Protective effect of dietary curcumin in Anabas testudineus (Bloch) with a special note on DNA fragmentation assay on hepatocytes and micronucleus assay on erythrocytes in vivo

Abstract

The present study was conducted to evaluate the safety of long-term dietary curcumin at doses 0.5 and 1% in Anabas testudineus employing hematological and cytological techniques. The fish were fed with curcumin-supplemented feed for 6 months. Fine blood smears were prepared and subjected to three different staining techniques. The erythrocyte micronucleus frequency (MN) and the cytometric measurements of erythrocytes were determined. Blood from the control and treated fish was subjected to the assessment of several hematological parameters. Also, DNA fragmentation assay on hepatocytes was conducted. The results showed that hemoglobin content, RBC count and hematocrit increased in the curcumin-fed fish compared to control, whereas WBC count, platelet count, mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration were unaffected. WBC/RBC ratio was lower in the case of curcumin-treated fish. The cytometric measurements revealed no change in the erythrocytes and their nuclei after curcumin treatment. DNA fragmentation assay revealed intact DNA in curcumin-fed group, ruling out the possibility of curcumin-induced DNA damage. The positive control group showed a significant increase in MN frequency compared to negative control and curcumin-fed groups. In fact, the MN frequency decreased in 1% curcumin-fed group compared to the negative control and 0.5% curcumin groups. All these indicated a state of well-being of the curcumin-treated fish. Therefore, it is concluded that curcumin could be used as a safe feed ingredient to improve the growth of finfish in aquaculture.

The Possible Ameliorative Effect of Curcumin on Atrazine-Induced Oxidative Stress, DNA Damage, Mitochondrial Dysfunction, and Apoptosis in the Kidney of Adult Male Albino Rats

“…This could be attributed to the ability of ATR to attack hydroxyl radicals on DNA which results in single or double strand breaks (Dizdaroglu, 1992). Curcumin supplementation in the current study caused significant decrease in plasma and urine 8-OHdG levels, the results were in harmony with Manju, et al, (2013) who reported the genoprotective effect of curcumin.…”

Immuomodulatory effect of dietary turmeric supplementation on Nile tilapia (Oreochromis niloticus)

“…These data to some extent coincide with those reported by Sivagurunathan, Amilameera, and Innocent () who reported no effect of TUM on TLC in fish with significant ( p < 0.05) elevation in lymphocyte counts. However, Manju, Vijayasree, Akbarsha, and Oommen () observed no effect of TUM on fish leucocytes numbers. The higher TLC and lymphocyte count in T2 than in control may be due to the stress reducing effect of TUM (D'souza & Prabhu, ) that maintained a healthy numbers of the immune cells that was reflected positively on fish performance.…”

Combined antiparasitic and anti‐inflammatory effects of the natural polyphenol curcumin on turbot scuticociliatosis

“…), and the compound has shown positive effects on growth of fish in aquaculture (Manju, Akbarsha & Oommen ; Manju et al . ; Mahfouz ).…”

“…Furthermore, CUR has been reported to inhibit the host NFjB signalling pathway, which is activated during several parasite infections and interferes in inflammation and oxidative stress (Haddad, Sauvain & Deharo 2011;Cao et al 2015). The safety profile of CUR has so far been found to be excellent (Clarke & Mullin 2008;He et al 2015), and the compound has shown positive effects on growth of fish in aquaculture (Manju, Akbarsha & Oommen 2012;Manju et al 2013;Mahfouz 2015).…”