Protection of apigenin against acrylonitrile-induced sperm and testis injury in rats: involvement of activation of ASK1-JNK/p38 signaling pathway

Shi, Ying; Bai, Jingwei; Dang, Yuhui; Bai, Qingli; Zheng, Rong; Chen, Jia; Li, Zhilan

doi:10.1093/toxres/tfab017

Cited by 10 publications

(4 citation statements)

References 61 publications

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“…Previous studies have shown that ROS are agonists of MAPK family members [ 12 , 13 ]. Upstreams of JNK are at least two classes of kinases, MAPKKKs (ASK1) and MAPKKs (MKK4, MKK7), which sequentially phosphorylate and activate JNK through a cascade of reactions [ 14 , 15 ]. Zhu et al have demonstrated that tanshinone exerts its cerebral protective effects by scavenging reactive oxygen species (ROS) and inhibiting inflammatory responses during cerebral ischemia-reperfusion injury [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Tanshinone Protects against Spinal Cord Ischemia-Reperfusion Injury by Inhibiting JNK Activity

Zhang

Liu

et al. 2022

Computational Intelligence and Neuroscience

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Spinal cord reperfusion injury as a secondary damage after primary spinal cord injury is an important factor causing nerve cell damage. In this study, we aim to investigate the effects and mechanisms of tanshinone (TAE) in the rabbit spinal cord during ischemia-reperfusion. New Zealand white rabbits were randomly divided into 3 groups: sham-operated group (5 rabbits), ischemia-reperfusion group (0.9% TAE was administered intraperitoneally 30 min before ischemia, and 4 groups of 5 rabbits each according to different time periods of reperfusion: group A reperfused for 0.5 h, group B reperfused for 2 h, group C reperfused for 8 h, and group D reperfused for 24 h), and TAE group (an ischemia-reperfused for 24 h). Group A was reperfused for 0.5 h, group B for 2 h, group C for 8 h, group D for 24 h, and group TAE (TAE was applied 30 min before ischemia reperfusion, grouped as ischemia-reperfusion group). The expression of JNK (c-Jun NH2-terminal Kinase) and phosphorylation-JNK (p-JNK) in spinal cord tissues of each group were detected by Western blot. Light and electron microscopy showed that early apoptosis started in group B in the ischemia-reperfusion group, while early apoptosis appeared only in group D in the tanshinone intervention group. Western blot showed that p-JNK expression started in group B in the ischemia-reperfusion group and gradually increased with the prolongation of ischemia time, while p-JNK expression only increased in group D in the tanshinone intervention group. In the tanshinone intervention group, p-JNK was activated only in group D and its activity was less than that in the ischemia-reperfusion group; the protein expression of JNK did not change significantly in both groups. Spinal cord ischemia-reperfusion can cause spinal cord injury by activating the signaling molecule JNK (MRPKs family), and early tanshinone intervention can partially inhibit this injury. Our finding provides a new idea and theoretical basis for clinical treatment of spinal cord ischemia-reperfusion injury.

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Section: Introductionmentioning

confidence: 99%

Tanshinone Protects against Spinal Cord Ischemia-Reperfusion Injury by Inhibiting JNK Activity

Zhang

Liu

et al. 2022

Computational Intelligence and Neuroscience

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“…The 100 µmol/L dose provided the most protection against apoptosis-induced DNA damage and loss of motility/function. Apigenin may inhibit sperm apoptosis and necrosis, by regulating the balance between pro-apoptotic and anti-apoptotic factors, such as caspases, Bcl-2, and Bax 31 , 34 , 35 . Shi, et al 35 , investigated the protective effects of apigenin against acrylonitrile-induced sperm and testis injury in rats.…”

Section: Discussionmentioning

confidence: 99%

“…Apigenin may inhibit sperm apoptosis and necrosis, by regulating the balance between pro-apoptotic and anti-apoptotic factors, such as caspases, Bcl-2, and Bax 31 , 34 , 35 . Shi, et al 35 , investigated the protective effects of apigenin against acrylonitrile-induced sperm and testis injury in rats. The results suggested that apigenin treatment significantly improved sperm quality and reduced testicular injury in rats exposed to acrylonitrile.…”

