Protective Effect of Bovine Milk Whey Protein Concentrate on the Ulcerative Lesions Caused by Subcutaneous Administration of Indomethacin

Abstract

The protective effect of a whey protein concentrate (WPC) was studied in three models of stomach ulcerative lesions induction: subcutaneous injection of indomethacin, and stress induced by either intraperitoneal injection of reserpine, or immobilization and holding in the cold (4 degrees C, 2 hours). Adult Wistar rats (300-400 g) were used for acute (single-dose), repetitive, or subchronic (10 days) administration of WPC prior to treatment with the ulcerogenic factors. The best protection was achieved in the indomethacin model for repetitive and subchronic experiments, reaching 50.1% and 44%, respectively, inhibition of the ulcerative lesions, which was significant at 1% probability (P <.01). For the immobilization and cold model, maximum inhibition by WPC was 22.1%, and that for the reserpine model was 23.8%. In both models the inhibition was not significant (P >.05) compared with saline (negative control).

Antiulcerative properties of bovine α-lactalbumin

“…Studies reported recently (Rosaneli, Bighetti, Antoˆnio, Carvalho, & Sgarbieri, 2002, 2004) with a WPC produced in a pilot plant (Zinsly et al, 2001) and with a Comm.a-La (Matsumoto et al, 2001) demonstrated that whey proteins were capable of inhibiting the development of ulcerative lesions in the rat stomach mucosa by various ulcerogenic agents. Rosaneli et al (2004) used the same WPC, which was used in the present work to isolate a-La, and reported a 50% decrease of the ULI when two consecutive daily doses of 1000 mg kg À1 bw were administered to the rat, in the indomethacin model.…”

“…Rosaneli et al (2004) used the same WPC, which was used in the present work to isolate a-La, and reported a 50% decrease of the ULI when two consecutive daily doses of 1000 mg kg À1 bw were administered to the rat, in the indomethacin model. If one considers a-La as the only active compound in WPC against ulcerative lesions caused by indomethacin and an average concentration of 20% aLa in the WPC, then the 50% lowering in ULI by a 1000 mg dosage of WPC is perfectly coherent with the 12% decrease by 200 mg kg À1 bw of a-La used in the present study.…”

“…Our group (Rosaneli et al, 2002(Rosaneli et al, , 2004 showed the importance of sulfhydryl substances in the protection of Table 2 Effect of a-lactalbumin produced in the laboratory (Lab.a-La), commercial a-lactalbumin (Comm.a-La) and two Comm.a-La hydrolysates (F 1 and F 2 ), and carbenoxolone on the gastric production of mucus a Intragastric intubation Table 3 Effect of a-lactalbumin produced in the laboratory (Lab.a-La), a commercial a-lactalbumin (Comm.a-La) and two Comm.a-La hydrolysates (F 1 and F 2 ), indomethacin, and carbenoxolone on the biosynthesis of prostaglandin (PGE 2 ) in the rat gastric mucosa gastric mucosa against indomethacin and absolute ethanol. The combination of saline plus NEM permitted to estimate the protection offered to the gastric mucosa by naturally occurring sulfhydryl substances.…”

New Approaches in Gastritis Treatment

“…Whey proteins as α-lactalbumin would also have gastroprotector effect (Matsumoto et. al., 2001;Rosaneli et. al., 2004;Ushida et.…”

Partial chemical and functional characterization of milk whey products obtained by different processes

“…Whey proteins have been reported to lower blood cholesterol (JACOBUCCI et al, 2001) and protect gastric mucosa against ulcerogenic drugs (ROSANELI et al, 2002;ROSANELI et al, 2004;MEZZAROBA et al, 2006).…”