Protective effect of Pyropia yezoensis glycoprotein on chronic ethanol consumption-induced hepatotoxicity in rats

Abstract

The present study investigated the protective effect of Pyropia yezoensis glycoprotein (PYGP) against chronic ethanol consumption‑mediated hepatotoxicity in rats. Male Sprague-Dawley rats (n=20; 6 weeks old) were randomly divided into four groups. The rats in each group were treated for 30 days with the following: i) CON group, distilled water only; ii) EtOH group, 20% ethanol 3.7 g/kg/BW; iii) EtOH+150 group, 20% ethanol 3.7 g/kg/BW+PYGP 150 mg/kg/BW; iv) EtOH+300 group, 20% ethanol 3.7 g/kg/BW+PYGP 300 mg/kg/BW. EtOH, PYGP and water were orally administered. The rats were sacrificed after 30 days, and blood and liver samples were collected for analysis. Treatment with ethanol caused significant elevation of serum levels of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT). Furthermore, inhibition of the antioxidant defense system in the liver, including glutathione (GSH), glutathione peroxidase (GSH‑px) and catalase (CAT) was observed. However, co‑administration with PYGP recovered the antioxidant defense system, and the serum levels of GOT and GPT. PYGP was shown to attenuate ethanol toxicity via the inactivation of mitogen‑activated protein kinases (MAKPs). PYGP suppressed the overexpression of cytochrome P450 2E1 (CYP2E1), inducible nitric oxide synthase and cyclooxygenase‑2. These results suggested that the protective effect of PYGP was associated with antioxidant activities, MAPKs and the CYP2E1 signaling pathway.

Phytochemical screening and hepatoprotective potential of Niphidium albopunctatissimum Lellinger on alcoholic hepatoxicity induced in albino rats

“…It serves as a fairly specific indicator of liver status (16) . The decrease in serum GPT values found in our study is consistent with those reported in other investigations with hepatoprotective medicinal plants (17,18) , especially the fact that pretreatment with the extract may be effective in reducing serum GPT before drinking alcohol (17) . The hepatotoxic action of ethanol in control group is evident, since the absorbed ethanol performs its biotransformation process mainly in the liver, at a rate of 10 mL hour (19) ; its metabolism is mainly carried out by the enzymes alcohol dehydrogenase (ADH), aldehyde dehydrogenase (ALDH) and cytochrome P450 (CYP2E1) (20) .…”

Health Functionality and Quality Control of Laver (Porphyra, Pyropia): Current Issues and Future Perspectives as an Edible Seaweed

“…Anti-cancer polysaccharides (dietary fiber, porphyran), phospholipids, sterol, peptide [17,[46][47][48][49][50][51][52][53][54] Prevention of cardiovascular disease (e.g., hypertension, atherosclerosis, ischemia) betaine, dietary fiber, taurine, porphyran [17,[55][56][57][58][59][60][61] Antioxidant effect (e.g., Anti-ageing) porphyran, glycoprotein, polyphenols, tocopherols, peptide [62][63][64][65][66][67] Anti-inflammatory effect and immunomodulation glycoprotein, porphyran [64,[68][69][70][71][72][73] Alcohol metabolism glycoprotein [74,75] Prevention of nervous diseases (e.g., Alzheimer's diseases, methylmalonic acidemias) taurine, porphyran [38,76,77] Prevention of bone disease (e.g., osteoporosis, rheumatoid arthritis) porphyran, glycoprotein [64,78] Anti-diabetes mellitus phenolic compounds (carotenoids, anthocyanins), polysaccharides (porphyran), peptide [79][80][81][82] Porphyran is the distinctive dietary fiber found in laver, and its health effects were intensively studied to determine the nutritional/functional quality of ...…”

Pyropia yezoensis Protein Prevents Dexamethasone-Induced Myotube Atrophy in C2C12 Myotubes

“…Moreover, it exerts various physiological effects, including anti-tumorigenic [22], anti-inflammatory [23], anti-photoaging [24], and anti-oxidant effects [25]. Previous studies reported that glycoproteins derived from P. yezoensis help reduce inflammatory stress induced by lipopolysaccharides in RAW264.7 cells and hepatotoxicity induced by ethanol in a rat model [23,26]. Moreover, P. yezoensis glycoproteins prevented cytotoxicity induced by D-galactosamine in Hepa 1c1c7 cells and acetaminophen-induced liver injury in a rat model [27,28].…”