Protective Effects of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-d-glucoside on Ovariectomy Induced Osteoporosis Mouse Model

Abstract

2,3,5,4′-Tetrahydroxystilbene-2-O-β-d-glucoside (TSG), an active polyphenolic component of Polygonum multiflorum, exhibits many pharmacological activities including antioxidant, anti-inflammation, and anti-aging effects. A previous study demonstrated that TSG protected MC3T3-E1 cells from hydrogen peroxide (H2O2) induced cell damage and the inhibition of osteoblastic differentiation. However, no studies have investigated the prevention of ovariectomy-induced bone loss in mice. Therefore, we investigated the effects of TSG on bone loss in ovariectomized mice (OVX). Treatment with TSG (1 and 3 μg/g; i.p.) for six weeks positively affected body weight, uterine weight, organ weight, bone length, and weight change because of estrogen deficiency. The levels of the serum biochemical markers of calcium (Ca), inorganic phosphorus (IP), alkaline phosphatase (ALP), and total cholesterol (TCHO) decreased in the TSG-treated mice when compared with the OVX mice. Additionally, the serum bone alkaline phosphatase (BALP) levels in the TSG-treated OVX mice were significantly increased compared with the OVX mice, while the tartrate-resistant acid phosphatase (TRAP) activity was significantly reduced. Furthermore, the OVX mice treated with TSG showed a significantly reduced bone loss compared to the untreated OVX mice upon micro-computed tomography (CT) analysis. Consequently, bone destruction in osteoporotic mice as a result of ovariectomy was inhibited by the administration of TSG. These findings indicate that TSG effectively prevents bone loss in OVX mice; therefore, it can be considered as a potential therapeutic for the treatment of postmenopausal osteoporosis.

Heshouwu (Polygonum multiflorum Thunb.) Extract Attenuates Bone Loss in Diabetic Mice

“…Diabetic patients have a higher risk of bone fractures (Kanazawa, 2015). Because our coworkers previously indicated that HSW hot water extract and its bioactive component THSG prevent bone loss in an ovariectomy-induced osteoporosis mouse model (Hwang et al, 2016; Kim et al, 2018b), this study was conducted to determine if HSW extract may prevent bone loss caused by diabetes.…”

“…We previously showed that Heshouwu ethanol extract attenuates hyperglycemia and hyperinsulinemia in diet-induced obese mice (Choi et al, 2018). Hwang et al (2016) and Kim et al (2018b) reported that Heshouwu hot water extract and tetrahydroxystilbene glucoside (THSG), which is a bioactive compound of Heshouwu, prevent bone loss in estrogen deficient mice. Zhang et al (2019) recently reported that THSG protects against diabetes-induced osteoporosis in mice with streptozotocin (STZ)-induced diabetes.…”

Restorative effect of modified dioscorea pills on the structure of hippocampal neurovascular unit in an animal model of chronic cerebral hypoperfusion

“…Through prompt the biosynthesis of enzymes relating to mitochondrial energy conversion, Dioscorea optimizes energy metabolism under hypoxia and resists apoptosis to keep cell “young” [33]. Component of Polygonum multiflorum has many pharmacological activities including antioxidant, anti-inflammation, and anti-aging effects [34]. Codonopsis pilosula can acts as an immunoregulator which inhibits too strong inflammation to weaken apoptosis and gliosis [35].…”

The Protective Effects of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-d-Glucoside in the OVA-Induced Asthma Mice Model

“…From this viewpoint, asthma treatment using PM extract has the potential to cause various side effects. Nevertheless, PM has various biological effects [13,14,15,16] and 2,3,5,4′-tetrahydroxystilbene-2-O-β- d -glucoside (TSG) as a main single compound has anti-inflammatory effects [14] and anti-osteoporosis effect [13]. In the present study, we investigated the effects of TSG on asthma as one of the inflammatory diseases.…”

“…Pharmacological studies have emphasized the benefits of PM in the treatment of a variety of diseases, such as cancer, diabetes, alopecia, atherosclerosis, and neurodegenerative diseases [11]. 2,3,5,4′-tetrahydroxystilbene-2-O-β- d -glucoside (TSG) is known as a major active single compound of PM [12], and presents a variety of biological effects, such as anti-osteoporosis [13], anti-inflammation [14], kidney protection [15], anti-cancer [16], and liver protection effects [17]. However, Lei et al reported that PM extracts have hepatotoxicity [18], and this is probably due to the cis isomer of TSG [19].…”