Protective effect of L-arginine on gentamicin-induced nephrotoxicity in rats

Abstract

Introduction:L-arginine has a protective effect on gentamicin-induced renal failure and it may decrease the tubular reabsorption of another cationic substance, gentamicin due to its cationic structure. The aim of this study is to compare the possible protective effects of L-arginine and its inactive isomer D-arginine on gentamicin-induced nephrotoxicity in rats.Materials and Methods:Wistar albino rats were housed in metabolic cages and assigned to six groups as: control group, gentamicin (100 mg/kg), gentamicin + L-arginine (2 g/l), gentamicin + D-arginine (2 g/l), gentamicin + L-arginine + Nv-nitro-L-arginine methyl ester (L-NAME) (100 mg/l) and gentamicin + D-arginine + L-NAME. Gentamicin was administered by subcutaneous injections and the other drugs were added in drinking water for seven consecutive days. The animals were killed by decapitation and intracardiac blood and urine samples were obtained on the seventh day. Blood urea nitrogen, serum creatinine, sodium, potassium, urine gamma glutamyl transferase, creatinine, sodium, potassium and gentamicin levels were measured using High Performance Liquid Chromatography (HPLC) technique.Results:Gentamicin treated group had significant increase in blood urea nitrogen, serum creatinine, fractional Na excretion and urine gamma glutamyl transferase levels, and significant decrease in creatinine clearance compared to the control group. L-arginine and D-arginine reversed these findings. L-NAME abolished the nephroprotective effect of L-arginine. The urinary levels of gentamicin were significantly increased in rats treated with L-arginine or D-arginine compared to those treated with gentamicin. L-arginine and D-arginine reversed the advanced degenerative changes due to gentamicin administration in histopathological examination.Conclusion:Our study revealed the protective effect of L-arginine on gentamicin-induced nephrotoxicity, the contribution of the cationic feature of L-arginine, and the major role of NO in this protective effect.

Nephroprotective Effects of L-Arginine against Chemotherapy Induced Acute Kidney Injury in Wistar Rats

“…These findings are similar to the results reported by Wang et al and Adeneye and Benebo. 19,20 However, blood urea and creatinine levels in the rats receiving adjunct therapy of L-arginine were far less than those who received Cisplatin alone which is consistent with the findings of Başhan et al 21 Saleh et al also reported a significant rise in blood urea and creatinine levels in experimental animals which received cisplatin but the experimental animals receiving concomitant arginine therapy showed near normal levels, which is consistent with the current study. 22 In this experiment, there was an increase in the CRP levels and decrease in the serum GPX levels of cisplatin-treated rats denoting inflammation and oxidative stress, respectively.…”

Pimpinella anisum L. ethanolic extract ameliorates the gentamicin‐ induced nephrotoxicity in rats

“…The study included 40 male Sprague–Dawley rats weighing 200 ± 50 g, randomly divided into four groups ( n = 10). All rats were housed in an environment controlled at 23 ± 2°C, with free access to food and water and a light/dark cycle of 12:12 h. All tests and procedures were conducted in compliance with the guidelines for the care and handling of animals prepared by the Iranian Ministry of Health (Ethics board approval number: Arakmu.REC.1392.151.6) and internationally accepted principles for laboratory animal use and care as found in the European Community Guidelines (EEC Directive of 1986; 86/609/EEC) …”

Phytochemical, pharmacological evaluation of Morinda pubescens J.E.Sm. bark extract for nephroprotective activity

“…An association between nephrotoxicity and oxidative stress has been confirmed in many experimental models. [ 30 31 32 ]…”