Protective effect of bovine lactoferrin against Cronobacter sakazakii in human intestinal Caco-2/TC7 cells

Abad, Inés; Sangüesa, Andrea; Ubieto, María; Carramiñana, Juan J.; Pérez, María Dolores; Buey, Berta; Mesonero, José E.; Grasa, Laura; Sánchez, Lourdes

doi:10.1016/j.idairyj.2022.105428

Cited by 14 publications

(5 citation statements)

References 62 publications

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“…In the study by Harouna et al ( 2015 ), it was shown that iron-saturated LF did not have antibacterial activity against this pathogen; therefore, the antibacterial effect of native LF was probably due to its ability to sequester iron from the medium. Furthermore, in the study by Abad et al ( 2022 ) we showed that native LF was effective against C. sakazakii both in the exponential and in the stationary phase of growth.…”

Section: Resultsmentioning

confidence: 74%

“…In our previous studies, we have demonstrated that native LF had an antibacterial effect against C. sakazakii (Harouna et al 2015 ; Abad et al 2022 ). In the study by Harouna et al ( 2015 ), it was shown that iron-saturated LF did not have antibacterial activity against this pathogen; therefore, the antibacterial effect of native LF was probably due to its ability to sequester iron from the medium.…”

Section: Resultsmentioning

confidence: 94%

“…For the reference stock, the bacteria were fixed to porous rings and stored in cryovials at − 80 °C. To cultivate C. sakazakii , we followed the procedure described in our previous study (Abad et al 2022 ).…”

Section: Methodsmentioning

confidence: 99%

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Effect of in vitro gastrointestinal digestion on the antibacterial activity of bioactive dairy formulas supplemented with lactoferrin against Cronobacter sakazakii

et al. 2022

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Milk is a source of proteins with high nutritional value and relevant biological activities. Bioactive milk proteins, like lactoferrin, are important for newborn development and can also be used as ingredients in functional products to improve health. Lactoferrin is essential in infant’s diet, since protects against infections and promotes immune system maturation. Bovine lactoferrin is used to supplement formula milk in order to strengthen baby’s defences against some pathogenic bacteria. Thus, lactoferrin supplemented formula can be a barrier against emergent pathogens, such as Cronobacter sakazakii, which has caused great concern in the last few years. Milk proteins generate bioactive peptides in the digestion process, and it is known that industrial processing can modify their susceptibility to digestion. Treatments such as heating have been shown to denature whey proteins and make them more easily digestible. Therefore, the aim of this study was to analyze the effect of technological treatments and gastrointestinal digestion on the antibacterial activity against C. sakazakii of proteins present in dairy formulas supplemented with lactoferrin. Commercial bovine lactoferrin has been shown to have antibacterial activity against C. sakazakii, both in the native state and after static in vitro gastrointestinal digestion. In addition, the digests obtained from dairy formulas subjected to technological treatments, either homogenization or pasteurization, have higher antibacterial activity than non-treated formulas. The release of low molecular weight peptides during the in vitro gastric digestion is probably the cause that would explain the enhanced antibacterial activity of the digested dairy formulas.

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Section: Resultsmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 94%

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Effect of in vitro gastrointestinal digestion on the antibacterial activity of bioactive dairy formulas supplemented with lactoferrin against Cronobacter sakazakii

et al. 2022

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“…Bovine LF was kindly donated by Tatua Nutritionals (Morrinsville, New Zealand). This LF was used in our previous assays, in which iron saturation, purity, and content of LPS were determined [30].…”

Section: Chemicals and Preparation Of Milk Samplesmentioning

confidence: 99%

Dairy By-Products and Lactoferrin Exert Antioxidant and Antigenotoxic Activity on Intestinal and Hepatic Cells

Abad

Vignard

Bouchenot

et al. 2023

Foods

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The dairy industry generates a large volume of by-products containing bioactive compounds that may have added value. The aim of this study was to evaluate the antioxidant and antigenotoxic effects of milk-derived products, such as whey, buttermilk, and lactoferrin, in two human cell lines: Caco-2 as an intestinal barrier model and HepG2 as a hepatic cell line. First, the protective effect of dairy samples against the oxidative stress caused by menadione was analyzed. All these dairy fractions significantly reversed the oxidative stress, with the non-washed buttermilk fraction presenting the greatest antioxidant effect for Caco-2 cells and lactoferrin as the best antioxidant for HepG2 cells. At concentrations that did not impact cell viability, we found that the dairy sample with the highest antigenotoxic power against menadione, in both cell lines, was lactoferrin at the lowest concentration. Additionally, dairy by-products maintained their activity in a coculture of Caco-2 and HepG2, mimicking the intestinal-liver axis. This result suggests that the compounds responsible for the antioxidant activity could cross the Caco-2 barrier and reach HepG2 cells on the basal side, exerting their function on them. In conclusion, our results show that dairy by-products have antioxidant and antigenotoxic activities, which would allow revaluing their use in food specialties.

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“…Typhimurium and decreases its infectious inflammation. [39][40][41][42][43][44] After the Lf proteolytic digestion with pepsin, lactoferricin (Lfc), a bioactive peptide, is released from the N-terminal region of Lf that shows higher antimicrobial action than Lf. 12 The Lf and Lfc antimicrobial activity has not been restricted to human pathogenic microbes.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

A comprehensive review on lactoferrin: a natural multifunctional glycoprotein

Shini

Nisha

2022

Food Funct.

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Protective effect of bovine lactoferrin against Cronobacter sakazakii in human intestinal Caco-2/TC7 cells

Cited by 14 publications

References 62 publications

Effect of in vitro gastrointestinal digestion on the antibacterial activity of bioactive dairy formulas supplemented with lactoferrin against Cronobacter sakazakii

Effect of in vitro gastrointestinal digestion on the antibacterial activity of bioactive dairy formulas supplemented with lactoferrin against Cronobacter sakazakii

Dairy By-Products and Lactoferrin Exert Antioxidant and Antigenotoxic Activity on Intestinal and Hepatic Cells

A comprehensive review on lactoferrin: a natural multifunctional glycoprotein

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