Protection of lung fibroblast MRC-5 cells against hydrogen peroxide-induced oxidative damage by 0.1–2.8kDa antioxidative peptides isolated from whey protein hydrolysate

Protective effects against H2O2-induced oxidative damage in lung fibroblast cell by peptide isolated from plasma albumin hydrolysate

“…H 2 O 2 is generated from a variety of sources under oxidative stress, which has the ability to diffuse freely in and out of cells and tissues [37]. Similar to our findings, Kong [32] found that the survival rate of MRC-5 cell exposed to H 2 O 2 can be improved to 66.1 % with 100 mg/ ml of peptides from whey protein hydrolysate. It has been suggested that the antioxidant ability of biopeptides in vitro depends on peptide size, the amino acid composition of peptide and the presence of free amino acids within the hydrolysate [38].…”

“…When it comes to the activities of SOD, CAT and GPx, the result is similar to that of whey protein reported by Kong [32], suggesting that those peptides can promote the activity of intracellular antioxidative enzymes. Interestingly, the activities of SOD, CAT and GPx treated with high concentration of P4 are significantly higher than that of further purified fraction P4b and unpurified fraction AHA (P < 0.05).…”

“…For instance, Guo [31] isolated 29 antioxidative peptides from royal jelly protein hydrolysate and found that small peptides with 2-4 amino acid residues had strong antioxidant activity. Furthermore, the presence of appropriate hydrophobic amino acid residues is essential for the absorption and distribution of peptides in the cell, thereby promoting their efficacy as antioxidant agents [32]. Our results demonstrated that the seven peptides purified from AHA are composed of 4-8 amino acid residues and all of them are hydrophobic.…”

“…These results agree with the protective effect of peptide on cells viability damaged by H 2 O 2 and RP, where the highest concentration of P4 also demonstrated better activities than P4b and AHA. Similarly, casein phosphopeptides, peptide lunasin and hydrolysate of eggshell membrane proteins have been demonstrated to exert this effect in H 2 O 2 -induced Caco-2 cells [20,41], while casein and whey hydrolysates are confirmed of protective effects on H 2 O 2 -induced hepatic HepG2 and MRC-5 cell line, respectively [32,42].…”

Emerging trends in nutraceutical applications of whey protein and its derivatives

“…Alcalasehydrolysed whey protein was screened for anti-oxidative peptides. Two fragments, P4 and P4c (a pentapeptide Val-HisLeu-Lys-Pro) demonstrated significant protection of human lung fibroblast MRC-5 cells from H 2 O 2 abuse (Kong et al 2012). The anti-inflammatory effect of a new enteral diet (tube feeding) MHN-02, rich in antioxidants and whey peptide was evaluated in rats (Takayanagi et al 2011).…”