Protection of human erythrocyte using Crinum asiaticum extract and lycorine from oxidative damage induced by 2-amidinopropane

Abstract

The intention of this investigation was to evaluate the free radical scavenging activity and erythrocyte protective activity of ethanolic extract of Crinum asiaticum (L) and lycorine. The ethanolic extract of C. asiaticum (L) and lycorine were found to have different levels of antioxidant properties in the test models. Both ethanolic extract of C. asiaticum (L) (0.5-2.5 mg/ml) and lycorine (0.010 mg-0.050 mg/ml) increases the percentage of lipid peroxidation inhibition (26.25 ± 0.23% and 19.25 ± 0.23%) and enhances the free radical scavenging activity (20.92 ± 0.22% and 20.52 ± 0.22%), scavenging of hydrogen peroxide (25.67 ± 0.17% and 23.07 ± 0.3%) superoxide anion scavenging activity (27.69 ± 0.16% and 16.09 ± 0.7%) at concentration of 2.5 and 0.050 mg of C. asiaticum (L) and lycorine, respectively. But compared with tocopherol (P < 0.05) less activity was observed by C. asiaticum (L) and lycorine. The ethanolic extract of C. asiaticum (L) and lycorine were normalized to reduce the level of glutathione and also to sustain the status of protein in erythrocytes during the peroxyl radical [2,2-azobis (2-amidinopropane) dihydrochloride (AAPH)] induced oxidative damage in ex vivo model. The present results of the investigations demonstrated that protective nature of the C. asiaticum (L) and lycorine will be considered as a significant natural antioxidant source.

Guazuma ulmifolia Lam. Decreases Oxidative Stress in Blood Cells and Prevents Doxorubicin‐Induced Cardiotoxicity

“…To best understand the biological potential of the G. ulmifolia extracts, we used human blood cells subjected to oxidative stress induced by different oxidant agents. Initially, we used AAPH, a water-soluble azo compound that decomposes at 37°C generating peroxyl radicals (ROO) [ 49 ] responsible for oxidizing erythrocyte membrane lipids and proteins [ 50 ]. Azo compound-derived ROO and those formed physiologically and pathologically in vivo react with biomolecules similarly, facilitating the study of the oxidation kinetics of biological molecules and their possible protection [ 51 ].…”

An overview on the chemical and biological aspects of lycorine alkaloid

“…Lycorine showed significant DPPH scavenging effects [64]. It also exerted protective effects on human erythrocytes against oxidative damage induced by 2-amidinopropane due to its antioxidant nature [65]. Lately, lycorine has been shown to exhibit significant hepatoprotective effects against CCl 4 -induced oxidative stress in Swiss albino mice at 5 mg/kg that were also comparable to Silymarin.…”

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“…Similarly, 92 , Ilavenil et al showed that C. asiaticum L. ethanolic extract and lycorine possess human erythrocyte protective effects against oxidative damage induced by 2-amidinopropane. The effects were attributed to their antioxidant nature.…”