Protection of Differentiating Neuronal Cells from Amyloid β Peptide-induced Injury by Alkaline Extract of Leaves of Sasa senanensis Rehder

Sakagami, Hiroshi; Tsuji, Mayumi; Tomomura, Mineko; Masuda, Yoshiko; Iwama, Soichi; Nakagawa, Masamichi; Suzuki, Hayato; Tanaka, K.; Abe, Tokiya; Tamura, Nobuaki; Tomomura, Akito; Yokose, Satoshi; Takeshima, Hiroshi; Natori, Takenori; Horiuchi, Misaki; Fujisawa, Tomohiro; Kiuchi, Yuji; Oguchi, Katsuji; Yasui, Toshikazu; Oizumi, Hiroshi; Oizumi, Takaaki

doi:10.21873/invivo.11229

Cited by 13 publications

(11 citation statements)

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“…Table 2 shows the SI (=CC 50 /EC 50 ) value of polyphenols, plant extracts and antioxidants, calculated using the data from our original papers [ 33 , 35 , 38 , 39 ] and the present study ( Table 2 ). The higher the SI value, the stronger the neuroprotective activity would be expected.…”

Section: Resultsmentioning

confidence: 99%

“…The SE showed the highest neuroprotective activity (SI = 37.2, 141.4, >108, <1, <1, 7.1, 5.9, 5.4), followed by EGCG (SI = 6.1, <1, <1, <1). However, resveratrol (SI = <1, ><1, <1, <1), curcumin (SI = <1, <1, <1, <1) and p -coumaric acid (SI = ><1, ><1, ><1, ><1) showed no apparent neuroprotective activity [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

“…SE, a group of three over-the-counter drugs, showed the highest neuroprotective activity against both undifferentiated and differentiated PC12 cells, as well as undifferentiated SH-SY5Y cells. This seems to be linked to growth stimulation at lower doses, known as hormesis [ 40 ], since we have experienced similar growth stimulation of SE towards human gingival epithelial fibroblast (HGEP) [ 41 ] and PC12 cells [ 33 ]. SE showed prominent anti-HIV [ 42 ] and anti-UV activity [ 43 ], like the lignin-carbohydrate complexes extracted with alkaline solution.…”

Section: Discussionmentioning

confidence: 99%

“…If a fresh NGF-containing medium was supplemented by an overlay at the middle stage (without a medium change), comparable numbers of differentiated cells could be harvested after six or seven days, regardless of the types of plates, either non-coated or coated with collagen I or IV. To our surprise, due to its toxicity, the use of poly-lysine coated plates significantly reduced the yield of differentiated cells [ 33 ]. Based on these results, DMEM, supplemented with NGF, was adopted as a regular differentiation induction medium, which was added to the culture plates that were not coated with collagen I, IV, or poly-lysine.…”

Section: Introductionmentioning

confidence: 99%

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Search of Neuroprotective Polyphenols Using the “Overlay” Isolation Method

et al. 2018

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Previous studies of the neuroprotective activity of polyphenols have used ununiform culture systems, making it difficult to compare their neuroprotective potency. We have established a new and simple method for preparing differentiated PC12 cells by removing the toxic coating step. Cells were induced to differentiate with the nerve growth factor (NGF) in a serum-free medium, without a medium change, but with a one-time overlay supplementation of NGF. The optimal inoculation density of the cells was 6–12 × 103 cells/cm2, and the presence of serum inhibited the differentiation. Neuroprotective activity could be quantified by the specific index (SI) value, that is, the ratio of the 50% cytotoxic concentration to the 50% effective concentration. Alkaline extract from the leaves of Sasa senanensis Rehder (SE), having had hormetic growth stimulation, showed the highest SI value, followed by epigallocatechin gallate. The SI value of curcumin and resveratrol was much lower. This simple overly method, that can prepare massive differentiated neuronal cells, may be applicable for the study of the differentiation-associated changes in intracellular metabolites, and the interaction between neuronal cells and physiological factors.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Search of Neuroprotective Polyphenols Using the “Overlay” Isolation Method

et al. 2018

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“…Next, the ability of these inhibitors to exert neuroprotective effect on cells in a controlled-setting was investigated. The pheochromocytoma PC12 cells obtained from rat adrenal medulla were chosen for their extremely high sensitivity to Aβ-associated neurodegeneration, a response higher than the human cell line, SH-SY5Y (Sakagami et al, 2017(Sakagami et al, , 2018. When tested on the PC12 cells, SalB was most efficient in protecting PC12 cells against Aβ42's neurotoxicity, followed by SalA and DWE.…”

