Protective effect of theophylline on renal functions in experimental pneumoperitoneum model

Abstract

Our objective in this experimental study is to research the effect of the intra-abdominal pressure which rises following pneumoperitoneum and whether Theophylline has a possible protective activity on this situation. In our study, 24 Wistar Albino rats were used. Rats were divided into two groups. The first group was set for only pneumoperitoneum model. The second group was given 15 mg/kg of Theophylline intraperitoneally before setting pneumoperitoneum model. Then urea, creatinine, cystatin-C, tissue and serum total antioxidant capacity, total oxidant capacity and oxidative stress index in two groups were measured and compared with each other. Apoptosis and histopathological conditions in the renal tissues were examined. The differences between the groups were analyzed with the Mann-Whitney U test. Results were considered significant at p50.05. No statistically significant difference was determined between tissue and serum averages in two groups in terms of TAS, TOS and OSI values (p40.05). The mean value of urea were similar in pneumoperitoneum and pneumoperitoneum + theophylline groups (p ¼ 0.12). The mean cystatin-C value was 2.2 ± 0.3 mg/mL in pneumoperitoneum, 1.74 ± 0.33 mg/mL in pneumoperitoneum + theophylline (p ¼ 0.002). According to our study, lower cystatin-C levels in the group, where Theophylline was given, are suggestive of lower renal injury in this group. However, this opinion is interrogated as there is no difference in terms of tissue and serum TAS, TOS, OSI and urea values between the groups.

Clinical Utility of Serum Cystatin C in Predicting Diabetic Nephropathy Among Patients with Diabetes Mellitus: a Meta-Analysis

“…Arun O showed that serum cystatin C values may indicate acute kidney injury (AKI) signs earlier than blood urea nitrogen and serum creatinine in DM patients undergoing Coronary Artery Bypass Graft Surgery [37]. The study by Ozturk SA et al indicated that the lower cystatin C levels in Wistar Albino rats that were given pneumoperitoneum and theophylline are suggestive of lower renal injury [38].…”

Ischemia Modified Albumin as a New Marker for Diagnosis of Early Pregnancy Losses

“…For these reasons, it is very important to have enough antioxidant capacity during pregnancy. Healthy nutrition and avoiding all factors that cause oxidative stress will provide a more positive pregnancy process [21][22][23][24][25][26][27][28][29][30][31][32] .…”

Hydrogen protects against liver injury during CO2 pneumoperitoneum in rats

“…Some methods are available to reduce pneumoperitoneum-induced injury such as ischemic-preconditioning, pneumoperitoneum preconditioning, low intra-abdominal pressure, stepwise rising CO 2 insufflation, melatonin, theophylline, erythropoietin and dexmedetomidine [ 65 – 71 ]. Some agents are also available to reduce ischemia-reperfusion injury such as epigallocatechin-3-gallate, gastrodin, eupatilin and oxymatrine [ 38 , 72 – 74 ].…”