Protective Effect ofBroussonetia papyriferaagainst Hydrogen Peroxide-Induced Oxidative Stress in SH-SY5Y Cells

Abstract

This study compared the antioxidant activity of various parts of Broussonetia papyrifera (BP), and further evaluated the protective effects of BP against hydrogen peroxide (H(2)O(2))-induced neuronal injury in human neuroblastoma SH-SY5Y cells. Among four BP parts, BP radix and leaf possessed the best scavenging activities for 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical, and H(2)O(2). These two BP parts also had higher phenolic and phenylpropanoid contents. Following exposure of cells to H(2)O(2), there was a marked decrease in cell survival, intracellular glutathione levels, and antioxidant enzymes, as well as an increase in intracellular oxidative stress. DNA fragmentation was also observed, but pretreatment of the cells with BP radix but not leaf prior to H(2)O(2) exposure blocked these H(2)O(2)-induced cellular events. The present findings indicate that BP radix exerts protective effects against H(2)O(2) toxicity via its free radical scavenging activity, which might be due to its total phenolic compounds.

Evaluation of anti-inflammatory effects of Broussonetia papyrifera stem bark

“…[18] About 42 compounds were isolated from the whole plants of B. papyrifera . [19] Phenolic compounds with antioxidant activity were isolated from the fruits of B. papyrifera against H 2 O 2 -induced neuronal injury in human neuroblastoma SH-SY5Y cells[2021] or against H 2 O 2 -induced impairment in PC12 cells. [22] Some broussonetones-like compounds isolated from the leaves of B. papyrifera have shown antioxidant and antityrosinase activities and they could be useful ingredients in the development of skin-protecting cosmetics.…”

Effect of Broussonetia papyrifera L. (paper mulberry) silage on dry matter intake, milk composition, antioxidant capacity and milk fatty acid profile in dairy cows

“…B. papyrifera L . had a protective effect against hydrogen peroxide-induced oxidative stress in human SH-SY5Y cells [ 3 ]. Prenylflavone derivatives from B. papyrifera L .…”

The Genus Broussonetia: A Review of its Phytochemistry and Pharmacology

“…The IC 50 values of B. kazinoki (stem) and B. papyrifera (leaves, bark) were lower than those of arbutin (a reference indicator, IC 50 = 76.00 µg/mL). In another study, Tsai et al () compared the antioxidant activities of the various parts (roots, leaves, stem, fruits) of B. papyrifera , and further evaluated the protective effects of B. papyrifera against hydrogen peroxide (H 2 O 2 )‐induced neuronal injury in human neuroblastoma SH‐SY5Y cells. Broussonetia papyrifera roots showed scavenging ability against free radicals, namely, 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH, IC 50 = 5.55 ± 0.34 mg of catechin/g), 2,2'‐azino‐bis‐3‐ethylbenz‐thiazoline‐6‐sulphonic acid (ABTS, IC 50 = 48.63 ± 4.99 µmol of trolox/g) radical and H 2 O 2 (IC 50 = 70.90 ± 2.90 µmol of trolox/g), among these parts.…”

“…Experimental studies have demonstrated extracts of the plants show antifungal, antihyperglycaemic, antityrosinase, antioxidant, antinociceptive and antiinflammatory activities (Kim et al , ; Iida et al , ; Cha et al , ; Lin et al , ; Tsai et al , ; Jin et al , ). Several bioactive substances including coumarins, polyphenols, alkaloids, glycosides, flavonoids, lignans and diterpenes were isolated from bioassay‐guided fractionation of the extracts of these plants (Takasugi et al , ; Gunatilaka et al , ; Ikuta et al , ; Kato et al , ; Dehmlow et al , ; Fang et al , ; Shibano et al , ; Son et al , ; Zhang et al , ; Mei et al , ).…”