Protective effect of Morinda citrifolia fruits on β‐amyloid (25–35) induced cognitive dysfunction in mice: An experimental and biochemical study

Abstract

The neuroprotective effect of an ethyl acetate extract of Morinda citrifolia (Rubiaceae) Linn. fruits (EMC, ethyl acetate extract of Morinda citrifolia) at doses of 200 and 400 mg/kg, p.o. was studied on beta-amyloid (25-35) peptide induced cognitive dysfunction in mice. In the step-down inhibitory avoidance, EMC exhibited a significant increase in short-term memory and long-term memory (p < 0.05). A significant decrease (p < 0.01) in escape latency was noticed in the animals in the water maze. A significant increase (p < 0.01) in alteration of behavior was exhibited upon administration of EMC 200 and 400 mg/kg on the Y maze. Exploratory parameters such as line crossings, head dipping and rearing were increased significantly in EMC treated groups in a dose-dependent manner (p < 0.05 and p < 0.01). A significant reduction (p < 0.05) in acetyl cholinesterase activity was noticed in the EMC 200 and 400 mg/kg treated groups. The level of monoamine oxidase-A was decreased by the administration of EMC 200 and 400 mg/kg (p < 0.05 and p < 0.01, respectively). EMC at a dose of 400 mg/kg exhibited a significant increase (p < 0.01) in the levels of serotonin and dopamine. Antioxidant enzymes such as superoxide dismutase, glutathione reductase, glutathione peroxidase and ascorbic acid were decreased significantly in the b-amyloid peptide injected group, whose levels were restored significantly (p < 0.01) by the administration of EMC (400 mg/kg).

Neuroprotective effect of Alpinia galanga (L.) fractions on Aβ(25–35) induced amnesia in mice

“…A␤ (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) is potential neurotoxic peptide for primary neuronal cortical cells which produces neurofibrillary tangles (Glenner and Wong, 1984;Masters et al, 1985) and the toxicity induced by A␤ (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) in rodents resembles to AD which is suitable for the evaluation of Alzheimer's type of dementia. Previous studies suggested that accumulation of A␤ induces the reduction of habituation memory in open field exploration (Muralidharan et al, 2010) and single administration of A␤ (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) in mouse through i.c.v exhibited marked amnesic effects in short term memory as well as in long term memory, in addition impaired spatial learning in water maze (Maurice et al, 1996). Water maze evaluation is an aversive method for determining cognitive function of hippocampal memory.…”

Protective effect of fruits of Morinda citrifolia L. on scopolamine induced memory impairment in mice: A behavioral, biochemical and cerebral blood flow study

“…Moreover, Noni fruit juice has been reported to prevent neuronal damage induced by focal ischemia (Harada et al, 2009). Recently, Muralidharan et al (2010) reported that ethyl acetate extract of Noni prevented memory impairment and oxidative stress induced by amyloid beta peptide in mice.…”

In vitro & ex vivo Acetylcholinesterase Inhibitory Activity of Morinda citrifolia Linn (Noni) Fruit Extract

“…These activities were carried out to the extent of in-vitro, in-vivo & clinical trial stages. 8 The neuroprotective effect of an ethyl acetate fruit extract of Morinda citrifolia (Rubiaceae) Linn at doses of 200 and 400 mg/kg, po was contemplated by Muralidharan et al 9 Past study by Pachauri et al was intended to examine impact of Noni on memory, cerebral blood flow(CBF), oxidative stress and AChE action in scopolamine incited amnesia mice model. 10 Harada et al inspected the impact of the juice of Morinda citrifolia (Noni juice) on the brain damage brought about by ischemic stress in mice.…”