Protective effect of black raspberry seed containing anthocyanins against oxidative damage to DNA, protein, and lipid

Abstract

This study aimed to determine bioactive components and radical scavenging capacity of black raspberry seed extracts as byproducts obtaining during the juice (FSE) and wine (WSE) making process. Cyanidin-3-O-rutinoside was identified as a major anthocyanin and the total anthocyanin contents of fresh and wine seed were 78.24 and 41.61 mg/100 g of dry weight, respectively. The total phenolic and flavonoid contents of FSE and WSE were 2.31 g gallic acid equivalent (GAE) and 360.95 mg catechin equivalent (CE), and 2.44 g GAE and 379.54 mg CE per 100 g dry weight, respectively. The oxygen radical absorbance capacity (ORAC) values were 1041.9 μM TE/g for FSE and 1060.4 μM TE/g for WSE. Pretreatment of the FSE and WSE inhibited the generation of intracellular reactive oxygen species (ROS), DNA and protein damage induced by hydroxyl radicals, and Fe(3+)/ascorbic acid-induced lipid peroxidation in a dose dependent manner. WSE more effectively protected from oxidative damage than FSE. Results from the current study suggest that black raspberry seeds as byproducts from juice and wine processing could be potential sources for natural antioxidants.

Novel Insights into Corema album Berries: Vibrational Profile and Biological Activity

“…Moreover, only the BSR extract presented the capacity to inhibit lipid peroxidation (Table 2), though to a much lower extent than the standard antioxidant BHT, which could be explained by the higher content in polyphenolic compounds in this extract. These results are comparable to those previously reported for black raspberry seed residues extracts (after oil extraction) with EC 50 values ranging from 1.132 to 1.255 mg/mL [39].…”

Metabolic profiling reveals reprogramming of lipid metabolic pathways in treatment of polycystic ovary syndrome with 3-iodothyronamine

“…In addition, a Ascorbic acid acts like an antioxidant that protects against lipid peroxidation (Choi et al. ), or functions as an indirect antioxidant, providing electrons to generate reactive forms of other antioxidants such as glutathione (Bourges et al. ).…”

“…Depletion of glutathione is shown during excess mitochondrial cholesterol loading in a mouse model (Mari et al 2006), while reduction in ascorbate level is associated with pathological accumulation of cholesterol and TG in liver and other tissues (Nambisan and Kurup 1975;Holloway and Rivers 1981). In addition, a Ascorbic acid acts like an antioxidant that protects against lipid peroxidation (Choi et al 2016), or functions as an indirect antioxidant, providing electrons to generate reactive forms of other antioxidants such as glutathione (Bourges et al 2005). Taken together, increased levels of glutathione in liver and ascorbic acid in muscle indicate that the oxidative environment in the tissues are reduced despite increased lipid oxidation.…”

Determination of anthocyanins and non-anthocyanin polyphenols by ultra performance liquid chromatography/electrospray ionization mass spectrometry (UPLC/ESI–MS) in jussara (Euterpe edulis) extracts

“…1a). All analyzed anthocyanins are found in many other fruits, for example black raspberry, assai, mulberry, strawberry and others (Choi et al 2016, Wu et al 2011, Silva et al 2007). Dias et al (2012) developed an UPLC method for anthocyanins analysis from assai, using a HSS C18 column (from Waters), that provided a separation of seven anthocyanins within 17 min of total run.…”

“…A critical factor for quality evaluation of polyphenolicrich products is the quantification and identification of their compounds. Advanced separation techniques such as high performance liquid chromatography (HPLC), coupled to photodiode array detection (PDA) and or to mass spectrometry (MS) have been used to analyze anthocyanins and non-anthocyanin phenolic compounds that always co-exist in plant extracts, wine, fruit juices, and other products (Pradhan and Saha 2016;Loypimai et al 2016;Choi et al 2016;Borges et al 2013;Huang et al 2009). The ultraviolet/visible (UV/Vis) molecular absorbance measured by PDA is commonly used to distinguish anthocyanins from non-anthocyanin phenolics, since anthocyanins have maxima absorption within 480-540 nm range and the other phenolics only absorb at wavelengths below 400 nm.…”