Protective effect of Pterocarpus marsupium bark extracts against cataract through the inhibition of aldose reductase activity in streptozotocin-induced diabetic male albino rats

Abstract

The present study was aimed to investigate the protective effect of bark extracts against cataract in streptozotocin-induced diabetic male albino rats. Aldose reductase is a key enzyme in the intracellular polyol pathway, which plays a major role in the development of diabetic cataract. Rats were divided into five groups as normal control, diabetic control, and diabetic control treated with different concentrations of bark extracts. Presence of major constituents in bark extract was performed by qualitative analysis. Body weight changes, blood glucose, blood insulin, and reduced glutathione (GSH) and aldose reductase mRNA and protein expression were determined. Rat body weight gain was noted following treatment with bark extracts. The blood glucose was reduced up to 36% following treatment with bark extracts. The blood insulin and tissue GSH contents were substantially increased more than 100% in diabetic rats following treatment with extracts. Aldose reductase activity was reduced up to 79.3% in diabetic rats following treatment with extracts., , and of aldose reductase were reduced in the lens tissue homogenate compared to the diabetic control. Aldose reductase mRNA and protein expression were reduced more than 50% following treatment with extracts. Treatment with bark was able to normalize these levels. Taking all these data together, it is concluded that the use of bark extracts could be the potential therapeutic approach for the reduction of aldose reductase against diabetic cataract.

Protective Effect of Rutaecarpine in Streptozotocin-Induced Diabetes Cataracts Through the Inhibition of Aldose Reductase Activity in Male Albino Rats

“…Hyperglycemia can produce reactive oxygen species through a variety of processes, including raised an increase in advanced glycation end-products as well as activation of the polyol pathway, overproduction of mitochondrial superoxide and altered activity of protein kinase C activity 36,37 . Rutaecarpine can quench ROS while also increasing the expression of the antioxidant enzymes 38 . The anti-cataract activity of rutaecarpine appears to be effective because of the reduction in oxidative destruction in proteins of the lens and the exact mode of action is unknown.…”

Antidiabetic activity of standardized dried tubers extract of Aconitum napellus in streptozotocin‐induced diabetic rats

“…The antidiabetic activity of chloroform extract of Aconitum napellus was performed in rat models (Das et al 2019;Naz et al 2019). Streptozotocin (STZ) is frequently used drug to induce diabetes in animal models and hence STZ was used in the present work to induce diabetes in rats (Simon et al, 2018;Xu et al, 2018). STZ at the dose of 60 mg/kg i.p.…”

Pterocarpus Marsupium for the Treatment of Diabetes and Other Disorders

“…The protective effect of Pterocarpus marsupium bark extracts against cataract in streptozotocin-induced diabetic male albino rats was investigated by Xu et al [24]. The blood glucose was reduced up to 36% following treatment with bark extracts.…”