Protective effect of polysaccharides of sea cucumber Acaudina leucoprocta on hydrogen peroxide-induced oxidative injury in RAW264.7 cells

Zhou, Tianyi; Xiang, Xingwei; Du, Ming; Zhang, Lei-Fang; Cheng, Nai-Xue; Liu, Xuanlin; Zheng, Bin; Wen, Zheng-Shun

doi:10.1016/j.ijbiomac.2019.08.092

Cited by 28 publications

(16 citation statements)

References 34 publications

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“…This allows successful nuclear translocation and cytoprotective gene transcription. A similar effect of antioxidant polysaccharides was reported by Zhou et al (2019) [37].…”

Section: Discussionsupporting

confidence: 86%

Antioxidant Potential of Sulfated Polysaccharides from Padina boryana; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model

et al. 2020

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Elevated levels of reactive oxygen species (ROS) damage the internal cell components. Padina boryana, a brown alga from the Maldives, was subjected to polysaccharide extraction. The Celluclast enzyme assisted extract (PBE) and ethanol precipitation (PBP) of P. boryana were assessed against hydrogen peroxide (H2O2) induced cell damage and zebra fish models. PBP which contains the majority of sulfated polysaccharides based on fucoidan, showed outstanding extracellular ROS scavenging potential against H2O2. PBP significantly declined the intracellular ROS levels, and exhibited protection against apoptosis. The study revealed PBPs’ ability to activate the Nrf2/Keap1 signaling pathway, consequently initiating downstream elements such that catalase (CAT) and superoxide dismutase (SOD). Further, ROS levels, lipid peroxidation values in zebrafish studies were declined with the pre-treatment of PBP. Collectively, the results obtained in the study suggest the polysaccharides from P. boryana might be a potent source of water soluble natural antioxidants that could be sustainably utilized in the industrial applications.

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“…This allows successful nuclear translocation and cytoprotective gene transcription. A similar effect of antioxidant polysaccharides was reported by Zhou et al (2019) [37].…”

Section: Discussionsupporting

confidence: 86%

Antioxidant Potential of Sulfated Polysaccharides from Padina boryana; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model

et al. 2020

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“…Polysaccharides containing l -fucose repeat units have excellent biological properties such as antioxidation and antitumor. For instance, polysaccharides from sea cucumber Acaudina leucoprocta have protective effects against hydrogen peroxide-induced oxidative injury in RAW264.7 . Since CA also contains l -fucose repeat units, it might have similar application potential.…”

Section: Resultsmentioning

confidence: 99%

“…All rheological characterizations of CA were measured on a Physica-MCR 302 rheometer (Anton Paar, Graz, Austria) equipped with a double-slit cup (outer diameter 26.7 mm, bore diameter 24.7 mm, and length 40 mm). To test the effect of CA concentrations on the viscosity, different concentrations of purified CA aqueous solutions (5,10,20,30,40,60, and 80 g/L) were prepared and tested at 25 °C and shear rates from 1 to 1000/s. Similarly, the viscosities of purified CA aqueous solutions with different concentrations were investigated at the shear rate of 100/s and temperatures from 25 to 90 °C at a heating rate of 6 °C/ min.…”

Section: Steepest Ascent Methodmentioning

confidence: 99%

Colanic Acid: Biosynthetic Overproduction by Engineering Escherichia coli and Physical Property Characterization

Qiao

Zhan

Tan

et al. 2021

J. Agric. Food Chem.

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Colanic acid has promising applications in food, cosmetic, and healthcare fields. In this study, a recombinant WQM003/pRAU was constructed by deleting genes lon and hns and overexpressing genes rcsA and galU in E. coli MG1655Δ(L-Q). After systematic optimization of fermentation conditions, colanic acid yield in WQM003/pRAU reached 19.79 g/L, the highest yield reported so far. The colanic acid produced by WQM003/pRAU was purified and its structure and physical properties were determined. This colanic acid shows a triple-helical structure and is stable up to 102 °C, and its melting temperature is 253.9 °C. This colanic acid shows a sphere-like chain conformation in aqueous solution. The viscosity of this colanic acid solution is related to concentration, shear rate, salt, temperature, and pH. At high concentrations, this colanic acid shows both viscous and elastic behaviors. These results suggest that the colanic acid produced by WQM003/pRAU has broad application prospects.

