Protective effect of zinc against cadmium toxicity on pregnant rats and their fetuses at morphological, physiological and molecular level

El-Sayed, Ashraf S. A.; Salem, Salem M.; El-Garhy, Amany A.; Rahman, Zeinab A.; Kandil, Asmaa M.

doi:10.5897/ajb12.2829

Cited by 10 publications

(1 citation statement)

References 54 publications

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“…Industrial development has brought man into contact with several persistent chemicals, including heavy metals, such as cadmium (Cd) [1]. Cd is a heavy metal with great environmental health effects and all Cd compounds have been classified as human carcinogens.…”

Section: Introductionmentioning

confidence: 99%

The Role of Urinary Cadmium on Liver Function and Erythrocytes Cell Count in Pregnancy

Wibowo¹,

Rahaju²,

Iskandar³

et al. 2014

IJBBB

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Abstract-Cadmium (Cd) is a heavy metal, which is used in many industries and was confirmed as an environmental toxin and mainly effects haematological system and liver. Previous studies showed that Cd induced damage of the liver and influenced erythrocytes. The effect of Cd in haematological system and liver function especially in pregant women has not been much studied. Thus our study aimed to evaluate the potential effect of cadmium exposure on erythrocytes and liver function in pregnant women by measuring their correlation on the number of erythrocytes and the levels of Aspartate Transaminase (AST) and Alanine Transmainase (ALT). This study was performed between July to December 2013 and targeted 58 pregnant women who were admitted for treatment and termination of pregnancy due to medical or obstetrical indications in Mutiara Bunda Mother and Child Hospital, Martapura, South Kalimantan, Indoneseia. For analyzing of the data, SPSS software version 17 was used and was examined by Pearson correlation test. We found that Cd has a negative correlation with erythroctes cell count and ALT levels and a positive correlation with AST levels, but the three correlations are not significant on statistical analysis. It can be concluded from our results Cd caused erythrocytes cells and tissue damage but did not cause liver damage.Index Terms-Alanine transaminase, aspartate transmainase, cadmium, erythrocytes, pregnant women.

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Section: Introductionmentioning

confidence: 99%

The Role of Urinary Cadmium on Liver Function and Erythrocytes Cell Count in Pregnancy

Wibowo¹,

Rahaju²,

Iskandar³

et al. 2014

IJBBB

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Protective Effects of Ascorbic Acid Against Cadmium‐Induced Toxicity in the Placenta and Fetus of Rats

Başeğmez,

Karakaya,

Kan

et al. 2024

J Biochem & Molecular Tox

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This study aimed to determine the protective role of l‐ascorbic acid in a pregnant rat model of cadmium‐induced toxicity. Cadmium is a toxic heavy metal that can seriously harm placenta and fetus tissue in pregnant women. Forty‐two healthy female Wistar albino rats (250–300 g weight and 14–16 weeks) were randomly distributed into six equal groups (n = 7): control, cadmium 1 mg (CD1), cadmium 5 mg (CD5), ascorbic acid (AA), CD1+AA, CD5+AA. Cadmium was administered to pregnant rats by oral gavage every other day, and/or AA (200 mg) was administered every day. At the end of pregnancy (Day 21), blood, placenta, and fetuses were collected from rats. The results indicated that cadmium‐induced oxidative stress by increasing the level of MDA and by decreasing the levels of GSH, SOD, and CAT activity in the serum of maternal. However, AA administration significantly decreased MDA levels and increased GSH levels, SOD, and CAT activity (p < 0.05). Cadmium (5 mg/kg) exposure significantly increased creatinine levels compared to AA and CD1+AA groups (p < 0.05). In addition, AA (200 mg/kg) significantly attenuated cadmium‐induced histopathological alteration in the placental and fetal tissues. In conclusion, AA may prevent cadmium toxicity in maternal and fetal tissues, as it regulates oxidative imbalance in pregnant rat tissues and alleviates histopathological changes.

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Effect of zinc chloride and sodium selenite supplementation on in vitro maturation, oxidative biomarkers, and gene expression in buffalo (Bubalus bubalis) oocytes

Khalil

Yang

El-Moghazy

et al. 2021

Zygote

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Summary This study examined the effects of zinc chloride (ZnCl2) and sodium selenite (Na2SeO3) supplementation in maturation medium on in vitro maturation (IVM) rate, oxidative biomarkers and gene expression in buffalo oocytes. Ovaries from a slaughterhouse were aspirated and good quality cumulus–oocyte complexes (COCs) with at least four layers of compact cumulus cells and evenly granulated dark ooplasm were selected. COCs were randomly allocated during IVM (22 h) to one of four treatment groups: (1) control maturation medium (basic medium), or basic medium supplemented with (2) ZnCl2 (1.5 µg/ml), (3) Na2SeO3 (5 µg/l), or (4) ZnCl2 + Na2SeO3 (1.5 µg/ml + 5 µg/l, respectively). Oocytes were denuded after 22 h of IVM in the first four replicates. Specimens were fixed and stained to evaluate the stage of nuclear maturation. The spent medium was collected for biochemical assays of total antioxidant capacity (TAC), malondialdehyde (MDA) and hydrogen peroxide concentrations. A second four replicates were used for COCs for RNA extraction. The expression levels of antioxidant (SOD1, GPX4, CAT and PRDX1), antiapoptotic (BCL2 and BCL-XL) and proapoptotic (BAX and BID) genes were measured. Supplementation with ZnCl2 and Na2SeO3 during IVM increased the ratio of oocytes reaching metaphase II at 22 h, increased TAC and decreased MDA and H2O2 concentrations in the maturation medium (P < 0.05). Moreover, beneficial effects were associated with complementary changes in expression patterns of antioxidative, antiapoptotic and proapoptotic genes, suggesting lower oxidative stress and apoptosis. Supplementation medium with zinc chloride and sodium selenite improves the maturation rate, reduces oxidative stress and increases expression levels of antioxidative and antiapoptotic genes.

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Protective effect of zinc against cadmium toxicity on pregnant rats and their fetuses at morphological, physiological and molecular level

Cited by 10 publications

References 54 publications

The Role of Urinary Cadmium on Liver Function and Erythrocytes Cell Count in Pregnancy

The Role of Urinary Cadmium on Liver Function and Erythrocytes Cell Count in Pregnancy

Protective Effects of Ascorbic Acid Against Cadmium‐Induced Toxicity in the Placenta and Fetus of Rats

Effect of zinc chloride and sodium selenite supplementation on in vitro maturation, oxidative biomarkers, and gene expression in buffalo (Bubalus bubalis) oocytes

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