats displayed comparable antibody titers.…”

Section: Discussioncontrasting

confidence: 48%

“…Probably there exist characteristic mechanisms for controlling the parasitaemia in each animal species. Uche and Jones (1994) described that rabbits recognized a smaller spectrum of antigens than rats, reinforcing the idea of characteristic control mechanisms for T. evansi infection of each animal species. These peculiarities could be at least one of the reasons for the distinct aspects of the disease.…”

Section: Discussionmentioning

confidence: 68%

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Specific antibody levels and antigenic recognition of Wistar rats inoculated with distinct isolates of Trypanosoma evansi

Queiroz

Legey

Xavier

et al. 2001

Mem. Inst. Oswaldo Cruz

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Histopathology of Trypanosoma (Trypanozoon) evansi Infection in Bandicoot Rat. I. Visceral Organs

Biswas

Choudhury

Misra

2001

Experimental Parasitology

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Protection conferred by Trypanosoma evansi infection against homologous and heterologous trypanosome challenge in rabbits

Cited by 11 publications

References 15 publications

Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Specific antibody levels and antigenic recognition of Wistar rats inoculated with distinct isolates of Trypanosoma evansi

Histopathology of Trypanosoma (Trypanozoon) evansi Infection in Bandicoot Rat. I. Visceral Organs

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