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“…The cell viability was measured using the CCK-8 assay according to the manufacturer's instructions and the hydrogen peroxide induced RAW264.7 cells oxidative stress model was established following the method described by Zhou et al with some modifications ( 34 ). RAW264.7 cells were seeded in 96-well plates (Eppendorf, Germany) at a density of 6 × 10 4 /ml in culture medium for 4 h. Subsequently, the cells were incubated with DMEM containing different concentrations of L. aureum extracts (dissolved in DMSO) or H 2 O 2 for 24 h. The viability of cells stimulated with L. aureum under oxidative stress were determined as follows: cells were plated in 96-well plates (Eppendorf, Germany) with a density of 1 × 10 5 in culture medium for 4 h, and the cells were incubated with DMEM containing different concentrations of L. aureum extracts (0, 2, 5, 10 μg/ml) for 20 h. The positive control group and the L. aureum –treated groups were then exposed to H 2 O 2 (400 μM) for 4 h. CCK-8 solution (10 μl) was added to each well and incubated in an atmosphere of 5% CO 2 at 37°C for 4 h. The absorption values were measured at 450 nm by using a spectrophotometer (Epoch Microplate Spectrophotometer; BioTek Instruments).…”

Section: Methodsmentioning

confidence: 99%

Antioxidant Effects and Potential Molecular Mechanism of Action of Limonium aureum Extract Based on Systematic Network Pharmacology

Yang

Cheng

et al. 2022

Front. Vet. Sci.

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Oxidative stress is the redox imbalance state of organisms that involves in a variety of biological processes of diseases. Limonium aureum (L.) Hill. is an excellent wild plant resource in northern China, which has potential application value for treating oxidative stress. However, there are few studies that focused on the antioxidant effect and related mechanism of L. aureum. Thus, the present study combining systematic network pharmacology and molecular biology aimed to investigate the antioxidant effects of L. aureum and explore its underlying anti-oxidation mechanisms. First, the antioxidant activity of L. aureum extracts was confirmed by in vitro and intracellular antioxidant assays. Then, a total of 11 bioactive compounds, 102 predicted targets, and 70 antioxidant-related targets were obtained from open source databases. For elucidating the molecular mechanisms of L. aureum, the PPI network and integrated visualization network based on bioinformatics assays were constructed to preliminarily understand the active compounds and related targets. The subsequent enrichment analysis results showed that L. aureum mainly affect the biological processes involving oxidation-reduction process, response to drug, etc., and the interference with these biological processes might be due to the simultaneous influence on multiple signaling pathways, including the HIF-1 and ERBB signaling pathways. Moreover, the mRNA levels of predicted hub genes were measured by qRT-PCR to verify the regulatory effect of L. aureum on them. Collectively, this finding lays a foundation for further elucidating the anti-oxidative damage mechanism of L. aureum and promotes the development of therapeutic drugs for oxidative stress.

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Protective effect of polysaccharides of sea cucumber Acaudina leucoprocta on hydrogen peroxide-induced oxidative injury in RAW264.7 cells

Cited by 28 publications

References 34 publications

Antioxidant Potential of Sulfated Polysaccharides from Padina boryana; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model

Antioxidant Potential of Sulfated Polysaccharides from Padina boryana; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model

Colanic Acid: Biosynthetic Overproduction by Engineering Escherichia coli and Physical Property Characterization

Antioxidant Effects and Potential Molecular Mechanism of Action of Limonium aureum Extract Based on Systematic Network Pharmacology

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