Protection of Neural Stem Cells From Genotoxic Effects by Factors Secreted by Mesenchymal Stem Cells

Posypanova, G.A.; Ratushnyak, M. G.; Vysotskaya, O. V.; Глухов, А.И.; Semochkina, Yu. P.; Rodina, A. V.; Moskaleva, E. Yu.

doi:10.29296/24999490-2018-06-05

Cited by 3 publications

(1 citation statement)

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“…The relatively short half-life of 99m Tc might play a role in this since the amount of activity is quite quickly reduced to background radiation levels. On the other hand, limited doses of γ radiation are known to stimulate the proliferation of mesenchymal stem cells from mouse brains, up to a dose of 100 mGy [33]. However, at the end of the 3-week differentiation period, the cell count revealed no significant difference between Groups 1 and 7.…”

Section: Experiments 3: Evaluation Of the Repeated 99m Tc-hdp Labelin...mentioning

confidence: 87%

99mTc-HDP Labeling—A Non-Destructive Method for Real-Time Surveillance of the Osteogenic Differentiation Potential of hMSC during Ongoing Cell Cultures

Hofmann

Borcherding

Thiel

et al. 2022

IJMS

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99-Metastabil Technetium (99mTc) is a radiopharmaceutical widely used in skeletal scintigraphy. Recent publications show it can also be used to determine the osteogenic potential of human mesenchymal stem cells (hMSCs) by binding to hydroxyapatite formed during bone tissue engineering. This field lacks non-destructive methods to track live osteogenic differentiation of hMSCs. However, no data about the uptake kinetics of 99mTc and its effect on osteogenesis of hMSCs have been published yet. We therefore evaluated the saturation time of 99mTc by incubating hMSC cultures for different periods, and the saturation concentration by using different amounts of 99mTc activity for incubation. The influence of 99mTc on osteogenic potential of hMSCs was then evaluated by labeling a continuous hMSC culture three times over the course of 3 weeks, and comparing the findings to cultures labeled once. Our findings show that 99mTc saturation time is less than 0.25 h, and saturation concentration is between 750 and 1000 MBq. Repeated exposure to γ-radiation emitted by 99mTc had no negative effects on hMSC cultures. These new insights can be used to make this highly promising method broadly available to support researchers in the field of bone tissue engineering using this method to track and evaluate, in real-time, the osteogenic differentiation of hMSC, without any negative influence on the cell viability, or their osteogenic differentiation potential.

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Section: Experiments 3: Evaluation Of the Repeated 99m Tc-hdp Labelin...mentioning

confidence: 87%

99mTc-HDP Labeling—A Non-Destructive Method for Real-Time Surveillance of the Osteogenic Differentiation Potential of hMSC during Ongoing Cell Cultures

Hofmann

Borcherding

Thiel

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

Improved Survival and Regeneration of Irradiated Mouse Neural Stem Cells after Co-Culturing with Non-Irradiated Mouse Neural Stem Cells or Mesenchymal Stem Cells from the Adipose Tissue

et al. 2021

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The Use of Neural Stem Cells-Derived Exosomes to Prevent Late Radiation-Induced Cognitive Impairments in Mice

et al. 2023

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Protection of Neural Stem Cells From Genotoxic Effects by Factors Secreted by Mesenchymal Stem Cells

Cited by 3 publications

References 0 publications

99mTc-HDP Labeling—A Non-Destructive Method for Real-Time Surveillance of the Osteogenic Differentiation Potential of hMSC during Ongoing Cell Cultures

99mTc-HDP Labeling—A Non-Destructive Method for Real-Time Surveillance of the Osteogenic Differentiation Potential of hMSC during Ongoing Cell Cultures

Improved Survival and Regeneration of Irradiated Mouse Neural Stem Cells after Co-Culturing with Non-Irradiated Mouse Neural Stem Cells or Mesenchymal Stem Cells from the Adipose Tissue

The Use of Neural Stem Cells-Derived Exosomes to Prevent Late Radiation-Induced Cognitive Impairments in Mice

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