Protective Effect of Carvacrol on Renal Functional and Histopathological Changes in Gentamicin-Induced-Nephrotoxicity in Rats

Abstract

Background: Nephrotoxicity is one of the most important side effects of the use of gentamicin sulphate (GS) resulted in reactive oxygen species generation. Antioxidant compounds played effective roles in reduction of renal injuries caused by using of gentamicin. Carvacrol is a strong antioxidant compound. Objectives: The aim of this study is to explore the effect of carvacrol inhibition in lesions of gentamicin-induced nephrotoxicity. Materials and Methods:In this experimental study, 32 male mature Sprague-Dawley rats were divided into 4 groups of 8; group1: control, group 2 sham received daily carvacrol injection (74 mg/kg) for 12 days, group 3 received daily GS injection (100 mg/kg) for 12 days, group 4 received daily GS (100 mg/kg) and carvacrol (74 mg/kg) for 12 days. After 12 days, rats were anaesthetized, blood sample were obtained and kidneys were removed then stained with hematoxylin and eosin method and then were studied histophatologically. Serum creatinine and urea were measured. Results: Flow treatment of nephrotoxic animals with carvacrol could significantly inhibit leukocyte infiltration (9.42%) and tubular necrosis (38.18%) in comparison with the nephrotoxic untreated group. Carvacrol significantly decreased the levels of urea and creatinine in treated group compared with the nephrotoxic untreated group. Conclusions: The findings showed that carvacrol alleviates loss of leukocyte infiltration (9.42%) and tubular necrosis and exerts beneficial effects on kidney function test in nephrotoxic group.

Carvacrol attenuates cyclophosphamide-induced oxidative stress in rat kidney

“…Samarghandian et al (2016) has suggested an ameliorative effect of CAR against immobilization stressed rats showing decreases in kidney SOD, CAT and GSH levels. Ahmadvand et al (2016) reported that CAR has partial protective effect on gentamicin-induced renal toxicity. They showed a significant decrease in serum urea, but not in CRE levels.…”

“…Furthermore, focal interstitial and tubular edema, connective tissue infiltration, cortical tubular vacuolization and glomerular observed in his study. Histology results ofAhmadvand et al (2016) suggested diminished tubular necrosis, but no change in leukocyte infiltration Bozkurt et al (2014). showed that CAR plus methotrexate treated rats exhibited decreased tubular degeneration compared to animals treated with methotrexate alone, but the difference was not statistically significant El-Sayed et al (2015).…”

Nigella sativa oil protects against emamectin benzoate‐Induced neurotoxicity in rats

“…Carvacrol treatment was reported to attenuate the increased levels of urea and creatinine in gentamicin-intoxicated rats. 37 The current results revealed that EMB has a neurotoxic effect.…”

Ameliorative Effect of Berberis Vulgaris Fruit Extract Against Gentamicin Induced Nephrotoxicity in Albino Rats

“…Gentamicin is the most common antibiotic against gramnegative bacterial infections. One of the main side effects of gentamicin is nephrotoxicity 1 . Impairment occurs in 30% of patients treated with gentamicin 2 .…”