Protective effect of dried safflower petal aqueous extract and its main constituent, carthamus yellow, against lipopolysaccharide-induced inflammation in RAW264.7 macrophages

Wang, Ching Chiung; Choy, Cheuk-Sing; Liu, Yung Hung; Cheah, Khoot Peng; Li, Joe Sharg; Wang, Jimmy Tse Jen; Yu, Wen Yu; Lin, Che-Wei; Cheng, Hui; Hu, Chien-Ming

doi:10.1002/jsfa.4172

Cited by 60 publications

(33 citation statements)

References 34 publications

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“…It is a natural, edible pigment approved in China and as a state-level new drug. Recently, Chinese and foreign scholars have conducted extensive and in-depth studies on the extraction and pharmacological effects of SY Xi et al, 2012), and found that SY is not only a valuable natural edible pigment, with the advantages of attractive color, resistance to high temperatures, high pressure, low temperature, light, acid, and reduction, and antimicrobial properties, but also has many pharmacological properties such as expansion of coronary arteries, antioxidation, myocardial protection, lowering of blood pressure, immune suppression, and cerebral protection (Wang et al, 2011;Chen et al, 2012). In this study, we examined the effect of SY on the blood lipids of hyperlipidemic mice and the in vitro synthesis of cholesterol to provide a basis for its pharmacological applications.…”

Section: Introductionmentioning

confidence: 99%

Hypolipidemic effect of safflower yellow and primary mechanism analysis

Wang¹,

Ren²,

Ma³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. We examined the hypolipidemic effect of safflower yellow (SY) on hyperlipidemic mice and its influence on the biological synthesis of cholesterol in cells. Over 4 weeks, the levels of total cholesterol, triglyceride, low-density lipoprotein cholesterol, and highdensity lipoprotein cholesterol in serum were detected using a kit; mouse liver samples were acquired for paraffin sections, and mouse liver cells were observed under light microscope. Chinese hamster ovary cells were cultured in vitro, and an amphotericin B-cell model was adopted to observe the inhibitory effect of SY on the biological synthesis of intracellular cholesterol. An enzyme-linked immunosorbent assay was used to detect the survival rate of Chinese hamster ovary cells. The middle and high doses of SY significantly reduced the levels of total cholesterol, triglycerides, and low-density lipoprotein cholesterol in the serum of hyperlipidemic mice and low-density lipoprotein cholesterol/ high-density lipoprotein cholesterol ratio (P < 0.05), and the fatty liver of hyperlipidemic mice was significantly alleviated. SY had a protective 6271 Hypolipidemic effect of safflower yellow ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 6270-6278 (2015) effect on Chinese hamster ovary cells following amphotericin B injury (P < 0.01). SY exerts significant hypolipidemic effects and prevents fatty liver in a mechanism associated with inhibition of the biosynthesis of intracellular cholesterol.

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Section: Introductionmentioning

confidence: 99%

Hypolipidemic effect of safflower yellow and primary mechanism analysis

Wang¹,

Ren²,

Ma³

et al. 2015

Genet. Mol. Res.

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“…However, after exposure to a stimuli, such as LPS, IκBα phosphorylation increases, which triggers its proteolytic degradation, leading to NF-κB activation and the expression of genes that play important roles in innate and adaptive immunity. 43,44 Interestingly, hBD3-3 blocks phosphorylation-induced IκBα degradation, thereby inhibiting the nuclear migration of NF-κB p65 subunits ( Figure 6). Based on these results, we suggest that hBD3-3 possesses outstanding anti-inflammatory activity through its targeting of canonical NF-κB pathway-mediated inflammation in cells.…”

mentioning

confidence: 98%

Identification of a cell-penetrating peptide domain from human beta-defensin 3 and characterization of its anti-inflammatory activity

