Protective effect ofLepidium sativumseed extract against hydrogen peroxide-induced cytotoxicity and oxidative stress in human liver cells (HepG2)

Abstract

Objective: The present investigation was designed to study the protective effects of chloroform extract of Lepidium sativum seed (LSE) against oxidative stress and cytotoxicity induced by hydrogen peroxide (H 2 O 2 ) in human liver cells (HepG2). Materials and methods: Cytotoxicity of LSE and H 2 O 2 was identified by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), neutral red uptake (NRU) assays, and morphological changes in HepG2. The cells were pre-exposed to biologically safe concentrations (5-25 mg/ml) of LSE for 24 h, and then cytotoxic (0.25 mM) concentration of H 2 O 2 was added. After 24 h of the exposures, cell viability by MTT, NRU assays, and morphological changes in HepG2 were evaluated. Further, protective effects of LSE on reactive oxygen species (ROS) generation, mitochondrial membrane potential (MMP), lipid peroxidation (LPO), and reduced glutathione (GSH) levels induced by H 2 O 2 were studied.Results: Pre-exposure of LSE significantly attenuated the loss of cell viability up to 48% at 25 mg/ ml concentration against H 2 O 2 (LD 50 value ¼ 2.5 mM). Results also showed that LSE at 25 mg/ml concentration significantly inhibited the induction of ROS generation (45%) and LPO (56%), and increases the MMP (55%) and GSH levels (46%). Discussion and conclusion: The study suggests the cytoprotective effects of LSE against H 2 O 2 -induced toxicity in HepG2. The results also demonstrate the anti-oxidative nature of LSE.

Efficacy of Lepidium Sativum to act as an anti-diabetic agent

“…Al-Sheddi et al (2016) [50] observed that Lepidium sativum extract (LSE) at 25 µg/ml concentration significantly inhibited the induction of ROS generation (45%) and lipid peroxidation (56%), and increased the mitochondrial membrane potential (55%) and GSH levels (46%). The study suggests the cytoprotective and antioxidative effects of LSE against H 2 O 2 -induced toxicity in HepG2.…”

Hepatoprotective activity of Lepidium sativum seeds against D-galactosamine/lipopolysaccharide induced hepatotoxicity in animal model

“…Lepidium sativum L used as remedy for inflammatory diseases, such as diabetes, arthritis, traumatic injuries, and hepatitis in traditional medicine [19, 20]. Lepidium sativum L. extract is reported to have various in vitro biological effects including antioxidant, anti-inflammatory, antidiarrheal, antimicrobial, antispasmodic and hepatoprotective action against oxidative damage and therefore, have a great potential for use as herbal hepatoprotective or dietary supplements [21, 22]. Literature on phytochemical investigations of Lepidium sativum L. revealed the presence of benzyl isothiocyanate, flavonoids, tannins, triterpens, alkaloids, sterols, glucosinolates [20, 23] which were reported to have, antioxidant, anti-inflammatory, analgesic activities and hepatoprotective properties [24–26].…”

<i>Portulaca oleracea</i> Linn seed extract ameliorates hydrogen peroxide-induced cell death in human liver cells by inhibiting reactive oxygen species generation and oxidative stress

“…Cells were grown in 5 % CO 2 at 37 °C in high humidity atmosphere. Before the experiments, cell viability was assessed as described by Siddiqui et al [20]. HepG2 cells showing more than 98 % cell viability and at passage numbers 20 -22 were used in this study.…”