Protective effect of glutathione on the cytotoxicity caused by a combination of aluminum and iron in suspension‐cultured tobacco cells

Yamaguchi, Yukiko; Yamamoto, Yoko; Ikegawa, Hiroshi; Matsumoto, Hideaki

doi:10.1034/j.1399-3054.1999.105305.x

Cited by 14 publications

(9 citation statements)

References 34 publications

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“…Recently, Devi et al (2003) reported that the higher content of AsA in Al tolerant cell line of tobacco than in Al sensitive cell line are responsible for its higher tolerance to Al. Yamaguchi et al (1999) observed that total GSH concentration in tobacco cell suspension treated with a combination of Al and iron was lower than in the control cells. High content of GSH has been shown to be responsible for the tolerance mechanism of tobacco cells (Devi et al 2003) and wheat plant (Dong et al 2002) to Al.…”

Section: Introductionmentioning

confidence: 66%

“…It has been shown that high content of AsA is responsible for tolerance mechanism of tobacco cells to Al by protecting cells from lipid peroxidation (Devi et al 2003) and GSH is able to protect cells from either peroxidation or H 2 O 2 commonly enhanced by Al (Devi et al 2003, Dong et al 2002, Yamaguchi et al 1999). However, AlCl 3 treatment did not increase lipid peroxidation and decreased H 2 O 2 content in rice roots (Fig.…”

Section: Discussionmentioning

confidence: 97%

“…It has been shown that Al induces reactive oxygen species (ROS) and enhances lipid peroxidation in Hordeum vulgare (Sakihama andYamasaki 2002, Šimonovicová et al 2004a,b), Oryza sativa (Kuo andKao 2003, Meriga et al 2004), Pisum sativum (Yamamoto et al 2001(Yamamoto et al , 2002, Triticum aestivum (Darkó et al 2004), and Zea mays (Boscolo et al 2003). In Arabidopsis and cultured tobacco cells, Al induced the expression of several genes (e.g.…”

Section: Introductionmentioning

confidence: 98%

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Protective effect of ascorbic acid and glutathione on AlCl<sub>3</sub>-inhibited growth of rice roots

Wang¹,

Kao²

2007

Biologia plant.

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The effect of AlCl 3 on the antioxidant system of rice roots and the role of applied antioxidants ascorbic acid (AsA) and glutathione (GSH) in AlCl 3 -inhibited growth of rice roots were investigated. AlCl 3 treatment resulted in a rapid inhibition of root growth but had no effect on lipid peroxidation and antioxidative enzyme activities in rice roots. AlCl 3 treatment resulted in lower content of H 2 O 2 , AsA, and GSH than in controls. Exogenous AsA or GSH counteracted growth inhibition of rice roots induced by AlCl 3 . AlCl 3 treatment increased syringaldazine peroxidase (SPOX) activities and lignin content in rice roots. Exogenous AsA or GSH prevented the decrease in H 2 O 2 content and the increase in SPOX activities and lignin content in rice roots caused by AlCl 3 . Results suggest that lignification induced by low AsA or GSH content may explain the mechanism of Al-inhibited growth of rice roots.

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Section: Introductionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 98%

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Protective effect of ascorbic acid and glutathione on AlCl<sub>3</sub>-inhibited growth of rice roots

Wang¹,

Kao²

2007

Biologia plant.

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“…Iron excess leads to cytotoxic effects. This effect is enhanced by the presence of other metal ions, such as aluminium [81]. It seems that glutathione plays an important protective role in cytotoxicity induced by iron ions.…”

