Protective Effect of Lactobacillus rhamnosus GG on TiO2 Nanoparticles-Induced Oxidative Stress Damage in the Liver of Young Rats

Abstract

The potential toxicity of titanium dioxide nanoparticles (TiO2 NPs) to mammals has become a widespread concern. Young individuals exposed to TiO2 NPs have a higher risk than adults. In this study, the protective effects of Lactobacillus rhamnosus GG (LGG) on liver toxicity in young rats induced by TiO2 NPs were explored. Results show that the four-week-old rats that underwent LGG after the oral intake of TiO2 NPs could prevent weight loss, reduce hematological indicators (WBC and NEUT) and serum biochemical indicators (AST, ALT, AST/ALT, and ALP). Moreover, it alleviated the pathological damage of the liver (as indicated by the disordered hepatocytes, more eosinophilic, ballooning degeneration, and accompany with blood cells), but it did not reduce the Ti contents in the liver. In addition, RT-qPCR results indicated that LGG restored the expression of anti-oxidative stress-related genes, such as SOD1, SOD2, CAT, HO-1, GSH, GCLC, and GCLM in the liver. In summary, the hepatotoxicity of TiO2 NPs in young rats is closely related to oxidative stress, and the antioxidant effect of LGG might protect the harmful effects caused by TiO2 NPs.

Antioxidants and mitochondrial/lysosomal protective agents reverse toxicity induced by titanium dioxide nanoparticles on human lymphocytes

“…All these oxidative stress and cytotoxicity markers were prevented by preincubation with BHT as an antioxidant agent for human lymphocytes. Consistent with our results, other antioxidant agents attenuated TiO 2 NPs-ROS formation and oxidative stress (Afshari-Kaveh et al, 2021; Nie et al, 2021). It has been reported that accumulation of ROS results in mitochondrial permeability transition (mPT) and mPT pore opening (Kowaltowski et al, 2001).…”

Combined exposure of nano‐titanium dioxide and polystyrene nanoplastics exacerbate oxidative stress‐induced liver injury in mice by regulating the Keap‐1/Nrf2/ARE pathway

“…It is worth noting that TiO 2 is allowed to be added to animal feed additives in China, so in the process of feeding mice, TiO 2 was ingested and accumulated in the body, resulting in a certain amount of Ti in mouse liver in Control group. Ti contents in the liver of mice exposed to TiO 2 NPs was only twice that of Control group, which is regarded as a normal phenomenon, and in our preliminary laboratory studies have also indicated similar experimental results 51 …”

Lactobacillus rhamnosus GG Ameliorated Long-Term Exposure to TiO2 Nanoparticles Induced Microbiota-Mediated Liver and Colon Inflammation and Fructose-Caused Metabolic Abnormality in Metabolism Syndrome Mice

“…LGG is a natural and commercial probiotic with excellent anti-inflammation and antioxidation functions . Numerous researchers used LGG to treat inflammation-induced organs or tissues damage. , In our previous work, we explored the protective effect of LGG on TiO 2 NPs-induced liver and intestine inflammation injury in juvenile rats. , Therefore, we supplemented MetS mice with LGG to protect against fructose and TiO 2 NPs-caused liver and colon damage. After the treatment with LGG, the TiO 2 NPs-induced excessive inflammation factors were reduced, and the increase in gut permeability was effectively prevented, which can explain the decreased feces and serum LPS level (Figure ).…”