Protective Effect of Turmeric against Bisphenol-A Induced Genotoxicity in Rats

Abstract

In this study, the protective role of turmeric on genotoxic effects of Bisphenol-A exposure in Wistar rats by in vivo experiment were investigated. Bisphenol-A is a known endocrine disruptor and suspected carcinogen, that comes diet through plastics for food packaging and food processing. In this study, rats were divided into three groups of twelve animals each and were administered with Bisphenol-A by oral gavage with levels of 0, 50 and, 100 mg. Half of the animals in each group were fed with feed which contained 3% turmeric (wt/wt), for a period of 4 wk, while the rest of the rats received the same diet treatment without the addition of turmeric. At the end of the experiment, all rats were terminated and the internal organs such as liver, kidney, femurs were collected and analyzed. Mean and SD values were compared by one-way ANOVA and Kruskal-Wallis-Wilcoxon test, the formation of micronuclei was compared using Mann-Whitney U-test. Significant decrease in serum malondialdehyde and urinary 8-hydroxy-2′-deoxyguanosine levels were observed in Bisphenol-A1 turmeric groups as compared to Bisphenol-A groups. Bisphenol-A groups exhibited significantly higher mean levels of DNA damage in liver and kidney as compared to the untreated control group. Bisphenol-A group showed significant increase in the formation of micronuclei which was approximately threefold higher as compared to the control group. A significant decrease in DNA migration was observed in Bisphenol-A1 turmeric fed groups in liver and kidney. Turmeric feeding significantly inhibited the micronuclei formation induced by Bisphenol-A. The study results indicate that turmeric can protect against Bisphenol-A induced genotoxicity in rats.

Fertaric acid amends bisphenol A-induced toxicity, DNA breakdown, and histopathological changes in the liver, kidney, and testis

“…The very low concentrations of BPA caused toxic effects via affecting the physiological and biochemical parameters in multiple tissues of fish. Also, Panpatil et al [ 68 ] found that the BPA-treated groups exhibited significantly higher mean levels of damage in the liver and kidney as compared to the untreated control group. Furthermore, Pan et al [ 69 ] found that BPA declined sperm chromatin integrity while increased damage in mouse spermatogenic cells.…”

Re‐evaluation of the risks to public health related to the presence of bisphenol A (BPA) in foodstuffs

“…After a screening for the reliability and relevance of the results, six studies from four publications, all ranked as of limited relevance, were selected for further consideration (Figure 3 and Table 15 ). Of these, three studies were considered positive for the induction of MN and CA in the same publication (Tiwari et al, 2012 ) or of MN (Panpatil et al, 2020 [RefID 379‐G]) in rats following daily oral BPA administrations for 6 and 28 days, respectively. Tiwari et al ( 2012 ) applied a range of doses from 2.4 μg up to 50 mg/kg bw per day.…”

“…In a separate publication, the same authors (Tiwari and Vanage, 2017 ) reported that these experimental conditions were associated with the induction of lipid peroxidation (malonaldehyde, MDA) and oxidative stress (decreased SOD, CAT, GSH) in rat bone marrow and peripheral blood lymphocytes. In Panpatil et al ( 2020 ) [RefID 379‐G], the dose range was much lower (50 and 100 μg/kg bw per day). A fourth study tested positive in the mouse bone marrow MN test after the administration of a daily dose of 50 mg/kg bw for 28 days in the presence of high level of cytotoxicity (Fawzy et al, 2018 [RefID 270‐G]).…”

“…In contrast, dose‐related increases in DNA strand breaks were reported at doses greater than 10 μg/kg bw in rats treated for 6 days with a range of doses between 2.4 μg and 50 mg/kg bw per day (Tiwari et al, 2012 ). A weak and dose‐dependent increase in liver DNA strand breaks was observed at 50 and 100 mg/kg bw per day, whereas the increase in kidney was limited to 50 μg/kg bw (Panpatil et al, 2020 [RefID 379‐G]). Finally, in a study on BPA neurotoxicity, a significant increase of strand breaks in brain cells was observed after treatment in a range of doses from 0.5 to 5000 μg/kg bw per day for 8 weeks (Zhou et al., 2017c [RefID 9083]).…”

Natural Products in Mitigation of Bisphenol A Toxicity: Future Therapeutic Use

“…A previous study that investigated the protective effects of turmeric against BPA exposure-induced genotoxicity in Wistar NIN rats revealed that turmeric treatment decreased serum malondialdehyde and urinary 8-hydroxy-2′-deoxyguanosine levels. In addition, turmeric co-administration decreased the formation of micronuclei and DNA migration in hepatic and renal tissues [ 342 ]. Curcumin has been shown to reduce BPA-induced oxidative stress and histopathological alterations in Wistar rats’ testis and cardiac tissues [ 343 , 344 ].…”