Section: Resultsmentioning

confidence: 99%

Effect of Magnetic Nanoparticles on Tobacco BY-2 Cell Suspension Culture

Kryštofová

Sochor

Zítka

et al. 2012

IJERPH

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Nanomaterials are structures whose exceptionality is based on their large surface, which is closely connected with reactivity and modification possibilities. Due to these properties nanomaterials are used in textile industry (antibacterial textiles with silver nanoparticles), electronics (high-resolution imaging, logical circuits on the molecular level) and medicine. Medicine represents one of the most important fields of application of nanomaterials. They are investigated in connection with targeted therapy (infectious diseases, malignant diseases) or imaging (contrast agents). Nanomaterials including nanoparticles have a great application potential in the targeted transport of pharmaceuticals. However, there are some negative properties of nanoparticles, which must be carefully solved, as hydrophobic properties leading to instability in aqueous environment, and especially their possible toxicity. Data about toxicity of nanomaterials are still scarce. Due to this fact, in this work we focused on studying of the effect of magnetic nanoparticles (NPs) and modified magnetic nanoparticles (MNPs) on tobacco BY-2 plant cell suspension culture. We aimed at examining the effect of NPs and MNPs on growth, proteosynthesis—total protein content, thiols—reduced (GSH) and oxidized (GSSG) glutathione, phytochelatins PC2-5, glutathione S-transferase (GST) activity and antioxidant activity of BY-2 cells. Whereas the effect of NPs and MNPs on growth of cell suspension culture was only moderate, significant changes were detected in all other biochemical parameters. Significant changes in protein content, phytochelatins levels and GST activity were observed in BY-2 cells treated with MNPs nanoparticles treatment. Changes were also clearly evident in the case of application of NPs. Our results demonstrate the ability of MNPs to negatively affect metabolism and induce biosynthesis of protective compounds in a plant cell model represented by BY-2 cell suspension culture. The obtained results are discussed, especially in connection with already published data. Possible mechanisms of NPs’ and MNPs’ toxicity are introduced.

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“…The TTC method is based on the ability of mitochondria to reduce TTC to formazon by mitochondrial respiration in living cells (Engelmann, 1991). On the other hand, uptake of Evans blue is an indicator of the loss of the plasma membrane integrity as Evans blue is a non-permeating dye in living cells (Yamaguchi et al, 1999). Hence, the decrease in Evans blue uptake by rice suspension cells in the presence of low concentration of NaHS (0.5, 1, 2 mM) may be therefore connected with a decrease in cell membrane permeability.…”

Section: Discussionmentioning

confidence: 99%

Effects of Hydrogen Sulfide on Alternative Pathway Respiration and Induction of Alternative Oxidase Gene Expression in Rice Suspension Cells

Xiao

et al. 2010

Zeitschrift Für Naturforschung C

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The toxic effects of H 2 S on plants are well documented. However, the molecular mechanisms reponsible for inhibition of plants by H 2 S are still not completely understood. We determined the effects of NaHS in the range of 0.5 -10 mM on the growth of rice suspension culture cells, as well as on the expression of the alternative oxidase (AOX) gene. AOX is the terminal oxidase of the alternative pathway (AP) and exists in plant mitochondria. The results showed that H 2 S treatment enhanced the AP activity. During the process of H 2 S treatment for 4 h, the AP activity increased dramatically and achieved the peak value at a concentration of 2 mM NaHS. Then it declined at higher concentrations of NaHS (5 -10 mM) and maintained a steady level. The AOX1 gene transcript level also showed a similar change as the AP activity. Interestingly, different NaHS concentrations seemed to have different effects on the expression of AOX1a, AOX1b, and AOX1c. The induction of AOX expression by low concentrations of NaHS was inferred through a reactive oxygen species (ROS)-independent pathway. At the same time, rice cells grown in culture were very sensitive to H 2 S, different H 2 S concentrations induced an increase in the cell viability. These results indicate that the H 2 S-induced AOX induction might play a role in inhibiting the ROS production and have an infl uence on cell viability.

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Protective effect of glutathione on the cytotoxicity caused by a combination of aluminum and iron in suspension‐cultured tobacco cells

Cited by 14 publications

References 34 publications

Protective effect of ascorbic acid and glutathione on AlCl<sub>3</sub>-inhibited growth of rice roots

Protective effect of ascorbic acid and glutathione on AlCl<sub>3</sub>-inhibited growth of rice roots

Effect of Magnetic Nanoparticles on Tobacco BY-2 Cell Suspension Culture

Effects of Hydrogen Sulfide on Alternative Pathway Respiration and Induction of Alternative Oxidase Gene Expression in Rice Suspension Cells

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