Protective Effect of Polysaccharide Fractions from Radix A. Sinensis against tert-Butylhydroperoxide Induced Oxidative Injury in Murine Peritoneal Macrophages

Yang, Xingbin; Zhao, Yan; Lv, You; Yang, Ying; Ruan, Yun

doi:10.5483/bmbrep.2007.40.6.928

Cited by 14 publications

(13 citation statements)

References 24 publications

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“…At three days post-exposure, the recover of MDA concentration might be due to the diminution of GST specific activity. Our data are different compared to previous findings which highlighted that the water-soluble polysaccharides from radix A. sinensis (Yang et al, 2007) and H. cumingii (Hu et al, 2010) lowered the lipid peroxidation in murine peritoneal macrophages and in rat brain.…”

Section: Antioxidant Enzymescontrasting

confidence: 56%

Water Soluble <i>Pleurotus ostreatus</i> Polysaccharide Down-Regulates the Expression of MMP-2 and MMP-9 in Caco-2 Cells

Cojocaru

Radu

Bodea

et al. 2013

Not Bot Hort Agrobot Cluj

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Many polysaccharides and polysaccharide-protein complexes isolated from mushrooms have immunomodulatory and anti-cancer effects. Our aim was to study the regulatory mechanisms of Caco-2 cell response to water soluble P. ostreatus polysaccharide extract up to 72 hours. Specific enzymatic activities were assessed by kinetic measurements. The reduced glutathione content and the lipid peroxidation level were also analyzed. Protein expression of several heat shock proteins, Bcl-2 and metalloproteinases 2 and 9 were revealed by Western blot. Gelatin zymography assay was used to evaluate the MMP-2 and MMP-9 activities. Until the third day of exposure the total SOD activity decreased continuously by 30%, whereas GST and GR ones diminished by 17% respectively 30.5% compared to control. No significant changes were observed in CAT and G6PDH specific activities as well as in GSH and MDA concentration. After the third day of exposure a significant up-regulation of Hsp60 and Hsp90 expression and a down-regulation of Hsp70 one were registered. Bcl-2 protein levels were down-regulated by 50% in the first day of treatment but increased after 3 days. MMP-2 and 9 secretion in the culture medium was significantly reduced suggesting a diminished ability of invasion of colon cancer cells. Our data revealed that in vitro treatment with P. ostreatus aqueous polysaccharide extract does not induce apoptosis in Caco-2 cell line but it could inhibit the invasion of colon cancer cells through the basement membrane.

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Section: Antioxidant Enzymescontrasting

confidence: 56%

Water Soluble <i>Pleurotus ostreatus</i> Polysaccharide Down-Regulates the Expression of MMP-2 and MMP-9 in Caco-2 Cells

Cojocaru

Radu

Bodea

et al. 2013

Not Bot Hort Agrobot Cluj

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“…Our approach was to use LPS-stimulated murine peritoneal macrophages as a vitro model to evaluate the effects of BP5 on oxidative stress in living cells. Numerous experimental studies show that macrophages are sensitive to LPS derived from gramnegative bacteria and thus provide an inducible form of macrophage that undergoes oxidative stress and secretes lysozyme upon activation [14][15][16]. This allows the evaluation of BP5 on intercellular redox status, gene profiles, and functional characteristics of macrophages in both their activated and non-activated forms.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the redox balance is an important determinant of macrophage activity. Previous reports have established that LPS can induce oxidative stress in murine peritoneal macrophages [14][15][16]. We used a living cell system of LPS-stimulated murine peritoneal macrophages as an oxidative stress model to find out whether BP5 can modulate macrophage activity.…”

Section: Introductionmentioning

confidence: 99%

The Suppressive Effects of Bursopentine (BP5) on Oxidative Stress and NF-ĸB Activation in Lipopolysaccharide-activated Murine Peritoneal Macrophages

Mao-yun

Geng

et al. 2012

Cell Physiol Biochem

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Background/Aim: Bursopentine (BP5) is a novel thiol-containing pentapeptide isolated from chicken bursa of Fabricius, and is reported to exert immunomodulatory effects on B and T lymphocytes. It has been found that some thiol compounds, such as glutathione (GSH) and N-acetylcysteine (NAC) protect living cells from oxidative stress. This led us to investigate whether BP5 had any ability to protect macrophages from oxidative stress as well as any mechanism that might underlie this process. Methods: Murine peritoneal macrophages activated by lipopolysaccharide (LPS) (2 µg/ml) were treated with single bouts (0, 25, 50, and 100 µM) of BP5. Results: BP5 potently suppressed the markers for oxidative stress, including nitric oxide (NO), reactive oxygen species (ROS), lipid peroxidation, and protein oxidation. It also decreased the expression and activity of inducible nitric oxide synthase (iNOS) and promoted a protective antioxidant state by elevating GSH content and by activating the expression and activity of certain key antioxidant and redox enzymes, including glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD) and catalase (CAT). This suppressive effect on oxidative stress was accompanied by down-regulated expression and activity of nuclear factor kappa B (NF-ĸB). Conclusion: These findings demonstrate that BP5 can protect LPS-activated murine peritoneal macrophages from oxidative stress. BP5 may have applications as an anti-oxidative stress reagent.