Section: Discussionmentioning

confidence: 99%

Apigenin supplementation substantially improves rooster sperm freezability and post-thaw function

Najafi,

Mohammadi,

Sharifi

et al. 2024

Sci Rep

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This pioneering research investigated apigenin potential to augment rooster sperm cryosurvival in an extender model. Apigenin is a natural antioxidant flavonoid showing promise for improved post-thaw sperm function. However, its effects on avian semen cryopreservation remain unexplored. This first study supplemented rooster sperm Lake extender with 0, 50, 100, 200, 400 μmol/L apigenin to determine the optimal concentrations for post-thaw quality. Supplementation with 100 μmol/L apigenin resulted in significant enhancements in total motility (from 41.5% up to 71.5%), progressive motility (18.1% to 29.1%) (p < 0.05), membrane integrity (40% to 68%), mitochondrial function (p < 0.001), viability (37% to 62%) and total antioxidant capacity (p < 0.001) compared to the control. It also substantially reduced percentages of abnormal morphology, reactive oxygen species and apoptosis (p < 0.001). Although 200 μmol/L apigenin significantly enhanced some attributes, effects were markedly lower than 100 μmol/L. Higher doses did not improve cryoprotective parameters. This indicates 100 μmol/L as the optimal apigenin concentration. This represents the first report of apigenin protecting rooster sperm from cryodamage. The natural antioxidant improved post-thaw sperm quality, likely by suppressing oxidative stress and apoptosis. Apigenin shows promise for enhancing rooster sperm cryosurvival.

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“…1,3-Butadiene may be linked to ovarian atrophy, testicular atrophy, and male infertility [ 39 ]. Acrylonitrile, cyanide, vinyl chloride, and ethylene oxide were also recognized as being potentially toxic for reproduction [ 40 , 41 , 42 , 43 , 44 , 45 ]. These studies provided part of the support for our findings.…”

Section: Discussionmentioning

confidence: 99%

Association between Volatile Organic Compound Exposure and Sex Hormones in Adolescents: The Mediating Role of Serum Albumin

Lian,

Guo,

Wang

et al. 2024

Toxics

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The associations between VOCs and sex hormones in adolescents remain unclear, and the role of serum albumin in these associations deserves to be explored. We conducted cross-sectional analyses using generalized linear models (GLMs), weighted quantile sum (WQS) regression, and mediation analysis, based on data from 584 adolescents from the National Health and Nutrition Examination Survey (NHANES). The GLM analyses revealed that seven kinds of mVOCs potentially affected sex hormone levels. According to the WQS regression results, 2-aminothiazoline-4-carboxylic acid (ATCA) was the major contributor to the significant associations of mixed mVOC exposure with testosterone, estradiol, and free androgen index in males; N-acetyl-S-(N-methylcarbamoyl)-L-cysteine (AMCC) was the major contributor to the significant associations of mixed mVOC exposure with sex hormone-binding globulin in males; and N-acetyl-S-(benzyl)-L-cysteine (BMA) was the major contributor to the significant associations of mixed mVOC exposure with the ratio of testosterone to estradiol in females. Moreover, serum albumin could mediate up to 9.2% of the associations between mixed exposure to mVOCs and sex hormones. Our findings could provide a reference for studies on the mechanisms underlying the effects of VOCs on sex hormones in adolescents and emphasize the necessity of reducing exposure to ATCA, AMCC, BMA, and their parent compounds.

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Protection of apigenin against acrylonitrile-induced sperm and testis injury in rats: involvement of activation of ASK1-JNK/p38 signaling pathway

Cited by 10 publications

References 61 publications

Tanshinone Protects against Spinal Cord Ischemia-Reperfusion Injury by Inhibiting JNK Activity

Tanshinone Protects against Spinal Cord Ischemia-Reperfusion Injury by Inhibiting JNK Activity

Apigenin supplementation substantially improves rooster sperm freezability and post-thaw function

Association between Volatile Organic Compound Exposure and Sex Hormones in Adolescents: The Mediating Role of Serum Albumin

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