Protective Effect of Cyclo(His-Pro) on Streptozotocin-Induced Cytotoxicity and Apoptosis In Vitro

Koo, Kwang Bon; Suh, Hyung Joo; Soo, Kyung; Choi, Jang Won

doi:10.4014/jmb.1012.12003

Cited by 26 publications

(18 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results are consistent with our previous reports that show similar effects with other pentacyclic triterpenes like betulinic, oleonolic, and ursolic acids in yet another mouse model of high-fat fed-induced obesity [28-30]. Excessive production of nitric oxide and the subsequent increase in local oxidative stress, or altered intracellular Ca +2 regulation, are some suggested pathogenic mechanisms in pancreatic beta-cell death and the development of insulin-dependent diabetes mellitus-induced by STZ [31]. Many conventional drugs used to treat diabetes act by improving insulin sensitivity, increasing insulin production, and or by decreasing the level of blood glucose.…”

Section: Discussionsupporting

confidence: 93%

Antihyperglycemic and hypolipidemic effects of α, β-amyrin, a triterpenoid mixture from Protium heptaphyllum in mice

et al. 2012

View full text Add to dashboard Cite

BackgroundPentacyclic triterpenes in general exert beneficial effects in metabolic disorders. This study investigated the effects of α, β-amyrin, a pentacyclic triterpene mixture from the resin of Protium heptaphyllum on blood sugar level and lipid profile in normal and streptozotocin (STZ)-induced diabetic mice, and in mice fed on a high-fat diet (HFD).FindingsMice treated with α, β-amyrin (10, 30 and 100 mg/kg, p.o.) or glibenclamide (10 mg/kg, p.o.) had significantly reduced STZ-induced increases in blood glucose (BG), total cholesterol (TC) and serum triglycerides (TGs). Unlike glibenclamide that showed significant reductions in BG, TC and TGs in normoglycemic mice, α, β-amyrin did not lower normal blood sugar levels but at 100 mg/kg, manifested a hypolipidemic effect. Also, α, β-amyrin effectively reduced the elevated plasma glucose levels during the oral glucose tolerance test. Moreover, the plasma insulin level and histopathological analysis of pancreas revealed the beneficial effect of α, β-amyrin in the preservation of beta cell integrity. In mice treated orally with α, β-amyrin (10, 30 and 100 mg/kg) or fenofibrate (200 mg/kg), the HFD-associated rise in serum TC and TGs were significantly less. The hypocholesterolemic effect of α, β-amyrin appeared more prominent at 100 mg/kg with significant decreases in VLDL and LDL cholesterol and an elevation of HDL cholesterol. Besides, the atherogenic index was significantly reduced by α, β-amyrin.ConclusionsThese findings reflect the potential antihyperglycemic and hypolipidemic effects of α, β-amyrin mixture and suggest that it could be a lead compound for drug development effective in diabetes and atherosclerosis.

show abstract

Section: Discussionsupporting

confidence: 93%

Antihyperglycemic and hypolipidemic effects of α, β-amyrin, a triterpenoid mixture from Protium heptaphyllum in mice

et al. 2012

View full text Add to dashboard Cite

show abstract

“…The sample was centrifuged (12,000xg) in an Eppendorf centrifuge for 3 min, and the supernatants were filtered through a 0.22 µm membrane filter. The CHP content was measured as described previously [32].…”

Section: Methodsmentioning

confidence: 99%

Alterations in Pancreatic Protein Expression in STZ-Induced Diabetic Rats and Genetically Diabetic Mice in Response to Treatment with Hypoglycemic Dipeptide Cyclo (His-Pro)