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“…•− through Fenton reactions, contributing to a series of pathological events characterized by reduction of the activities of antioxidant enzymes (Túnez et al, 2006;Valentão et al, 2004), lipid peroxidation (Jung et al, 2007;Palmer et al, 1988;Yang et al, 2007), induction of mitochondrial permeability transition (MPT) (Akao et al, 2003;Vercesi et al, 1997), Ca 2+ overload (Chu et al, 2008;Gen et al, 2001;Schomig et al, 1984), necrosis and apoptosis (Aikawa et al, 2002;Kuramochi et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…The NO production was determined indirectly by assaying the culture supernatant for accumulated nitrite, the stable end product of NO reacted with molecular oxygen, as described by Yang et al (2007). Briefl y, H9C2 cells were inoculated at 2 × 10 6 cells/well in a 96-well microplate for 24 h. After the treatments, 100 μL of isolated supernatants was allowed to react with Griess reagent (1% sulfanilamide, 0.1% N-1-naphthylethylenediamine dihydrochloride and 2.5% phosphoric acid) at room temperature for 10 min.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of steroids from Allium chinense against H₂O₂‐induced oxidative stress in rat cardiac H9C2 cells

Ren

Qiao

Yang

et al. 2009

Phytotherapy Research

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Allium chinense, a traditional herbal medicine, has been used for the treatment of cardiovascular diseases for hundreds of years. In this study, A. chinense steroids (ACSs) including three steroidal glycosides and their parent aglycones were isolated from the bulbs of A. chinense. For the first time, their cardioprotective effects were evaluated in cultured rat cardiac H9C2 cells by pretreatment with ACSs for 24 h before exposure to 0.2 mm H(2)O(2). The results showed the cell viability decreased markedly when H9C2 cells were incubated with 0.2 mm H(2)O(2) alone for 2 h, while the cell lipid peroxidation (estimated by the excessive production of nitric oxide and malondialdehyde) and intracellular free calcium concentration ([Ca(2+)](i)) increased significantly. The addition of 20 microm (below the toxic concentration) of ACSs notably attenuated the cellular injury induced by H(2)O(2). The effects of ACSs in our experiments were similar to those of nimodipine, a clinically applied calcium channel blocker. Preliminary analysis of the structure-activity relationship indicated that ACSs with a spirostane-type skeleton exhibited stronger protection than that with a furostane-type skeleton, and glycosylation of the steroids could substantially lower the protective activities. The above results suggested the protective effects of steroids originated from A. chinense on the oxidative injury of H9C2 cells and ACSs may have potential for preventing cardiac injuries induced by oxidative stress.

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Protective Effect of Polysaccharide Fractions from Radix A. Sinensis against tert-Butylhydroperoxide Induced Oxidative Injury in Murine Peritoneal Macrophages

Cited by 14 publications

References 24 publications

Water Soluble <i>Pleurotus ostreatus</i> Polysaccharide Down-Regulates the Expression of MMP-2 and MMP-9 in Caco-2 Cells

Water Soluble <i>Pleurotus ostreatus</i> Polysaccharide Down-Regulates the Expression of MMP-2 and MMP-9 in Caco-2 Cells

The Suppressive Effects of Bursopentine (BP5) on Oxidative Stress and NF-ĸB Activation in Lipopolysaccharide-activated Murine Peritoneal Macrophages

Protective effects of steroids from Allium chinense against H₂O₂‐induced oxidative stress in rat cardiac H9C2 cells

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Protective Effect of Polysaccharide Fractions from Radix A. Sinensis against tert-Butylhydroperoxide Induced Oxidative Injury in Murine Peritoneal Macrophages

Cited by 14 publications

References 24 publications

Water Soluble <i>Pleurotus ostreatus</i> Polysaccharide Down-Regulates the Expression of MMP-2 and MMP-9 in Caco-2 Cells

Water Soluble <i>Pleurotus ostreatus</i> Polysaccharide Down-Regulates the Expression of MMP-2 and MMP-9 in Caco-2 Cells

The Suppressive Effects of Bursopentine (BP5) on Oxidative Stress and NF-ĸB Activation in Lipopolysaccharide-activated Murine Peritoneal Macrophages

Protective effects of steroids from Allium chinense against H2O2‐induced oxidative stress in rat cardiac H9C2 cells

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Protective effects of steroids from Allium chinense against H₂O₂‐induced oxidative stress in rat cardiac H9C2 cells