Protective Effect of Grape Seed Proanthocyanidins on Oxidative Damage of Chicken Follicular Granulosa Cells by Inhibiting FoxO1-Mediated Autophagy

Abstract

A significant decrease in poultry egg production occurs due to ovarian aging and autophagy is one of the important factors of ovarian aging that is induced predominantly by oxidative stress. Increasing evidence showed potential roles of plant-derived grape seed proanthocyanidin (GSPs) in protecting ovarian granulosa cells (GCs) from oxidative damage, although the underlying mechanism is still unclear. Here we investigated the possible functions of autophagy involved in the preventive effect of GSPs on oxidative stress in the GCs of ovarian hierarchical follicles of laying chickens. The results showed that increased autophagy was observed in the aging hens (580-day-old, D580) compared with the peak-lay hens (D280). Treatment of GSPs significantly restored the elevated autophagy and decreased viability of cultured D280 chicken GCs that were elicited by hydrogen peroxide. GSPs also suppressed the increased autophagy in the natural aging hens. Similar to the effect of GSPs on GC viability, inhibition of autophagy also showed a protective effect on the decreased viability of GCs under oxidative damage. However, GSPs were not able to provide further protection in GCs that were pretreated with 3-methyladenine (an autophagy inhibitor). In addition to its promoting action on antioxidant capacity, treatment with GSPs increased survival of GCs from autophagy that was caused by oxidative stress through the FoxO1-related pathway. Inhibition of FoxO1 or activation of PI3K-Akt pathway by GSPs increased the confrontation of GCs to oxidative damage and decreased autophagy in GCs. In addition, activation of the SIRT1 signal inhibited the GCs autophagy that was caused by oxidative stress via GSPs-induced deacetylation of FoxO1. These results revealed a new mechanism of GSPs against oxidative stress of GCs via inhibiting FoxO1, which was probably a possible target for alleviating ovarian aging in laying poultry.

Protective effect of rutin on ferroptosis‐induced oxidative stress in aging laying hens through Nrf2/HO‐1 signaling

“…Deparaffinized and rehydrated SWF sections were first processed for antigen retrieval in a 10 mM sodium citrate solution for 20 min at 100°C before BrdU detection. Immunofluorescence staining was performed using standard protocols (Zhou et al, 2022).…”

Allantoin Derived From Dioscorea opposita Thunb Ameliorates Cyclophosphamide-Induced Premature Ovarian Failure in Female Rats by Attenuating Apoptosis, Autophagy and Pyroptosis

“…Large numbers of studies showed that autophagy maintains the energy balance and function of cells and controls the fate of cells through different crosstalk signals, which may have an important impact on female reproductive function [ 25 ]. However, the regulation of the molecular mechanism between apoptosis and autophagy of granulosa cells has not been described fully, and the natural protective agent of granulosa cells on the target of programmed cell death (PCD) has been given a lot of attention [ 26 , 27 ].…”

The role of Procyanidins in delaying the Premature Ovarian Insufficiency through regulatory Sirt1-p53-p21 signaling Pathway in Female Germline Stem Cells

“…Due to their natural antioxidant properties, PACs have been suggested as a possible protector against oxidative stress damage to various organs and tissues, including the ovaries [14][15][16] . Research has shown that PACs can effectively mitigate pathological changes in granulosa cells by inhibiting autophagy and apoptosis, which ultimately contributes towards delaying ovarian aging [17][18][19] . However, studies investigating the effects of PACs on FGSCs remains unreported.…”