Pine polyphenols fromPinus koraiensisprevent injuries induced by gamma radiation in mice

Abstract

Pine polyphenols (PPs) are bioactive dietary constituents that enhance health and help prevent diseases through antioxidants. Antioxidants reduce the level of oxidative damages caused by ionizing radiation (IR). The main purpose of this paper is to study the protective effect of PPs on peripheral blood, liver and spleen injuries in mice induced by IR. ICR (Institute of Cancer Research) male mice were administered orally with PPs (200 mg/kg b.wt.) once daily for 14 consecutive days prior to 7 Gy γ-radiations. PPs showed strong antioxidant activities. PPs significantly increased white blood cells, red blood cells and platelets counts. PPs also significantly reduced lipid peroxidation and increased the activities of superoxide dismutase, catalase and glutathione peroxidases, and the level of glutathione. PPs reduced the spleen morphologic injury. In addition, PPs inhibited mitochondria-dependent apoptosis pathways in splenocytes induced by IR. These results indicate that PPs are radioprotective promising reagents.

Protective Effects on 60Co-γ Radiation Damage of Pine Cone Polyphenols from Pinus koraiensis-Loaded Chitosan Microspheres In Vivo

“…Ionizing radiation induced the cells to produce excessive ROS, which leads to lipid peroxidation, breaks redox homeostasis within cells and living tissues, generates MDA, and decreases the activities of enzymatic and non-enzymatic antioxidants in vivo [ 26 , 27 , 28 ]. In the present study, as shown in Table 3 , administration of PP and PPM reduced the levels of MDA and restored the SOD activity of irradiated mice, which is consistent with a previous study that showed PP could reduce the redox imbalance and lipid peroxidation, and partly restore the redox balance of radiation-injured mice [ 21 ]. Many other studies also suggested that plant polyphenols had an anti-radiation and anti-oxidative stress effect by restoring the redox balance of the system [ 29 , 30 , 31 ].…”

“…Whole-body radiation can damage the mouse digestive system, immune system, and hematopoietic system, causing weight loss in mice [ 21 ]. PP and PPM reduced the decline in body weight, which indicated they may play a radioprotective effect.…”

“…PP from Pinus koraiensis were prepared according to the method of Li & Wang [ 21 ] and enriched using an X-5 macroporous resin chromatographic column (Shengda Co., Ltd., Harbin, China). The purity of the collected polyphenols was 40.2% according to the Folin–Ciocalteau method.…”

“…Plant polyphenols have been found to have significant radioprotective effects. In a previous study from this laboratory, polyphenols from Pinus koraiensis had a strong antioxidant activity with the ABTS·C radical [ 20 ] and could effectively prevent injuries induced by γ-radiation in mice [ 21 ]. In this study, in order to explore the effect of microspheres prepared by emulsion crosslinking technique on radiation protection, Ch microspheres prepared using an emulsion technique as a carrier for PP were compared to free PP in vivo using mice.…”

In Vitro bioassay-guided isolation of radioprotective fractions from extracts of Pinus koraiensis bark

“…In previous study, Li et al . reported that “Pine polyphenols of Pinus koraiensis inhibit damages insulted by radiation in mice,”[ 7 ] the mixture of pine polyphenols was purified from cone of P. koraiensis . Yun and Wang reported “Antioxidant activities of poly phenols from P. korainsis in vitro ,” the extract was prepared from P. koraiensis bark.…”