Protective effect of Xin Mai Jia ultrafiltration extract on human umbilical vein endothelial cell injury induced by hydrogen peroxide and the effect on the NO-cGMP signaling pathway

Yin, Yulong; Wan, Jia; Li, Peng; Jia, Yanjie; Sun, Ruili; Pan, Guopin; G, Wan

doi:10.3892/etm.2014.1700

Cited by 6 publications

(5 citation statements)

References 25 publications

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“…1D and E , incubation of HUVECs with H 2 O 2 dramatically reduced NO productions and damaged cell viabilities. However, XMJ concentration-dependently reversed NO generations and cell proliferations, consistent with our previous report 19 .…”

Section: Resultssupporting

confidence: 93%

“…By using gene expression array, we identified several signaling pathways related to NO, FOXO, apoptosis, cell cycle, AMPK, MAPK, NF-kB, and PPAR, which were affected by XMJ in endothelial cells. We choose NO signaling as a candidate because NO signaling was in the pecking order among these signaling, consistent with our previous studies that XMJ produces the anti-inflammatory and antioxidant effects in HUVECs by increasing NO productions 19 26 27 . This is also highly related to clinical investigations that Chinese medicine has globally recognized to improve health conditions by activating NO signaling 28 .…”

Section: Discussionmentioning

confidence: 91%

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Traditional Chinese medicine xin-mai-jia recouples endothelial nitric oxide synthase to prevent atherosclerosis in vivo

Yin

Zhu

Wan

et al. 2017

Sci Rep

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Endothelial dysfunction, which is caused by endothelial nitric oxide synthase (eNOS) uncoupling, is an initial step in atherosclerosis. This study was designed to explore whether Chinese medicine xin-mai-jia (XMJ) recouples eNOS to exert anti-atherosclerotic effects. Pretreatment of XMJ (25, 50, 100 μg/ml) for 30 minutes concentration-dependently activated eNOS, improved cell viabilities, increased NO generations, and reduced ROS productions in human umbilical vein endothelial cells incubated with H2O2 for 2 hours, accompanied with restoration of BH4. Importantly, these protective effects produced by XMJ were abolished by eNOS inhibitor L-NAME or specific eNOS siRNA in H2O2-treated cells. In ex vivo experiments, exposure of isolated aortic rings from rats to H2O2 for 6 hours dramatically impaired acetylcholine-induced vasorelaxation, reduced NO levels and increased ROS productions, which were ablated by XMJ in concentration-dependent manner. In vivo analysis indicated that administration of XMJ (0.6, 2.0, 6.0 g/kg/d) for 12 weeks remarkably recoupled eNOS and reduced the size of carotid atherosclerotic plaque in rats feeding with high fat diet plus balloon injury. In conclusion, XMJ recouples eNOS to prevent the growth of atherosclerosis in rats. Clinically, XMJ is potentially considered as a medicine to treat patients with atherosclerosis.

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Section: Resultssupporting

confidence: 93%

Section: Discussionmentioning

confidence: 91%

Traditional Chinese medicine xin-mai-jia recouples endothelial nitric oxide synthase to prevent atherosclerosis in vivo

Yin

Zhu

Wan

et al. 2017

Sci Rep

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“…Excessive levels of ROS in endothelial cells leads to impaired endothelial nitric oxide (NO) bioactivity (7,8). NO, a type of vasodilator, is secreted by endothelial cells, and 30% of blood NO levels are produced by endothelial nitric oxide synthase (9). Previous studies have reported that increased ROS generation in endothelial cells contributes to their dysfunction in hypertension (10)(11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

Isolariciresinol-9'-O-α-L-arabinofuranoside protects against hydrogen peroxide‑induced apoptosis of human umbilical vein endothelial cells via a PI3K/Akt/Bad‑dependent pathway

