Protective effect of Khamira Abresham Uood Mastagiwala against free radical induced damage in focal cerebral ischemia

Yousuf, Seema; Salim, Sofiyan; Ahmad, Abdullah Shafique; Ahmed, Abdul‐Rahman; Ansari, Mubeen A.; Islam, Fakhrul

doi:10.1016/j.jep.2004.12.035

Cited by 36 publications

(23 citation statements)

References 18 publications

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“…Behavioral examination showed a significant improvement in motor coordination and spontaneous motor activity. Earlier studies have shown that treatment with antioxidant improved a motor impairment [31,32]. The motor function was found to be disturbed in both the general motor and rota rod tasks.…”

Section: Discussionmentioning

confidence: 92%

Synergistic Effect of Selenium and Melatonin on Neuroprotection in Cerebral Ischemia in Rats

Ahmad

Khan

Ishrat

et al. 2010

Biol Trace Elem Res

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The synergistic scavenger effects of selenium and melatonin collectively we called Se-Mel was studied on the prevention of neuronal injury induced by ischemia/reperfusion. Male Wistar rats were treated with sodium selenite (0.1 mg/kg, i.p.) and melatonin (10 mg/kg, i.p.) 30 min before the middle carotid artery occlusion (MCAO) and immediately after MCAO to male Wistar rats and was continued for 3 days once daily at the interval of 24 h. Behavioral activity (spontaneous motor activity and motor deficit) was improved in Se-Mel-treated rats as compared to MCAO group rats. The level of glutathione and the activity of antioxidant enzymes was depleted significantly while the content of thiobarbituric acid reactive substances, protein carbonyl, and nitric oxide radical (NO(·)) was increased significantly in MCAO group. Systemic administration of Se-Mel ameliorated oxidative stress and improves ischemia/reperfusion-induced focal cerebral ischemia. Se-Mel also inhibited inducible nitric oxide synthase expression in Se-Mel+MCAO group as compared to MCAO group rats. Thus, Se-Mel has shown an excellent neuroprotective effect against ischemia/reperfusion injury through an anti-ischemic pathway. In conclusion, we demonstrated that the pretreatment with Se-Mel at the onset of reperfusion, reduced post-ischemic damage, and improved neurological outcome following transient focal cerebral ischemia in male Wistar rat.

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Section: Discussionmentioning

confidence: 92%

Synergistic Effect of Selenium and Melatonin on Neuroprotection in Cerebral Ischemia in Rats

Ahmad

Khan

Ishrat

et al. 2010

Biol Trace Elem Res

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“…Although the reason of this is not yet known, it is believed that it is due to the prevention of the oxidative stress, as has been noticed for other metal oxide particles such as CeO 2 , 39,40 Y 2 O 3 , 41 and other Bhasma powders. 38,42 Furthermore, establishing these spectral signatures of particulate matter (Bhasma) interaction with the cells is expected to lead to reliable monitoring of cellular response by Raman spectroscopy.…”

Section: Resultsmentioning

confidence: 99%

“…MTT assay shows that Bhasma treatment increased the mitochondrial activity of A549 cells, perhaps due to ROS scavenging in the Bhasma-treated cells. 38 Raman Spectra, Peaks, and Molecular Information…”

Section: Growth and Morphologymentioning

confidence: 99%

Cell (A549)–Particle (Jasada Bhasma) interactions using Raman spectroscopy

et al. 2008

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Current methods for the evaluation of cell interactions with particles are nonspecific, slow, and invasive to the cells. Raman spectroscopy is a noninvasive technique, and is used in the present study to investigate particle-cell interactions. The main focus of the present study is to employ Raman spectroscopy for investigating the interaction of human lung adenocarcinoma cell line (A549) with the particulate system Jasada Bhasma, a traditional Indian medicine. Jasada Bhasma is a unique preparation of zinc and is traditionally used for the treatment of various diseases like diabetes, age-related eye diseases, and as a health promotional tonic. The Raman spectral analysis is executed by identifying the difference in intracellular DNA/RNA, and proteins and lipids concentration between particles--treated and untreated cells. Comparison between Bhasma-treated and -untreated cells indicates that vibrational peaks corresponding to the DNA/RNA molecule show a significant increase in cells treated with the Jasada Bhasma. Apart from the DNA molecule, several other vibrational peaks related to the protein molecules also show a significant increase in A549 cells after treatment with Bhasma. These results indicate that Bhasma treatment of A549 possibly delays DNA degradation and enables retention of higher amount of protein molecules in the cells.

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“…The brain is quite susceptible to oxidative stress due to its environment with non-heme iron, which is involved catalytically in the production of free radicals (26). The close relationship between oxidative stress and cerebral ischemia has generated considerable interest in the development of antioxidant therapies to combat ischemia-induced damage (27,28).…”

Section: Discussionmentioning

confidence: 99%

Korean red ginseng protects against neuronal damage induced by transient focal ischemia in rats

Ban

Kang

Lee

et al. 2012

Experimental and Therapeutic Medicine

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Abstract. In the present study, we investigated the neuroprotective effect of Korean red ginseng (KRG) following focal brain ischemia/reperfusion injury, in relation to its antioxidant activities. The middle cerebral artery occlusion/reperfusion (MCAO/R) model in rats was employed. The KRG extract (100 mg/kg, perorally) was administered once daily for 7 days following MCAO/R. The elevated levels of lipid peroxidation in the MCAO/R group were attenuated significantly in the KRG-administered group. The significantly depleted activity of the antioxidant enzymes glutathione peroxidase, superoxide dismutase and catalase was prevented in the KRG-administered group. In the neurobehavioral evaluation expressed as the modified neurological severity score and corner-turn test, the daily intake of KRG showed consistent and significant improvement in the neurological deficits for 7 days following MCAO/R injury. These results indicate that KRG has a neuroprotective effect against ischemia/reperfusion brain injury by reducing the level of lipid peroxidation and increasing the endogenous antioxidant enzymatic activity.

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Protective effect of Khamira Abresham Uood Mastagiwala against free radical induced damage in focal cerebral ischemia

Cited by 36 publications

References 18 publications

Synergistic Effect of Selenium and Melatonin on Neuroprotection in Cerebral Ischemia in Rats

Synergistic Effect of Selenium and Melatonin on Neuroprotection in Cerebral Ischemia in Rats

Cell (A549)–Particle (Jasada Bhasma) interactions using Raman spectroscopy

Korean red ginseng protects against neuronal damage induced by transient focal ischemia in rats

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