Protective effect of fustin against adjuvant-induced arthritis through the restoration of proinflammatory response and oxidative stress

Abstract

Rheumatoid arthritis causes irreparable damage to joints. The present research sought to check fustin’s anti-arthritic efficacy against the complete Freund’s adjuvant-induced arthritis paradigm in animals by altering the inflammatory response. In the rats, complete Freund’s adjuvant was used to trigger arthritis and they received fustin at 50 and 100 mg/kg for 21 days. At regular intervals, the hind paw volume and arthritic score were assessed. After the trial period, hematological, antioxidant, pro-inflammatory cytokines, and other biochemical parameters were estimated. Fustin-treated rats showed the down-regulation of hind paw volume, arthritic score, and altered hematological parameters (TLC, DLC (neutrophil, lymphocyte, monocyte, eosinophil, basophil)). Furthermore, fustin significantly mitigates proinflammatory cytokine (reduced interleukin, tumor necrosis factor-a (TNF-α), IL-6, IL-1β), oxidative stress (attenuated malondialdehyde (MDA), catalase (CAT), glutathione (GSH), superoxide dismutase (SOD)), attenuated production of prostaglandin E2 and myeloperoxidase (MPO) and improved nuclear factor erythroid 2-related factor (Nrf2) action. Fustin led to the benefit in arthritis-prone animals elicited by complete Freund’s adjuvant via pro-inflammatory cytokine.

WITHDRAWN: Effects of ethyl acetate fraction ofPsychotria vogelianaleaf on liver function, kidney function, and cytokines level of induced rheumatoid arthritis in albino rats

“…This correlates with previous findings 31 . Elevated levels of ALP, AST, and ALT in the blood biochemical findings of the CFA-induced rheumatoid arthritis rat model indicate impairment of the liver, and this is also related to the inflammatory situation 32 . This is also in consonance with the study of 33 .…”

Anti-Rheumatoid Arthritis Activity of 96% Ethanol Extract of Eleutherine bulbosa Bulbs with Arthritis Induction Adjuvant Method

“…With the AIA method, CFA induction closely resembled human rheumatoid disease and was commonly utilized to assess inflammatory illness. It was reliable as a model for persistent pain and an experimental model of CFA-induced polyarthritis in animal tests 27 . The arthritis The positive group was given methylprednisolone 15 mg/KgBW because it belongs to the class of corticosteroids that can treat RA.…”

Current Knowledge of the Health-promoting Effects of Fustin – a Mini-review

“…Clinical trials were scarce and anti-fibrotic effect was the only pharmacological activity documented in one human study [5]. These effects were ascribed to the antioxidant and antiinflammatory activities of the flavonoid [6]. Additional studies are required to investigate the health-beneficial effects of fustin both in experimental and clinical settings.…”