Protective Effect of (4-Methoxybenzylidene)-(3-methoxynophenyl)amine against Neuronal Cell Death Induced by Oxygen and Glucose Deprivation in Rat Organotypic Hippocampal Slice Culture

Abstract

Resveratrol (trans-3,4',5-trihydroxystilbene) is a natural phytoalexin found in grape skin, and has been suggested to be an antioxidant agent, an anticancer agent and a cardioprotective agent. In particular, recent experimental evidence has demonstrated that resveratrol exhibits neuroprotective effects in various assay systems. During the study on the resveratrol derivatives, we found that (4-methoxybenzylidene)-(3-methoxyphenyl)amine (MBMPA), which has blocked free phenolic groups, strongly protects neuronal cells against ischemic damage on a higher activity than resveratrol. The MBMPA potently reduced the level of neuronal cell death in an oxygen and glucose deprivation-exposed rat organotypic hippocampal slice culture. In addition, ATP depletion following the onset of oxygen and glucose deprivation in an adult hippocampal slice was blocked by the MBMPA treatment. These results suggest that MBMPA has a neuroprotective effect on an in vitro ischemia model, and may be useful for treating stroke.

Antidepressants are neuroprotective against nutrient deprivation stress in rat hippocampal neurons

“…, 2009), choline (McCann et al. , 2006) and glucose (Choi et al. , 2007) deficiencies are detrimental to both brain structural development and function.…”

Resveratrol analog 4-[2-(3,5-dimethoxyphenyl)vinyl]pyridine reduces differentiation of the 3T3-L1 adipocyte

“…Resveratrol has been reported to have various physiological activities including an anti-obesity effect, but has the problem of low bioavailability (Wenzel & Somoza, 2005;Walle, 2011). As such, many studies have been conducted to discover resveratrol derivatives with higher physiological activity and bioavailability; some resveratrol derivatives with considerably higher effects, compared to resveratrol, on cytochrome P450 inhibition, tyrosinase inhibition, and neuro-protective action have been reported, and are being derived as potential materials for the development of new drugs of natural origin (Shin et al, 1998;Kim et al, 2002;Choi et al, 2007). In this context, DPVP, a nonpolar derivative of resveratrol, exerted inhibitory effects on 3T3-L1 adipocyte differentiation and FAS expression at concentrations lower than 25 µM, compared to resveratrol.…”

Positive Roles of Resveratrol in Early Development of Testicular Germ Cells against Maternal Restraint Stress in Mice

“…Furthermore, several studies have shown that oral RES is well absorbed and metabolized rapidly without significant toxicity [18,19]. Found with good concentration in the grape's skin and seed, it occurs in a trans or a cis isoform [20] with biological effects such as an antioxidant via prevention of lipid peroxidation [21], neuronal cell death reduction [22], and reduction in traumatic brain injury [23]. It is still unknown whether the apoptosis of testis germ cells including neuronal damage in offspring is also due to increased MRS.…”