Protective effect of the methanolic extract of malva parviflora l. leaves on acetic acid-induced ulcerative colitis in rats

Abstract

Background/Aims:Inflammatory bowel disease (IBD) is a general term describing chronic, idiopathic relapsing, inflammatory conditions of the gastrointestinal tract of unknown etiology. Previous studies have indicated that Malva parviflora leaf extract possesses anti-inflammatory, antioxidant, and antiulcerogenic activity. activity. This work aimed to investigatee the anti-inflammatory effect of the methanolic (MEMP) and aqueous (AEMP) extracts of M. parviflora leaves on acetic acid-induced colitis in rats.Materials and Methods:42 male Wistar albino rats were divided into seven groups (n = 6). Group I: Normal saline control group with no colitis; Group II: Acetic acid colitis group; Group III: 100 mg/kg/5 d MEMP; Group IV: 200 mg/kg/5 d.MEMP; Group V: 100 mg/kg/5 d AEMP; Group VI: 200 mg/kg/5 d AEMP; Group VII: Prednisolone group (2 mg/kg/5 d). Treatments were followed by induction of colitis using intrarectal instillation of 2 mL of 4% acetic acid. Colon damage was evaluated macroscopically (spleen weight/body weight, colon weight/length ratio) and the histological changes were also recorded.Results:The results of this study showed that acetic acid caused severe inflammation of the colon and a significant increase in spleen weight/body weight, and an increase in colon weight/length ratio compared with normal control group. Pretreatment with MEMP and AEMP for 5 days followed by induction of colitis resulted in a significant attenuation of spleen weight and colon weight/length ratio compared with acetic acid control group. Methanolic extract provided better anticolitic effect than aqueous extract; the effect was prominent at the dose of 200 mg/kg. Histopathological findings confirmed the protective effect of the MEMP.Conclusion:In conclusion, MEMP could ameliorate mucosal damage in experimentally induced colitis when given orally.

Anti-Inflammatory Effects of Protein Kinase Inhibitor Pyrrol Derivate

“…Chronic UC was induced as described [15]: animals were induced by 2 rectal applications of 4% acetic acid (1 mL) weekly. After administration, the rats were held upside down for approximately 30 seconds to prevent immediate leakage of the agent from the anus.…”

Cardioprotective effect of Malva verticillata against doxorubicin -induced toxicity in rats

“…Antioxidants are suggested to beneficially interfere with diseases or drugs-related oxidative stress [44]. M. verticillata contains a higher total content of flavonoids compared with phenolic components according to the previous studies [9,45]. naturally existing in some plants, and abundantly in M. verticillata [46].…”

Acute and Chronic Antihypertensive Effect of Fractions, Tiliroside and Scopoletin from <i>Malva parviflora</i>

“…8,9) In Mexico this plant is distributed all over the country and the ethnobotanical uses are diverse, it is used for treating wounds, bruises, abscesses, fever, headaches, cold sores, infections and for renal problems. 10) This plant has not been widely studied, some pharma-cological effects are attributed to it such as, anti-inflammatory activity on cyclooxygenase-1 and -2 (COX-1 and COX-2) and other studies demonstrated that the extracts inhibited ear oedema induced with croton oil, 8) as well as, inhibiting the vascular permeability induced with acetic acid, also presenting in a dose dependent form a strong scavenging activity against free radical 2,2-diphenyl-1-picrilhidrazil (DPPH). 9) Methanol extract of M. parviflora also reduces mucosal damage in an experimental colitis assay.…”

“…12) Even though M. parviflora is widely used in traditional medicine and pharmacological studies have been made, the chemical composition has not been completely identified; only some fatty acids: malvalic, sterculic and vernolic acids, as well as, some anthocyanins and phenolic compounds have been reported. 10,13) In this work were evaluated the anti-hypertensive activity of extracts and fractions from M. parviflora by using a chronic model of hypertension induced by angiotensin II (AGII), and in this same assay was measured the capacity of the plant for to modified the concentration of cytokines in kidneys, as well as the lipid peroxidation. And the other hand, was made a chemical separation of the most active fraction and two compounds were isolated by chromatographic column, these substances were tested in an acute hypertension-induced with AGII in mice.…”