Protective effect of Aster tataricus extract on NLRP3‐mediated pyroptosis of bladder urothelial cells

Abstract

Aster tataricus L.f. is a traditional Eastern Asian herbal medicine used for the relief of uroschesis‐related illnesses and has been demonstrated clinically to exert satisfied effects. However, the mechanism of its therapeutic action remains unclear. The present study aimed to evaluate the protective mechanism of Aster tataricus extract (ATE) on CYP or LPS + ATP‐induced interstitial cystitis (IC), we successfully constructed the induced IC Sprague‐Dawley (SD) rat model and IC human urothelium cell (SV‐HUC‐1) model. The main compounds of ATE were determined by LC‐MS. After intervention, the changes on the bladder wall morphology and inflammation were observed in each group. SV‐HUC1 cell viability was measured by MTT and double stained with Hoechst 33342 and propidium iodide (PI). The expression levels of NLRP3, Pro‐caspase‐1, Caspsae‐1 p20, GSDMD, GSDMD‐N and Cleave‐IL‐1β in vivo and in vitro in different groups were detected by Western blotting. ATE significantly alleviated oedema and haemorrhage and reduced the inflammation index and histopathological score in SD rat bladder. The results of cell revealed that ATE could improve cell viability and decrease pyroptosis ratio. The expression of NLRP3 and other pyroptosis‐related protein was remarkably decreased by ATE both in vivo and in vitro. ATE may be used as an inhibitor of NLRP3 in treating IC. The discovery of NLRP3/Caspase‐1/GSDMD‐N as a new protective pathway provides a new direction for protecting cell against IC.

A Systematic Review of Therapeutic Approaches Used in Experimental Models of Interstitial Cystitis/Bladder Pain Syndrome

“…In IC/BPS, the use of several medicinal plants has been evaluated in experimental models. For example, Aster tataricus extract and its main active component Shionone were shown to reduce bladder inflammation in IC rats and decrease pyroptosis in the SV-HUC1 urothelial cell line, by inhibiting the NLRP3–GSDMD pathway [ 95 , 96 ], while intravesical treatment with Bletilla striata extract solution was shown to attenuate visceral hypersensitivity and bladder overactivity in zymosan-induced-IC in SD rats [ 97 ]. Treatment with Olea europaea or Juniperus procera leaf extracts in SD rats with stress (water deprivation)-induced IC reduced bladder mast cell infiltration and levels of stress hormones [ 98 ], and Houttuynia cordata extract inhibited mast cell proliferation and activation, decreased the levels of proinflammatory cytokines IL6, IL8, and TNFα, and reduced inter-contraction intervals in SD rats with CYP-induced IC [ 99 ].…”

Metabolomics and transcriptomics provide insights into the flavonoid biosynthesis pathway in the roots of developing Aster tataricus

“…A. tataricus has been used as a traditional medicinal herb in China for more than 2,000 years due to its medicinal properties, such as anti-oxidation, anti-inflammatory, and anti-cancer, for humans (Du et al 2017 ; Li et al 2021b ; Su et al 2019 ; Wang et al 2020 ). Li et al reported that the anti-lung cancer function of A. tataricus is closely related to flavonoids such as quercetin, kaempferol, isorhamnetin, and luteolin (Li et al 2021b ).…”

“…(2R,2′R)-7-O-methyl–2,3,2′′,3′′–tetrahydrorobustaflavone, a new flavonoid isolated from A. tataricus , can significantly inhibit A594 cancer cells proliferation (Chen et al 2022 ). As the largest group of secondary metabolites in plants, flavonoids not only contribute to diverse human health benefits, but also provide plants with a variety of biological functions, such as diseases treatment for humans and stress resistance for plants (Ng et al 2003 ; Treutter 2005 ; Wang et al 2020 ; Wen et al 2021 ). Flavonols may facilitate maize ( Zea mays ) seedling drought tolerance by scavenging H 2 O 2 and stomatal closure (Li et al 2021a ).…”

Radix Asteris: Traditional Usage, Phytochemistry and Pharmacology of An Important Traditional Chinese Medicine

“…In addition, an in vivo study [ 3 ] showed that the Fr-50 fraction (40, 80 mg/kg) of a 70% ethanolic extract from RA root significantly enhanced tracheal phenol red secretion, prolonged latency, reduced cough frequency, and suppressed mouse ear edema. Wang et al [ 42 ] found that extract of RA could reduce the edema and hemorrhage in the bladder of rats with interstitial cystitis, and extract of RA significantly reduced other pyrolysis of in vivo and in vitro death-related proteins. These results indicated that the different extracts of RA attenuated the inflammatory reaction by inhibiting various inflammatory mediators ( Figure 8 ).…”