Protective effect of a<i>Phyllanthus orbicularis</i>aqueous extract against UVB light in human cells

Vernhes, Marioly; González-Pumariega, Maribel; Andrade, Luciana Nogueira de Sousa; Schuch, André Passaglia; Lima-Bessa, Keronninn M.; Menck, Carlos Frederico Martins; Sánchez-Lamar, Ángel

doi:10.3109/13880209.2012.695800

Cited by 11 publications

(9 citation statements)

References 33 publications

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“…In this research, the protection of DNA against UVA, UVB, and solar radiation by POE 0 s UV-filter action has been shown. These results, together with previous investigation in different biomodels and experimental designs, (16)(17)(18) patent the multifunction achievement of P. orbicularis as DNA photoprotector. This extract is a wide font of phytocompounds capable of protecting DNA against UV artificial and solar radiation by different modes of action.…”

Section: Discussionsupporting

confidence: 78%

“…In plasmidic DNA, similar physical mechanism and ROS scavenger action are proposed in pBluescript SK II exposed to UVB and UVC light . Against UVB radiation in human cell model, the bioantimutagenic action of POE is through the NER modulation . In this work, we have demonstrated the protective properties of the extract only because of its physical properties.…”

Section: Discussionmentioning

confidence: 66%

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Genoprotective Effect of Phyllanthus orbicularis Extract Against UVA, UVB, and Solar Radiation

Tamayo

Schuch

Yagura

et al. 2018

Photochem & Photobiology

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One approach to protect the human skin against harmful effects of solar ultraviolet (UV) radiation was to use natural products as photoprotectors. In this work, the extract from specie Phyllanthus orbicularis K was evaluated as a protective agent against the photodamage by UVB, UVA artificial lamps, and environmental sunlight exposure. The plasmid DNA solutions were exposed to radiations using the DNA dosimeter system in the presence of plant extract. The DNA repair enzymes, Escherichia coli Formamidopyrimidine-DNA glycosylase (Fpg) and T4 bacteriophage endonuclease V (T4-endo V), were employed to discriminate oxidized DNA damage and cyclobutane pyrimidine dimers (CPD), respectively. The supercoiled and relaxed forms of DNA were separated through electrophoretic migration in agarose gels. These DNA forms were quantified to determine strand break, representing the types of lesion levels. The results showed that, in the presence of P. orbicularis extract, the CPD and oxidative damage were reduced in irradiated DNA samples. The photoprotective effect of extract was more evident for UVB and sunlight radiation than for UVA. This work documented the UV absorbing properties of P. orbicularis aqueous extract and opened up new vistas in its characterization as protective agent against DNA damage induced by environmental sunlight radiation.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 66%

Genoprotective Effect of Phyllanthus orbicularis Extract Against UVA, UVB, and Solar Radiation

Tamayo

Schuch

Yagura

et al. 2018

Photochem & Photobiology

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“…Cell apoptosis is an initiative cell death under gene regulation and can regulate the balance of cell proliferation in life process [33]. In general, apoptotic cells have obvious morphological changes in nucleus and cytoplasm, including bubbling of cell membrane, degradation of cell nuclei with widespread damage of chromatin, and fragments of DNA caused by Ca 2+ dependent endonuclease [34].…”

Section: Discussionmentioning

confidence: 99%

CD59 Underlines the Antiatherosclerotic Effects of C-Phycocyanin on Mice

Chu

et al. 2013

BioMed Research International

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The effects of C-phycocyanin (C-PC) on atherosclerosis and the regulatory effects of CD59 gene on anti-atherosclerotic roles of C-PC were investigated. Apolipoprotein E knockout (ApoE(−/−)) mice were randomly divided into four groups: control group, C-PC treatment group, CD59 transfection group and C-PC+CD59 synergy group. The mice were fed with high-fat-diet and treated with drug intervention at the same time. Results showed the atherosclerotic mouse model was successfully established. CD59 was over-expressed in blood and tissue cells. Single CD59 or C-PC could reduce blood lipid levels and promote the expression of anti-apoptotic Bcl-2 but inhibit pro-apoptotic Fas proteins in endothelial cells. The expression levels of cell cycle protein D1 (Cyclin D1) and mRNA levels of cyclin dependent protein kinase 4 (CDK4) in smooth muscle cells were restrained by CD59 and C-PC. CD59 or C-PC alone could inhibit the formation of atherosclerotic plaque by suppressing MMP-2 protein expression. In addition, C-PC could promote CD59 expression. So both CD59 and C-PC could inhibit the progress of atherosclerosis, and the anti-atherosclerotic effects of C-PC might be fulfilled by promoting CD59 expression, preventing smooth muscle cell proliferation and the apoptosis of endothelial cells, reducing blood fat levels, and at last inhibiting the development of atherosclerosis.

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“…ed to the extract ability to modulate the NER system [7] One important approach to identify compounds responsible for the observed protective effect can be reached through the study of chemical fractions obtained from the plant extract.…”

Section: Introductionmentioning

confidence: 99%

Photoprotection of Fractions Obtained from Phyllanthus Orbicularis Extract against UVB -Induced DNA Damage

2018

DERM

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The use of plants as sources of photochemopreventive agents has been increased in the last years. The assessment of photoprotective properties of plant extracts improves the development of pharmaceutical and cosmetic products. In the present work, we evaluate the effect of Phyllanthus orbicularis extracts against UVB-induced damage to DNA. The total aqueous extract of this plant (POE) and chemical fractions obtained from it: aqueous (AF), chloroformic (CF) and dichloromethane (DF) were assessed. For this, plasmid DNA solutions were exposed to UVB radiation in presence of plant extracts. The DNA repair enzymes Formamidopyrimidine-DNA glycosylase (Fpg) and T4 bacteriophage endonuclease V (T4-endo V) were employed to distinguish oxidized DNA and cyclobutane pyrimidine dimer (CPD) lesions. The supercoiled and relaxed forms of DNA were separated through electrophoretic migration in agarose gels. Additionally, the UVB wavelength transmittances of total extract and chemical fractions of P.orbicularis were measured. The results showed that POE and AF protect DNA against the damage induced by UVB light. This protective effect is related to the antioxidants and absorptive properties of some flavonoids present in the extracts.

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Protective effect of aPhyllanthus orbicularisaqueous extract against UVB light in human cells

Abstract: P. orbicularis aqueous extract protects human cells against UVB damage. This protective effect is through the modulation of DNA repair effectiveness.

Cited by 11 publications

References 33 publications

Genoprotective Effect of Phyllanthus orbicularis Extract Against UVA, UVB, and Solar Radiation

Genoprotective Effect of Phyllanthus orbicularis Extract Against UVA, UVB, and Solar Radiation

CD59 Underlines the Antiatherosclerotic Effects of C-Phycocyanin on Mice

Photoprotection of Fractions Obtained from Phyllanthus Orbicularis Extract against UVB -Induced DNA Damage

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