Protection of OK‐432, a <i>Streptococcus pyogenes</i> Preparation, against Lethal Infection of Mice with Herpes Simplex Virus

Harada, Yoko; Kurokawa, Eri; Endo, Hisao; Kimura, Takao; Saito, Motoo; Sugawara, Yutaka; Irie, Hiroshi; Ito, Kyogo; Fujii, Masahiko; Shimizu, Yasutomo; Kumagai, Katsuo

doi:10.1111/j.1348-0421.1989.tb01996.x

Cited by 6 publications

(1 citation statement)

References 20 publications

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“…Anti-mouse thymocyte serum and anti-asialo GM1 serum were purchased from Wako Pure Chemicals Co. Ltd. Silica (particle size, 0.01 ,um) was obtained from Sigma Chemical Co. Ltd. The silica powder was sterilized in an autoclave and suspended at a concentration of 40 mg/ml in EMEM (12).…”

Section: Materlils and Methodsmentioning

confidence: 99%

Efficacy of oral administration of live herpes simplex virus type 1 as a vaccine

Irie

Harada

Kataoka

et al. 1992

J Virol

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Mice given herpes simplex virus type 1 (HSV-1) (Miyama +GC strain) intragastrically via a stainless-steel cannula were rendered immune to subsequent lethal intraperitoneal (i.p.) challenge with HSV-1. The orally administered HSV-1 was completely inactivated in the stomach within a few minutes of inoculation. However, systemic immunity was established 14 days after oral inoculation with the virus and retained for up to 6 months. The mechanisms of establishing systemic immunity were investigated by means of adoptive transfer comparisons. When splenic cells from HSV-1-immunized mice were transplanted into nonimmunized mice, all of the recipient mice survived after a lethal i.p. challenge with the virus. Immunity was not established in antithymocyte serum-treated mice or by transfer of serum from immunized to nonimmunized mice. In addition, all HSV-1-immunized mice died after lethal challenge with HSV-2 and influenza virus A. These findings suggest that the immunity was virus specific, with T lymphocytes playing a major role in its establishment. The present study therefore supports the possibility of oral immunization with live HSV-1 as a vaccine. * Corresponding author. dai, Japan. The virulent virus was obtained by passage in green monkey kidney (GMK) cells and was stored at-80°C (37). Before use, the virus was adjusted to an appropriate inoculum dose with phosphate-buffered saline (PBS) solution. HSV-2 (YS-4 strain) (24, 29

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Section: Materlils and Methodsmentioning

confidence: 99%