Protection of cellular DNA from γ-radiation-induced damages and enhancement in DNA repair by troxerutin

Abstract

The effect of troxerutin on gamma-radiation-induced DNA strand breaks in different tissues of mice in vivo and formations of the micronuclei were studied in human peripheral blood lymphocytes ex vivo and mice blood reticulocytes in vivo. Treatments with 1 mM troxerutin significantly inhibited the micronuclei induction in the human lymphocytes. Troxerutin protected the human peripheral blood leucocytes from radiation-induced DNA strand breaks in a concentration dependent manner under ex vivo condition of irradiation (2 Gy). Intraperitoneal administration of troxerutin (175 mg/kg body weight) to mice before and after whole body radiation exposure inhibited micronuclei formation in blood reticulocytes significantly. The administration of different doses (75, 125 and 175 mg/kg body weight) of troxerutin 1 h prior to 4 Gy gamma-radiation exposure showed dose-dependent decrease in the yield of DNA strand breaks in murine blood leucocytes and bone marrow cells. The dose-dependent protection was more pronounced in bone marrow cells than in blood leucocytes. Administration of 175 mg/kg body weight of the drug (i.p.) 1 h prior or immediately after whole body irradiation of mice showed that the decrease in strand breaks depended on the post-irradiation interval at which the analysis was done. The observed time-dependent decrease in the DNA strand breaks could be attributed to enhanced DNA repair in troxerutin administered animals. Thus in addition to anti-erythrocytic, anti-thrombic, fibrinolytic and oedema-protective rheological activity, troxerutin offers protection against gamma-radiation-induced micronuclei formation and DNA strand breaks and enhances repair of radiation-induced DNA strand breaks.

Troxerutin Protects Against Myocardial Ischemia/Reperfusion Injury Via Pi3k/Akt Pathway in Rats

“…It has various biological functions, including improving capillary function, reducing capillary fragility and abnormal leakage, and anti-erythrocytic and anti-thrombotic effects [15]. Troxerutin has protective effects in several tissues, including kidney [16], liver [17] and brain [18].…”

Substance P stimulates the recovery of bone marrow after the irradiation

“…Thus, recent research in the field of radiation biology has focused on avoiding the side effects of radiation and developing more effective therapeutic strategies to permit bone marrow recovery. Several natural and synthetic compounds, including troxerutin (Maurya et al, 2005), flavonoids (Uma Devi et al, 1999), and melatonin (Assayed and Abd El-Aty, 2009) have been examined for their ability to protect against the radiation damage. Among these materials, amifostine is the most powerful radio-protector.…”

Role of Radioprotectors in the Inhibition of DNA Damage and Modulation of DNA Repair After Exposure to Gamma-Radiation

“…1 h prior or immediately after whole body irradiation of mice showed that the decrease in strand breaks depended on the post-irradiation interval at which the analysis was done. Measurement of DNA repair potential of the troxerutin shows that it enhances the DNA repair (Maurya et al 2005a). So the observed time-dependent decrease in the DNA strand breaks could be attributed to enhanced DNA repair in troxerutin administered animals.…”