Lee¹,

Suh²,

Kim³

et al. 2015

IJN

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Human beta-defensins (hBDs) are crucial factors of intrinsic immunity that function in the immunologic response to a variety of invading enveloped viruses, bacteria, and fungi. hBDs can cause membrane depolarization and cell lysis due to their highly cationic nature. These molecules participate in antimicrobial defenses and the control of adaptive and innate immunity in every mammalian species and are produced by various cell types. The C-terminal 15-mer peptide within hBD3, designated as hBD3-3, was selected for study due to its cell- and skin-penetrating activity, which can induce anti-inflammatory activity in lipopolysaccharide-treated RAW 264.7 macrophages. hBD3-3 penetrated both the outer membrane of the cells and mouse skin within a short treatment period. Two other peptide fragments showed poorer penetration activity compared to hBD3-3. hBD3-3 inhibited the lipopolysaccharide-induced production of inducible nitric oxide synthase, nitric oxide, and secretory cytokines, such as interleukin-6 and tumor necrosis factor in a concentration-dependent manner. Moreover, hBD3-3 reduced the interstitial infiltration of polymorphonuclear leukocytes in a lung inflammation model. Further investigation also revealed that hBD3-3 downregulated nuclear factor kappa B-dependent inflammation by directly suppressing the degradation of phosphorylated-IκBα and by downregulating active nuclear factor kappa B p65. Our findings indicate that hBD3-3 may be conjugated with drugs of interest to ensure their proper translocation to sites, such as the cytoplasm or nucleus, as hBD3-3 has the ability to be used as a carrier, and suggest a potential approach to effectively treat inflammatory diseases.

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“…The reactive free radical NO, which is synthesized by iNOS, is a major macrophage-derived inflammatory mediator and has also been reported to be involved in the development of inflammatory diseases. PGE 2 is an other import anti-inflammatory mediator and is produced from arachidonicacid metabolites by the catalysis of Cyclooxygenase-2 (COX-2) [21]. Thus, we investigated the anti-inflammatory activities of E. cava enzymatic extract and AG-DK on LPS-induced NO and PGE 2 production in murine macrophage RAW 264.7 cells.…”

Section: Resultsmentioning

confidence: 99%

Biological Potential of Enzymatic and Polyphenol Extracts from Ecklonia cava

Lee¹,

Kim²,

Oh³

et al. 2013

Journal of the Society of Cosmetic Scientists of Korea

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To investigate the efficacy of enzymatic extract of Ecklonia cava and its polyphenol extract (AG-DK) as cosmetic ingredients, the anti-oxidative effect, anti-glycation effect, anti-melanogenic effect, and anti-inflammatory effect of the extracts were evaluated in vitro. The enzymatic extract of E. cava (SC50 42.9 ppm) and AG-DK (SC50 6.4 ppm) showed a strong DPPH free radical scavenging activity. The anti-glycation ability of the enzymatic extract of E. cava and AG-DK was tested using bovine serum albumin (BSA), which inhibited the formation of advanced glycation end-products (AGEs) in the BSA/glucose system. The enzymatic extract of E. cava (IC50 97.2 ppm) and AG-DK (IC50 7 ppm) had inhibitory effects on tyrosinase activity. Moreover, the enzymatic extract of E. cava and AG-DK had an anti-inflammatory effect through the inhibition of nitricoxide (NO) and prostaglandin E2 (PGE2). These findings suggest that the enzymatic extract of E. cava and AG-DK can be applied to skin-care products as cosmetic ingredients.

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Protective effect of dried safflower petal aqueous extract and its main constituent, carthamus yellow, against lipopolysaccharide-induced inflammation in RAW264.7 macrophages

Cited by 60 publications

References 34 publications

Hypolipidemic effect of safflower yellow and primary mechanism analysis

Hypolipidemic effect of safflower yellow and primary mechanism analysis

Identification of a cell-penetrating peptide domain from human beta-defensin 3 and characterization of its anti-inflammatory activity

Biological Potential of Enzymatic and Polyphenol Extracts from Ecklonia cava

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