Protection of immunized and previously infected chimpanzees challenged with Mycoplasma pneumoniae

Barile, Michael F.; Grabowski, Marion W.; Kapatais-Zoumbois, Kaity; Brown, Bobby G.; Hu, Ping-Chuan; Chandler, D. K. F.

doi:10.1016/0264-410x(94)90220-8

Cited by 14 publications

(13 citation statements)

References 26 publications

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“…Perhaps the specific antibodies produced by the host give protection against a reinfection with M. genitalium or M. pneumoniae as suggested in many studies with animal models [42][43][44][45]. This could explain the greater prevalence of M. pneumoniae pneumonia in children and young adults rather than adults and elderly people [46,47] and is in agreement with the proportional correlation between increasing age and the presence of antibodies to M. pneumoniae [48].…”

Section: Discussionsupporting

confidence: 72%

Adhesion and inhibition assay of Mycoplasma genitalium and M. pneumoniae by immunofluorescence microscopy

Svenstrup¹,

Nielsen²,

Drasbek

et al. 2002

Journal of Medical Microbiology

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Adhesion of Mycoplasma pneumoniae and the closely related M. genitalium to HEp-2 cells was investigated. The main surface proteins known to be involved in adhesion are P1 of M. pneumoniae and its homologue, MgPa, of M. genitalium. Both proteins are also immunodominant proteins. Protein P116 is another immunodominant protein of M. pneumoniae. These immunogenic proteins were investigated for their surface exposure and involvement in adhesion to host epithelial cells. Immunofluorescence microscopy (IFM) was used to detect M. pneumoniae and M. genitalium adhering to HEp-2 cells. Monospecific antibodies were produced against fragments of the surface proteins lacking tryptophan stop codons and were used for adhesion detection, surface exposure and adhesion inhibition IFM assays. Three monospecific antibodies were made against MgPa covering regions in the N-terminal, the middle and the C-terminal part; two monospecific antibodies were produced against P1 covering regions of the N-and the C-terminal part and one monospecific antibody was made against most of P116. Only the C-terminal parts of P1 and MgPa were surface exposed and blocking of these regions with the monospecific antibody resulted in inhibition of cytadsorption. Protein P116 was shown to be surface exposed and an essential protein involved in adhesion because the anti-P116 antibody prevented attachment of M. pneumoniae to the HEp-2 cells independently of P1. This study adds to the understanding of the molecular biology of M. genitalium and M. pneumoniae and presents a method to study the proteins involved in adhesion of these mycoplasmas.

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Section: Discussionsupporting

confidence: 72%

Adhesion and inhibition assay of Mycoplasma genitalium and M. pneumoniae by immunofluorescence microscopy

Svenstrup¹,

Nielsen²,

Drasbek

et al. 2002

Journal of Medical Microbiology

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“…The sera examined in this study were collected in two previous studies (4,5,7). In the first study, six chimpanzees were experimentally infected with Mycoplasma pneumoniae and showed overt signs of disease that corresponded with peak lung colonization, the development of cold agglutinins, and positive X-ray findings (4).…”

mentioning

confidence: 99%

Immunoblot analyses of chimpanzee sera after infection and after immunization and challenge with Mycoplasma pneumoniae

Franzoso

Meloni

et al. 1994

Infect Immun

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Consecutive weekly or biweekly serum specimens obtained during a 3or 4-month study from 16 chimpanzees were examined by immunoblot analyses to identify the immunogenic components of Mycoplasma pneumoniae. Six experimentally infected chimpanzees showed significant signs of overt disease, including cough, pharyngitis, rhinitis, fever, and loss of appetite. The sera of these infected chimpanzees recognized from 17 to 20 protein bands. Two control chimpanzees that were not inoculated were included in the study. Three chimpanzees immunized with a formalin-inactivated OSU-1A vaccine and three chimpanzees immunized with an experimental acellular vaccine showed minimal signs of disease on challenge. After challenge, the serum immunoblot responses of the immunized chimpanzees were similar to those of the infected chimpanzees. Before challenge, the sera of two previously infected chimpanzees recognized protein bands of 169 (which comigrated with the P1 adhesin), 148, 130, 117, 86, 61, 44, 35, 30, and 29 kDa. After challenge, the previously infected chimpanzees showed the most intense serum immunoblot responses and were most protected against colonization and disease. The sera from each of the 16 chimpanzees examined recognized a large number of immunogenic components, and the serum immunoblot responses were virtually identical to those of patients. Sera from each chimpanzee and patient recognized 169-, 148-, 130-, 117-, 86-, 44-, and 35-kDa bands and many of them recognized 67-, 63-, 61-, 56-, 32-, 30-, and 29-kDa protein bands.

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“…As expected, immunity does play a role in protection from mycoplasma diseases. Vaccination against mycoplasma disease is possible (33)(34)(35)(36)(37)(38)(39)(40)(41). Mucosal IgA responses are likely important in resistance to disease associated with mycoplasma infection of mucosal surfaces (42,43).…”

Section: The Complexity Of Immune Interactions With Mycoplasmamentioning

confidence: 99%

T lymphocyte responses are critical determinants in the pathogenesis and resistance to mycoplasma respiratory disease

Jones

Simecka

2003

Front Biosci

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Mycoplasmas are responsible for many human and animal respiratory diseases and have a tremendous economic and health impact worldwide. Currently, there is no vaccine against any animal or human mycoplasma species. Despite the vast amount of research, the mechanisms that control hosts' resistance and susceptibility to mycoplasma infection remains unclear. Immune responses, particularly T lymphocyte responses, are believed to be critical players in the pathogenesis of mycoplasma disease. In this review, we will highlight the potential roles of T lymphocytes as influential mediators in animal and human mycoplasma pathogenesis and resistance infection.

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Protection of immunized and previously infected chimpanzees challenged with Mycoplasma pneumoniae

Cited by 14 publications

References 26 publications

Adhesion and inhibition assay of Mycoplasma genitalium and M. pneumoniae by immunofluorescence microscopy

Adhesion and inhibition assay of Mycoplasma genitalium and M. pneumoniae by immunofluorescence microscopy

Immunoblot analyses of chimpanzee sera after infection and after immunization and challenge with Mycoplasma pneumoniae

T lymphocyte responses are critical determinants in the pathogenesis and resistance to mycoplasma respiratory disease

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