Park

Choi²,

Yun

et al. 2012

Cell Physiol Biochem

View full text Add to dashboard Cite

To provide insights into the molecular mechanisms underlying diabetes mellitus, we performed a proteomic study on two diabetic animal models, streptozotocin (STZ)-induced diabetic rats (T1DM) and genetically diabetic (C57BL/6J ob/ob) mice (T2DM). To better understand the recovery process of those diabetic rodents, we examined the effect of hypoglycemic dipeptide Cyclo (His-Pro) (CHP) treatment on the differential expression of pancreatic proteins in both animal models. Oral administration of CHP had an excellent hypoglycemic effect in both animal models, lowering the average plasma glucose level by over 50%. Pancreatic proteins were separated by two-dimensional gel electrophoresis (2-DE) and identified by MALDI-TOF mass spectrometry. This study allowed, for the first time, the identification of 34 proteins that are related to diabetes and potential targets of CHP, a potent anti-diabetic agent for both T1DM and T2DM. The alterations in the expression of these proteins could indicate a tendency for diabetic animals to overcome their diabetic state. These proteins are involved in cellular functions such as metabolism, cellular structure, oxidative stress, as well as signal and energy transduction. Some have already been linked to diabetes, suggesting that the newly identified proteins might also be significant in the etiology of this pathology and should be further investigated. Furthermore, CHP has emerged as a potent tool for both the treatment and study of the molecular mechanisms underlying diabetes. Thus, the findings presented here provide new insights into the study and potential treatment of this pathology.

show abstract

“…For example, TRH is also known to play a role in regulating mood (Sun et al, 2010), arousal (Hara et al, 2009), cognition (Molchan et al, 1990), anxiety (Gutierrez-Mariscal et al, 2008), and motor coordination (Pitts et al, 1995; Kinoshita et al, 1998). Additionally, some of the peripheral effects of TRH and its analogs include the ability to increase insulin absorption in the pancreas (Koo et al, 2011), regulation of motility (Varanasi et al, 2002) and acid secretion (Tache et al, 1980) in the gastrointestinal tract, ionotropic support in the heart (Jin et al, 2004), and regulation of hair growth (Gaspar et al, 2010). TRH has also been demonstrated to exert trophic actions in lower motor neurons of the spinal cord (Van den Bergh et al, 1991), and pro-TRH gene expression has been shown to play a role in preventing neuronal apoptosis in the CNS.…”

Section: Introductionmentioning

confidence: 99%

The role of Thyrotropin Releasing Hormone in aging and neurodegenerative diseases

Daimon¹,

Chirdon²,

Maudsley³

et al. 2013

AJAD

View full text Add to dashboard Cite

Thyrotropin releasing hormone (TRH) is primarily known as the central regulator of the hypothalamic-pituitary-thyroid (HPT) axis. However, TRH also exerts a variety of central nervous system effects independent from its activity in the HPT axis. With advancing age, decreases in TRH synthesis, expression, and activity have been demonstrated. Associated with this emerging evidence suggests that TRH is implicated in neurodegenerative diseases of aging, including Alzheimer’s disease and Parkinson’s disease. TRH and its synthetic analogs have been recognized as trophic factors in neurons of the diencephalon and spinal cord, and as neuroprotectants against oxidative stress, glutamate toxicity, caspase-induced cell death, DNA fragmentation, and inflammation. In this review, we will provide an overview of some of the roles of TRH, outside of the HPT axis, associated with pathological aging and neurodegeneration and we shall discuss the potential of TRH and TRH analogs for the treatment of neurodegenerative diseases.

show abstract

Protective Effect of Cyclo(His-Pro) on Streptozotocin-Induced Cytotoxicity and Apoptosis In Vitro

Cited by 26 publications

References 62 publications

Antihyperglycemic and hypolipidemic effects of α, β-amyrin, a triterpenoid mixture from Protium heptaphyllum in mice

Antihyperglycemic and hypolipidemic effects of α, β-amyrin, a triterpenoid mixture from Protium heptaphyllum in mice

Alterations in Pancreatic Protein Expression in STZ-Induced Diabetic Rats and Genetically Diabetic Mice in Response to Treatment with Hypoglycemic Dipeptide Cyclo (His-Pro)

The role of Thyrotropin Releasing Hormone in aging and neurodegenerative diseases

Contact Info

Product

Resources

About