Section: Discussionmentioning

confidence: 99%

Alleviatory effects of Danshen, Salvianolic acid A and Salvianolic acid B on PC12 neuronal cells andDrosophila melanogastermodel of Alzheimer’s disease

Tan

Ting

Najimudin

et al. 2020

Preprint

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Alzheimer's disease (AD) is the most common form of neurodegenerative disorder worldwide. Its pathogenesis involves the hallmark aggregation of amyloid-beta (Aβ). Of all the Aβ oligomers formed in the brain, Aβ42 has been found to be the most toxic and aggressive. Despite this, the mechanism behind this disease remains elusive. With the ability to utilize various genetic manipulations, Drosophila melanogaster is ideal in analysing not only cellular characteristics, but also physiological and behavioural traits of human neurodegenerative diseases. Danshen water extract (DWE), obtained from the root of Salvia miltiorrhiza Bunge, was found to have a vast array of beneficial properties. In this study, DWE, and its major components, Salvianolic acid A (SalA) and Salvianolic acid B (SalB) were tested for their abilities to ameliorate Aβ42's effects. DWE, SalA and SalB were confirmed to be able to reduce fibrillation of Aβ42. As Aβ42 causes neurodegeneration on neurons, DWE, SalA and SalB were tested on Aβ42-treated PC12 neuronal cells and were shown to increase cell viability. DWE and its components were then tested on the Drosophila melanogaster AD model and their rescue effects were further characterized. When human Aβ42 was expressed, the Drosophila exhibited degenerated eye structures known as the rough eye phenotype (REP), reduced lifespan and deteriorated locomotor ability. Administration of DWE, SalA and SalB partially reverted the REP, increased the age of AD Drosophila and improved most of the mobility of AD Drosophila. In conclusion, DWE and its components may have therapeutic potential for AD patients and possibly other forms of brain diseases.

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Alleviatory effects of Danshen, Salvianolic acid A and Salvianolic acid B on PC12 neuronal cells and Drosophila melanogaster model of Alzheimer's disease

Tan

Ting

Leow

et al. 2021

Journal of Ethnopharmacology

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Background: Alzheimer's disease (AD) is the most common form of neurodegenerative disorder worldwide. Its pathogenesis involves the hallmark aggregation of amyloid-beta (Aβ). Of all the Aβ oligomers formed in the brain, Aβ42 has been found to be the most toxic and aggressive. Despite this, the mechanism behind this disease remains elusive. Danshen water extract (DWE), obtained from the root of Salvia miltiorrhiza Bunge, was found to have a vast array of bene cial properties. In this study, DWE, and its major components, Salvianolic acid A (SalA) and Salvianolic acid B (SalB) were tested for their abilities to attenuate Aβ42's toxic effects.Methods: The compounds were rst veri ed for their ability to diminish Aβ42 brillation using an in vitro activity assay. Since Aβ42 aggregation results in neuronal degeneration, the potential Aβ42 inhibitors were next evaluated on Aβ42-exposed PC12 neuronal cells as well as the Drosophila melanogaster model organism.Results: DWE, SalA and SalB were shown to be able to reduce brillation of Aβ42. When tested on PC12 neuronal cells, DWE, SalA and SalB ameliorated cells from cell death associated with Aβ42 exposure. Next, DWE and its components were tested on the Drosophila melanogaster AD model and their rescue effects were further characterized. When human Aβ42 was expressed, the Drosophila exhibited degenerated eye structures known as the rough eye phenotype (REP), reduced lifespan and deteriorated locomotor ability. Administration of DWE, SalA and SalB partially reverted the REP, increased the age of AD Drosophila and improved most of the mobility of AD Drosophila. Conclusion:Collectively, DWE and its components may have therapeutic potential for AD patients and possibly other forms of brain diseases.

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Protection of Differentiating Neuronal Cells from Amyloid β Peptide-induced Injury by Alkaline Extract of Leaves of Sasa senanensis Rehder

Cited by 13 publications

References 18 publications

Search of Neuroprotective Polyphenols Using the “Overlay” Isolation Method

Search of Neuroprotective Polyphenols Using the “Overlay” Isolation Method

Alleviatory effects of Danshen, Salvianolic acid A and Salvianolic acid B on PC12 neuronal cells andDrosophila melanogastermodel of Alzheimer’s disease

Alleviatory effects of Danshen, Salvianolic acid A and Salvianolic acid B on PC12 neuronal cells and Drosophila melanogaster model of Alzheimer's disease

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