Protection of prenylated flavonoids from mori cortex radicis (Moraceae) against nitric oxide-induced cell death in neuroblastoma SH-SY5Y cells

Abstract

Seven prenylated flavanoids, licoflavone C (1), cyclomulberrin (2), neocyclomorusin (3), sanggenon I (4), morusin (5), kuwanon U (6) and kuwanon E (7), and three 2-arylbenzofurans, moracin P (8), moracin O (9), and mulberrofuran Q (10) were isolated from the MeOH extract of Mori Cortex Radicis. Among these, compounds 2-7 enhanced cell viability in a dose-dependent manner against sodium nitroprusside-induced cell death in neuroblastoma SH-SY5Y cells, which was measured by MTT reduction assay (EC(50) values of 4.4, 5.6, 8.0, 6.4, 8.7, and 11.9 μg/mL, respectively). Among 10 compounds, C-3 prenylated flavones (2, 3, and 5) and prenylated flavanones (4, 6, and 7) showed cell protection. However, compound 1 which lacks the prenyl group at C-3 and three 2-arylbenzofurans (8-10) did not show protective effect. The order of cell protection was as follow: C-3 prenylated flavones (2, 3, and 5) > prenylated flavanones (4, 6, and 7) > 2-arylbenzofurans (8-10) and flavone (1). From this result, we show that some prenylated flavones and flavanones might protect neuronal cells against nitrosative stress-mediated cell death. Even though further evaluations are necessary in vitro and in vivo study, we carefully suggest that some prenylated flavonoids from Mori Cortex Radicis might protect neuronal cells from neurodegenerative diseases.

Protective Effects of Morus Root Extract (MRE) Against Lipopolysaccharide-Activated RAW264.7 Cells and CCl4-Induced Mouse Hepatic Damage

“…Soonthornsit et al reported that oxyresveratrol at concentrations of 5 and 10 μg/mL inhibited LPS-induced nitrite production and the expression of iNOS and COX-2 [12]. Morusin, a prenylated flavonoid, potentially has neuroprotective, anticancer, antioxidative, and ant-colitis activities [21][22][23][24]. In this study, the anti-inflammatory effect of ethanol extract from the dried root of M. australis (MRE) was evaluated in vitro and in vivo.…”

Prevention of Disuse Muscle Atrophy by Dietary Ingestion of 8-Prenylnaringenin in Denervated Mice

“…Prenylflavanones extracted from the roots of Sophora flavescens were found to possess antibacterial and anti-androgen activities [6]. Prenylflavones from Psoralea corylifolia and Mori Cortex Radics suppressed the production of nitric oxide in nerve cells [7], [8]. Prenylation has been demonstrated to enhance the estrogenic activity of (1) naringenin [9] and the tyrosinase activity of luteolin [10].…”

Two new phenolic constituents from the root bark of Morus alba L. and their cardioprotective activity

“…Prenylated flavonoids from M. alba may protect neuronal cells from neurodegenerative diseases (Lee et al 2012;Jung et al 2015). Previous studies have found Moracin O and moracin P from M. alba possess cytoprotective properties against DOXinduced cell death of H9c2 cells.…”