Protective Effect of Montmorillonite on Plasmid DNA in Oral Gene Delivery into Small Intestine

Kawase, Masaya; Hayashi, Yuka; Kinoshita, Fumie; Yamato, Eiji; Miyazaki, Jun‐ichi; Yamakawa, Junji; Ishida, Tomoyuki; Tamura, Manabu; Yagi, Kiyohito

doi:10.1248/bpb.27.2049

Cited by 42 publications

(31 citation statements)

References 12 publications

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“…299 In vivo experiments carried out with a montmorillonite-plasmid DNA material confirmed the suitability of this type of silicate-DNA biohybrids for application as a non-viral vector for gene-delivery. 300 Oral administration of the montmorillonite-plasmid DNA material in mice showed the successful transfection of the plasmid into the cells of the small intestine. Given that transfection was not observed for the naked plasmid, this result suggests the protective effect of inorganic support on the plasmid from the acidic environment in the stomach and DNA-degrading enzymes in the intestine.…”

Section: Nucleic Acids Assembling To Silica and Silicatesmentioning

confidence: 99%

Hybrid and biohybrid silicate based materials: molecular vs. block-assembling bottom–up processes

2011

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Section: Nucleic Acids Assembling To Silica and Silicatesmentioning

confidence: 99%

Hybrid and biohybrid silicate based materials: molecular vs. block-assembling bottom–up processes

2011

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“…[54,55] HAp is not the only candidate for DNA protection: for example, silicates share some structural characteristics with apatite which would be compatible with DNA interactions. [56,57] Montmorillonite, illite, and kaolinite represent clay minerals that are able to protect DNA from nucleases during natural bacterial genetic transformation [58,59] and they are also useful for gene delivery as silica nanoparticles, but their slow degradation rate decreases efficiency [60,61] compared to HAp.…”

Section: Cell Deathmentioning

confidence: 99%

Surviving Mass Extinctions through Biomineralized DNA

Turón

Puiggalı́

Bertrán

et al. 2015

Chemistry A European J

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Even in the worst of conditions, such as those which occurred during mass extinction events, life on Earth never totally stopped. Aggressive chemical and physical attacks able to sterilize or poison living organisms occurred repeatedly. Surprisingly, DNA was not degraded, denatured or modified to the point of losing the capability of transferring the genetic information to the next generations. After the events of mass extinction life was able to survive and thrive. DNA was passed on despite being an extremely fragile biomolecule. The potential implications of hydroxyapatite protection of DNA are discussed including how DNA acts as a template for HAp formation, how cell death can trigger biomineralizatoin, and how DNA can be successfully released from HAp when the conditions are favourable for life.

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“…However, there is great concern about safety because these procedures require physical force against organs; consequently, the continuous and repetitive administration of pDNA is limited. However, although pDNA complexes with chitosan [13], N-acetylated chitosan [14] and montmorillonite [15] were studied for oral gene delivery, the oral route has many hampers reducing transfection efficiency such as a low pH, high concentrations of digestive fluid, and rapid turnover of epithelial cells, etc.…”

Section: Introductionmentioning

confidence: 99%

Improved stomach selectivity of gene expression following microinstillation of plasmid DNA onto the gastric serosal surface in mice

Nishi

Fumoto

Ishii

et al. 2008

European Journal of Pharmaceutics and Biopharmaceutics

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Stomach-selective gene transfer is a promising approach as a therapeutic strategy for refractory gastric diseases. In this study, we improved the stomach selectivity of gene expression following microinstillation of naked plasmid DNA (pDNA) onto the gastric serosal surface in mice. pDNA encoding firefly luciferase was used as a reporter gene. It was confirmed that the gene expression level in the stomach 6 h after gastric serosal surface microinstillation of pDNA was significantly higher than after intragastric, intraperitoneal and intravenous administration. Regarding selectivity of gene expression, the gene expression level in the stomach after gastric serosal surface microinstillation of 1 µg/1 µL (dose/volume) pDNA was 5.7 times higher than that in the spleen. In our previous study (30 µg/30 µL), the expression level in the stomach was 2.7 times higher than that in the spleen; therefore, the selectivity was 2.1 times higher in this study. When we investigated gene expression at various pDNA solution concentrations, the ratio of the gene expression level in the stomach to that in the spleen was the highest as 1 µg/1 µL of pDNA, which was considered the optimal concentration. Information in this study is useful for further development of target organ-selective gene delivery systems.

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Protective Effect of Montmorillonite on Plasmid DNA in Oral Gene Delivery into Small Intestine

Cited by 42 publications

References 12 publications

Hybrid and biohybrid silicate based materials: molecular vs. block-assembling bottom–up processes

Hybrid and biohybrid silicate based materials: molecular vs. block-assembling bottom–up processes

Surviving Mass Extinctions through Biomineralized DNA

Improved stomach selectivity of gene expression following microinstillation of plasmid DNA onto the gastric serosal surface in mice

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