Liu¹,

Liu

Wang

et al. 2017

Mol Med Report

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Isolariciresinol-9'-O-α-L-arabinofuranoside (MWS‑19) isolated from Pinus massoniana Lamb. Fresh pine needles is the major ingredient of the Songling Xuemaikang capsule therapy used for hypertension. The present study aimed to investigate the effects and underlying mechanisms of MWS‑19 on hydrogen peroxide (H2O2)‑induced apoptosis in human umbilical vein endothelial cells (HUVECs). To investigate the effect of MWS‑19 on apoptosis in HUVECs, an oxidative stress‑induced apoptosis model was established in HUVECs using H2O2, and the present study performed Hoechst 33258 staining and a Cell Counting Kit‑8 (CCK‑8) assay. Furthermore, western blot analysis was also performed to investigate the underlying mechanism of the effects of MWS‑19 on the model. The results demonstrated that MWS‑19 reversed the effects of H2O2 on cell apoptosis at a concentration range of 15.6‑250 µg/ml, with dose‑dependent increases in cell growth. Hoechst staining indicated that 500 µM H2O2 induced HUVEC apoptosis, and MWS‑19 markedly protected HUVECs against apoptosis at 31.3, 62.5 and 125 µg/ml. Furthermore, the protein expression of phosphatidylinositol 3‑kinase (PI3K), phosphorylated‑Akt and Bcl‑2‑associated agonist of cell death (Bad) were increased, and reduced caspase‑3 activation was observed, following treatment with MWS‑19 in H2O2‑treated HUVECs. Additionally, the PI3K inhibitor wortmannin attenuated PI3K/Akt/Bad signaling induced by MWS‑19 treatment and neutralized the effect of MWS‑19 on the growth of HUVECs. In conclusion, the results of the present study indicate that MWS‑19 may protect against H2O2‑induced HUVEC apoptosis via the PI3K/Akt/Bad signaling pathway. MWS‑19 may serve an important role in the prevention of oxidative damage in vascular endothelial cells in hypertension patients.

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“…[ 9 10 11 12 ] The previous studies of our group showed that Gegen and its TCM preparation XinMaiJia could effectively inhibit the occurrence and development of atherosclerosis. [ 13 14 15 16 ]…”

Section: Introductionmentioning

confidence: 99%

Rapid determination of puerarin by near-infrared spectroscopy during percolation and concentration process of puerariae lobatae radix

et al. 2016

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Background:Gegen (Puerariae Labatae Radix) is one of the important medicines in Traditional Chinese Medicine. The studies showed that Gegen and its preparation had effective actions for atherosclerosis.Objective:Near-infrared (NIR) was used to develop a method for rapid determination of puerarin during percolation and concentration process of Gegen.Materials and Methods:About ten batches of samples were collected with high-performance liquid chromatography analysis values as reference, calibration models are generated by partial least-squares (PLS) regression as linear regression, and artificial neural networks (ANN) as nonlinear regression.Results:The root mean square error of prediction for the PLS and ANN model was 0.0396 and 0.0365 and correlation coefficients (r2) was 97.79% and 98.47%, respectively.Conclusions:The NIR model for the rapid analysis of puerarin can be used for on-line quality control in the percolation and concentration process.SUMMARY Near-infrared was used to develop a method for on-line quality control in the percolation and concentration process of GegenCalibration models are generated by partial least-squares (PLS) regression as linear regression and artificial neural networks (ANN) as non-linear regressionThe root mean square error of prediction for the PLS and ANN model was 0.0396 and 0.0365 and correlation coefficients (r2) was 97.79% and 98.47%, respectively. Abbreviations used: NIR: Near-Infrared Spectroscopy; Gegen: Puerariae Loabatae Radix; TCM: Traditional Chinese Medicine; PLS: Partial least-squares; ANN: Artificial neural networks; RMSEP: Root mean square error of validation; R2: Correlation coefficients; PAT: Process analytical technology; FDA: The Food and Drug Administration; Rcal: Calibration set; RMSECV: Root mean square errors of cross-validation; RPD: Residual predictive deviation; SLS: Straight Line Subtraction; MLP: Multi-Layer Perceptron; MSE: Mean square error.

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Protective effect of Xin Mai Jia ultrafiltration extract on human umbilical vein endothelial cell injury induced by hydrogen peroxide and the effect on the NO-cGMP signaling pathway

Cited by 6 publications

References 25 publications

Traditional Chinese medicine xin-mai-jia recouples endothelial nitric oxide synthase to prevent atherosclerosis in vivo

Traditional Chinese medicine xin-mai-jia recouples endothelial nitric oxide synthase to prevent atherosclerosis in vivo

Isolariciresinol-9'-O-α-L-arabinofuranoside protects against hydrogen peroxide‑induced apoptosis of human umbilical vein endothelial cells via a PI3K/Akt/Bad‑dependent pathway

Rapid determination of puerarin by near-infrared spectroscopy during percolation and concentration process of puerariae lobatae radix

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