Protective effect of dietary perilla oil on allergic inflammation in asthmatic mice

Abstract

Perilla oil (PER) is rich in α‐linolenic acid (n‐3 fatty acid). To unravel the effects of dietary PER on allergic asthmatic inflammation, three kinds of dietary oil, including PER, corn oil (COR), and perilla compound oil (50% PER and 50% COR), were used for replacing the oil in an AIN76 feed consumed by ovalbumin (OVA)‐sensitized and challenged mice continuously for 5 wk. T‐helper type 1 lymphocyte (Th1)/T‐helper type 2 lymphocyte (Th2) and pro‐/anti‐inflammatory cytokines secreted by the cells from the airway, the lungs, and the spleen of experimental mice were determined by ELISA. The results showed that dietary PER inhibited interleukin (IL)‐1β and tumor necrosis factor (TNF)‐α secretions by lipopolysaccharide (LPS)‐stimulated lung cells, as well as interferon (IFN)‐γ and IL‐6 secretions by LPS‐stimulated splenocytes. Perilla compound oil increased the secretion ratio of IFN‐γ/IL‐5 (Th1/Th2 cytokines) in LPS‐stimulated bronchoalveolar lavage fluid cells, but decreased the ratio of IL‐6/IL‐10 (pro‐/anti‐inflammatory cytokines) in LPS‐stimulated splenocytes. The present study demonstrated that dietary PER and its compound oil protected the airways, the lungs, and the spleen from allergic inflammation in OVA‐challenged asthmatic mice, suggesting that an appropriate n‐6/n‐3 fatty acid ratio at a ratio of 1:1 or less in dietary oil may be beneficial to improve the Th2‐skewed allergic asthmatic inflammation. Practical applications: The present study demonstrated that dietary PER and its compound oil protected the airways, the lungs, and the spleen from allergic inflammation in OVA‐challenged asthmatic mice, suggesting that an appropriate n‐6/n‐3 fatty acid ratio at a ratio of 1:1 or less in dietary oil may be beneficial to improve the Th2‐skewed allergic asthmatic inflammation.

Perilla Oil Alleviates High-Fat Diet-Induced Inflammation in the Colon of Mice by Suppressing Nuclear Factor-Kappa B Activation

“…12,14,15 It is also reported to improve allergic inflammation in mice. 16 Compared with the extensive use of PO used for edible purposes, its impact on various health aspects has not yet thoroughly analyzed. Furthermore, most of the studies related to polyunsaturated fatty acid (PUFA) have focused on FO, which is used as an omega-3 fatty acid supplement.…”

Menthone Inhalation Alleviates Local and Systemic Allergic Inflammation in Ovalbumin-Sensitized and Challenged Asthmatic Mice

“…The OVA-sensitized and challenged allergic asthmatic inflammation mouse model was established using OVA sensitization injected intraperitoneally (i. p .) and challenged with aerosolized-OVA to induce stronger airway inflammation [ 32 , 33 , 34 , 35 , 36 , 37 ]. Briefly, the experiment mice in all groups, except the NSC group, were sensitized i .…”

“…Immediately after BALF was harvested, the peritoneal macrophages of the experiment mice were isolated and performed as previously described [ 35 , 36 , 38 , 39 ]. Briefly, the isolated peritoneal macrophages (>95%) from each experiment mouse were adjusted to the cell density of 2 × 10 6 cells/mL TCM medium for culture.…”

The anti‐tussive, anti‐inflammatory effects and sub‐chronic toxicological evaluation of perilla seed oil

“…Ingestion of a PUFA or its higher derivatives like fish oil have been shown to suppress the synthesis of the n‐6 series of leukotrienes by competing and inhibiting the metabolism of the arachidonic acid, which exhibited unique and essential roles in an allergic response 10,11 . As the best resources for additional PUFAs, the PSO also has been known to possess great potential in the regulation of lung function 5,12 . Considering the lack of studies addressing the biological potential and scientific information of PSO, the present study aims to assess the pharmacological activities and the toxicological profile of PSO.…”

“…10,11 As the best resources for additional PUFAs, the PSO also has been known to possess great potential in the regulation of lung function. 5,12 Considering the lack of studies addressing the biological potential and scientific information of PSO, the present study aims to assess the pharmacological activities and the toxicological profile of PSO.…”