Protection from infection with influenza A H7N9 virus in a mouse model by equine neutralizing F(ab′)2

Zhao, Zhongpeng; Fan, Cheng; Duan, Yueqiang; Zhang, Liangyan; Li, Min; Yang, Xiaolan; Li, Ruisheng; Yang, Peng; Wang, Xiliang

doi:10.1016/j.intimp.2014.08.019

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…Antibodies against FPV (named GX01) in horse serum were tested by HI according to a previously described method [12]. HI was assessed by using 50 μl of horse sera for a series 2× folded dilution and by 50 μl of 8HA units of the GX01 stain.…”

Section: Detection Of Hi Titre Of Horse Serum and Extraction Of Iggmentioning

confidence: 99%

Prophylactic Efficacy of Equine Immunoglobulin F(ab′)2 Fragments Against Feline Parvovirus

Liu

Zhang

Bai

et al. 2021

Appl Biochem Biotechnol

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Feline parvovirus (FPV), a type of parvovirus prevalent worldwide, can cause foetal death and acute enteritis in adult cats with severe leukopenia, and yet there are no effective drugs to prevent or treat FPV. Here, the immune effects of two FPV vaccines on horses were compared. IgG was extracted from FPV-immunized horse sera. Equine F(ab′) 2 fragments were obtained from pepsin-digested IgG and then purified by protein-G column chromatography. The results showed that the inactivated FPV oil vaccine was more effective than the inactivated FPV propolis vaccine in helping healthy horses to produce hyper-immune serum. Four methods were tested, among which the optimized octanoic acid-ammonium sulphate precipitation method was proved to be the best process for extracting IgG. The optimal condition for preparing F(ab′) 2 by pepsin digestion was 30°C for 3.5 h, and the content, purity and recovery of F(ab′) 2 were 8.64 mg/mL, 90.36% and 93.24%, respectively. Our equine immunoglobulin F(ab′) 2 fragments effectively neutralized activity in vitro against FPV, alleviated the clinical symptoms of FPVinfected cats, reduced the viral loads in the intestine and had prophylactic effects in FPV-infected cats. These results indicate that the F(ab′) 2 fragment prepared from inactivated FPV-immunized horses may be used as a prophylactic agent for diseases caused by FPV.

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Section: Detection Of Hi Titre Of Horse Serum and Extraction Of Iggmentioning

confidence: 99%

Prophylactic Efficacy of Equine Immunoglobulin F(ab′)2 Fragments Against Feline Parvovirus

Liu

Zhang

Bai

et al. 2021

Appl Biochem Biotechnol

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“…Therefore, much work will be needed to develop an effective ricin-specific antidote. Using our equine immunoglobulin F(ab′) 2 fragment preparation and evaluation technology [16,17], we developed biological products associated with ricin, and assayed and evaluated them according to the Chinese Pharmacopoeia, thus laying a foundation for the development of an effective antidote candidate against ricin intoxication.…”

Section: Introductionmentioning

confidence: 99%

Purified Immunoglobulin F(ab′)2 Protects Mice and Rhesus Monkeys against Lethal Ricin Intoxication

et al. 2023

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Objective: Ricin is a highly toxic ribosome-inactivating lectin derived from castor beans. To date, no antidote is available to treat ricin-poisoned patients, and the development of a safe and effective antidote is urgently needed. Methods: First, ricin was prepared and used to construct a mouse model and a rhesus monkey model of ricin intoxication. Second, pepsin-digested F(ab′)2 fragments of serum IgG from horses injected with Freund’s-adjuvanted purified ricin were prepared. Third, the protective efficacy was evaluated in mouse and rhesus monkey models of lethal ricin intoxication. Results: The purity quotient of the prepared ricin and F(ab′)2 fragments exceeded 90% and 85% in the mouse and monkey models, respectively. The LD50 of ricin in mice and rhesus monkeys was 2.7 and 9 μg/kg, respectively. A quantity of 6.25 and 1.85 mg/kg F(ab′)2 was sufficient to treat lethal ricin intoxication in the mice and rhesus monkeys, respectively. Finally, the effect of this therapeutic antibody on peripheral blood immune cells was examined by analysis of peripheral blood immune cells through single cell sequencing. The underlying mechanism was found to involve restraining neutrophil activation, proliferation, and differentiation. Conclusion: Purified F(ab′)2 fragments administered with needle-free devices fully protect mice and rhesus monkeys against lethal doses of ricin intoxication.

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Mosla scabra flavonoids ameliorate the influenza A virus-induced lung injury and water transport abnormality via the inhibition of PRR and AQP signaling pathways in mice

Fang

et al. 2016

Journal of Ethnopharmacology

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Protection from infection with influenza A H7N9 virus in a mouse model by equine neutralizing F(ab′)2

Cited by 3 publications

References 20 publications

Prophylactic Efficacy of Equine Immunoglobulin F(ab′)2 Fragments Against Feline Parvovirus

Prophylactic Efficacy of Equine Immunoglobulin F(ab′)2 Fragments Against Feline Parvovirus

Purified Immunoglobulin F(ab′)2 Protects Mice and Rhesus Monkeys against Lethal Ricin Intoxication

Mosla scabra flavonoids ameliorate the influenza A virus-induced lung injury and water transport abnormality via the inhibition of PRR and AQP signaling pathways in mice

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