Protective Effects of 2′,4′-Dihydroxy-6′-methoxy-3′,5′-dimethylchalcone to PC12 Cells against Cytotoxicity Induced by Hydrogen Peroxide

Abstract

Oxidative stress has been considered as a major cause of cellular injuries in various clinical abnormalities. One of the possible ways to prevent reactive oxygen species (ROS)-mediated cellular injury is dietary or pharmaceutical therapies to augment the endogenous antioxidant defense capacity. The present study found that 2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone (DMC), a chalcone isolated from the buds of Cleistocalyx operculatus, possessed cytoprotective activity in PC12 cells treated with H(2)O(2). The results showed that DMC could effectively increase cell viability [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) reduction], decrease the cell apoptotic percentage [annexin V/propidium iodide (AV/PI) assay], prevent the membrane from damage [lactate dehydrogenase (LDH) release], scavenge ROS formation, reduce caspase-3 activity, and attenuate the decrease of mitochondrial membrane potential (MMP) in PC12 cells treated with H(2)O(2). Meanwhile, DMC increased the catalytic activity of superoxide dismutase (SOD) and the cellular amount of glutathione (GSH), decreased the cellular amount of malondialdehyde (MDA), and decreased the production of lipid peroxidation in PC12 cells treated with H(2)O(2).

Isolation, Characterization, Crystal Structure Elucidation, and Anticancer Study of Dimethyl Cardamonin, Isolated from Syzygium campanulatum Korth

“…The compound DMC has been isolated from C. operculatus [7], M. serrata [8], and S. samarangense [9]. DMC possessed hepatoprotective [10], cytoprotective [11], anti-inflammatory [12], antifungal, antibacterial [8], anti-TB [13], antiviral [14], antispasmodic [15], antidiarrhoeal [16], antihyperglycaemic [17], anti-MDR [18, 19], and antiapoptotic effect [20]. DMC has been evaluated for its cytotoxic activity on various human cancer cells including liver cancer (SMMC-7721s), HeLa (SPC-A-1, 95-D, and GBC-SD) [7], colon (SW-480) [9], leukemia (K562) [21], colorectal carcinoma (HCT-116 and LOVO) [22], breast (MDA-MB-453), and ovarian [23] cell lines.…”

Iron and Iron Regulatory Proteins in Amoeboid Microglial Cells Are Linked to Oligodendrocyte Death in Hypoxic Neonatal Rat Periventricular White Matter through Production of Proinflammatory Cytokines and Reactive Oxygen/Nitrogen Species

“…ROS causes selective degeneration of oligodendrocytes (Griot et al, 1990) by promoting LPO (Su et al, 2011). Following a hypoxic-ischemic injury, the white matter showed increased levels of MDA ) and 4-HNE, which are toxic to axons and oligodendrocytes (Lin et al, 2004).…”

“…GSH depletion in oligodendrocytes is implicated in their susceptibility to free radical-mediated injury (Back et al, 1998). Activated microglia have been reported to release ROS (Block et al, 2007), which induces LPO (Su et al, 2011). On the basis of these observations, the primary cultured oligodendrocytes were divided into six groups and treated as described below for GSH and LPO measurement.…”

Synthesis, characterization and in vitro photodynamic study of FA-TiO2 nanocomposite

“…Photodynamic FA-TiO 2 could result in subsequent cell damage and a salient feature is the decrease in MMP [33,34], which was monitored by Rh 123 fluorescence. As shown in Fig.…”