Protective effect of hydrogen‐rich saline against radiation‐induced immune dysfunction

Zhao, Sanhu; Yang, Yue; Liu, Wen; Xuan, Zhiqiang; Wu, Shouming; Yu, Shunfei; Mei, Ke; Huang, Yijuan; Zhang, Pei; Cai, Jianming; Jin, Ning; Zhao, Yongxin

doi:10.1111/jcmm.12245

Cited by 37 publications

(32 citation statements)

References 23 publications

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“…The immune system is one of the most important defense mechanisms against IR ( Zhao et al, 2014 ). The damage degree of normal tissue in radiation is dependent on the dose, tissue sensitivity, repair capacity, and prevailing endogenous antioxidant defenses.…”

Section: Discussionmentioning

confidence: 99%

Pine polyphenols fromPinus koraiensisprevent injuries induced by gamma radiation in mice

Wang

Xu³

et al. 2016

PeerJ

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Pine polyphenols (PPs) are bioactive dietary constituents that enhance health and help prevent diseases through antioxidants. Antioxidants reduce the level of oxidative damages caused by ionizing radiation (IR). The main purpose of this paper is to study the protective effect of PPs on peripheral blood, liver and spleen injuries in mice induced by IR. ICR (Institute of Cancer Research) male mice were administered orally with PPs (200 mg/kg b.wt.) once daily for 14 consecutive days prior to 7 Gy γ-radiations. PPs showed strong antioxidant activities. PPs significantly increased white blood cells, red blood cells and platelets counts. PPs also significantly reduced lipid peroxidation and increased the activities of superoxide dismutase, catalase and glutathione peroxidases, and the level of glutathione. PPs reduced the spleen morphologic injury. In addition, PPs inhibited mitochondria-dependent apoptosis pathways in splenocytes induced by IR. These results indicate that PPs are radioprotective promising reagents.

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Section: Discussionmentioning

confidence: 99%

Pine polyphenols fromPinus koraiensisprevent injuries induced by gamma radiation in mice

Wang

Xu³

et al. 2016

PeerJ

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“…Radiation-induced Th1/Th2 imbalance also accounts for immunosuppression in irradiated animals and human [32]. Even after a long time, when the structure of immune organs is reconstructed, immunity remains suppressed [7].…”

mentioning

confidence: 99%

Radioprotective Effects of Heat-Killed Mycobacterium Tuberculosis in Cultured Cells and Radiosensitive Tissues

Chen

et al. 2016

Cell Physiol Biochem

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Background: Exposure to ionizing radiation (IR) often causes severe damage to radiosensitive tissues, which limits the use of radiotherapy in cancer patients. Novel safe and effective radioprotectant is urgently required. It has been reported toll like receptor 2 (TLR2) plays a critical role in radioresistance. In this study, we demonstrated the protective effects of Heat-Killed Mycobacterium tuberculosis (HKMT), a potent TLR2 agonist, against IR. Methods: Cell survival and apoptosis were determined by CCK-8 assay and Annexin V assay, respectively. An immunofluorescence staining assay was used to detect the translocation of nuclear faktor-kappa beta (NF-kB) p65. Tissue damage was evaluated by Haematoxilin-Eosin (HE) staining assay. We also used a flow cytometry assay to measure the number of nucleated cells and CD34+ hemopoietic stem cells in bone marrow. A western blot assay was used to detect the changes of proteins involving TLR signaling pathway. Results: We found that HKMT increased cell viability and inhibited cell apoptosis after irradiation. HKMT induced NF-kB translocation and activated Erk1/2, p38 signaling pathway. HKMT also protected bone marrow and testis from destruction. Radiation-induced decreases of nucleated cells and CD34+ hemopoietic stem cells in bone marrow were also inhibited by HKMT treatment. We found that radiation caused increase of inflammatory cytokines was also suppressed by HKMT. Conclusion: Our data showed that HKMT exhibited radioprotective effects in vivo and in vitro through activating NF-kB and MAPK signaling pathway, suggesting a potential of HKMT as novel radioprotector.

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“…It has also been reported that use of H 2 -enriched saline in mice protects immune system function and spleen inflammation induced by radiation. Biomarkers of oxidative stress, inflammation, apoptosis and immune response capacity were all improved after hydrogen-enriched saline administration [295].…”

Section: Hydrogen In Inflammatory Diseasesmentioning

confidence: 93%

Clinical Effects of Hydrogen Administration: From Animal and Human Diseases to Exercise Medicine

Nicolson¹,

Ferreira²,

Settineri³

et al. 2016

IJCM

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Here we review the literature on the effects of molecular hydrogen (H2) on normal human subjects and patients with a variety of diagnoses, such as metabolic, rheumatic, cardiovascular and neurodegenerative and other diseases, infections and physical and radiation damage as well as effects on aging and exercise. Although the effects of H2 have been studied in multiple animal models of human disease, such studies will not be reviewed in depth here. H2 can be administered as a gas, in saline implants or infusions, as topical solutions or baths or by drinking H2-enriched water. This latter method is the easiest and least costly method of administration. There are no safety issues with hydrogen; it has been used for years in gas mixtures for deep diving and in numerous clinical trials without adverse events, and there are no warnings in the literature of its toxicity or longterm exposure effects. Molecular hydrogen has proven useful and convenient as a novel antioxidant and modifier of gene expression in many conditions where oxidative stress and changes in gene expression result in cellular damage.

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Protective effect of hydrogen‐rich saline against radiation‐induced immune dysfunction

Cited by 37 publications

References 23 publications

Pine polyphenols fromPinus koraiensisprevent injuries induced by gamma radiation in mice

Pine polyphenols fromPinus koraiensisprevent injuries induced by gamma radiation in mice

Radioprotective Effects of Heat-Killed Mycobacterium Tuberculosis in Cultured Cells and Radiosensitive Tissues

Clinical Effects of Hydrogen Administration: From Animal and Human Diseases to Exercise Medicine

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