Protective effects of an aqueous extract of Welsh onion green leaves on oxidative damage of reactive oxygen and nitrogen species

Wang, Bor-Sen; Lin, Shie-Shin; Hsiao, Wen-Chuan; Fan, Jin-Jia; Fuh, Lih-Fang; Duh, Pin-Der

doi:10.1016/j.foodchem.2005.05.057

Cited by 40 publications

(25 citation statements)

References 47 publications

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“…Inhibition of protein carbonyl formation The eff ect of milk thistle seed extract on protein oxidation was determined using a modifi ed method of Wang et al (22). Bovine serum albumin (BSA; 4 mg/mL) was treated with iron(III) chloride (50 μM), hydrogen peroxide (1 mM) and ascorbic acid (100 μM) in potassium phosphate buff er (20 mM, pH=7.4) in the presence of seed extract (10-1000 μg/mL) at 37 °C for 30 min and then the absorbance was measured at λ=370 nm.…”

Section: Protein Damage Protectionmentioning

confidence: 99%

Assessment of the Antioxidant Activity of Silybum marianum Extract and Its Protective Effect against DNA Oxidation, Protein Damage and Lipid Peroxidation

Serçe¹,

Toptancı²,

Tanrıkut³

et al. 2016

Food Technol. Biotechnol.

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SummaryAntioxidant properties of ethanol extract of Silybum marianum (milk thistle) seeds was investigated. We have also investigated the protein damage activated by oxidative Fenton reaction and its prevention by Silybum marianum seed extract. Antioxidant potential of Silybum marianum seed ethanol extract was measured using diff erent in vitro methods, such as lipid peroxidation, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing power assays. The extract signifi cantly decreased DNA damage caused by hydroxyl radicals. Protein damage induced by hydroxyl radicals was also effi ciently inhibited, which was confi rmed by the presence of protein damage markers, such as protein carbonyl formation and by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The present study shows that milk thistle seeds have good DPPH free radical scavenging activity and can prevent lipid peroxidation. Therefore, Silybum marianum can be used as potentially rich source of antioxidants and food preservatives. The results suggest that the seeds may have potential benefi cial health eff ects providing opportunities to develop value-added products.

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Section: Protein Damage Protectionmentioning

confidence: 99%

Assessment of the Antioxidant Activity of Silybum marianum Extract and Its Protective Effect against DNA Oxidation, Protein Damage and Lipid Peroxidation

Serçe¹,

Toptancı²,

Tanrıkut³

et al. 2016

Food Technol. Biotechnol.

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“…Indeed, Welsh onion possesses antioxidant compounds such as quercetin and kaempferol as main phenol compounds (Wang et al, 2006), ascorbic acid (Kähkönen and Heinonen, 2003), and volatile sulfur compounds (Wu et al, 2001). Thus, it is possible that the content of other antioxidant compounds besides phenol compounds may be increased in the…”

Section: Resultsmentioning

confidence: 99%

“…Welsh onion (Allium fistulosum L.), a member of the Allium family, is a popular vegetable in Asian countries and a reputed good source of phenol compounds with antioxidant activity, leading to beneficial medicinal effects (Helen et al, 2000;Wang et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…However, there are several crops with an inherent capacity to withstand salinity-stress, which allows for stable vegetable production and significantly contributes to palatability and food functionality (Sato et al, 2006). Thus, there have been several reports on the application of salinity-stress for improving the quality of vegetables such as tomato fruits (Auerswald et al, 1999), spinach (Makabe and Tanii, 2008), and strawberry (Keutgen and Pawelzik, 2000).Welsh onion (Allium fistulosum L.), a member of the Allium family, is a popular vegetable in Asian countries and a reputed good source of phenol compounds with antioxidant activity, leading to beneficial medicinal effects (Helen et al, 2000;Wang et al, 2006).Hence, we focused our attention on exploiting salinity-stress treatment for the development of value-added Welsh onion with augmented palatability and food functionality. There are relatively few studies on the salt tolerance of Welsh onion.…”

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confidence: 99%

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Changes in Sugar Content and Antioxidant Activity of Allium Vegetables by Salinity-stress

Arakaki

Takahashi

Hossain

et al. 2014

FSTR

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Three Allium vegetables, Welsh onion from Okinawa, and Wakegi from Okinawa and Nagasaki were subjected to salinity-stress using seawater. The K + /Na + ratio of the three Allium vegetables clearly decreased; however, there was no significant effect on growth parameters. Of the three Allium vegetables, 10% seawater treatment of Welsh onion and Wakegi from Nagasaki clearly enhanced both the sugar content and antioxidant activity. Therefore, seawater treatment may be potentially useful for the development of value-added Allium vegetables, enhancing the palatability and food functionality.Keywords: Allium vegetable, salinity-stress, seawater, sugar content, antioxidant activity IntroductionSalinity is a major environmental factor limiting crop growth and productivity (Rhodales and Loveday, 1990). During the onset and development of salinity-stress within a crop plant, major processes such as photosynthesis, protein synthesis, and energy and lipid metabolism are affected, leading to qualitative and yield losses in most crops (Hasegawa et al., 2000; Hagemann and Erdmann, 1997; Hayashi and Murata 1998). However, there are several crops with an inherent capacity to withstand salinity-stress, which allows for stable vegetable production and significantly contributes to palatability and food functionality (Sato et al., 2006). Thus, there have been several reports on the application of salinity-stress for improving the quality of vegetables such as tomato fruits (Auerswald et al., 1999), spinach (Makabe and Tanii, 2008), and strawberry (Keutgen and Pawelzik, 2000).Welsh onion (Allium fistulosum L.), a member of the Allium family, is a popular vegetable in Asian countries and a reputed good source of phenol compounds with antioxidant activity, leading to beneficial medicinal effects (Helen et al., 2000;Wang et al., 2006).Hence, we focused our attention on exploiting salinity-stress treatment for the development of value-added Welsh onion with augmented palatability and food functionality. There are relatively few studies on the salt tolerance of Welsh onion. In this study, we investigate the effects of artificial salinity-stress on the growth, relative chlorophyll content, and K + /Na + ratio of Allium vegetables: Welsh onion and Wakegi (Allium x wakegi A.), and assess the effect of salinity-stress on sugar content, total phenol content, and antioxidant activity. Materials and MethodsCultivation The bulbs of three types of Japanese Allium cultivars, green-leafy Welsh onion (Okinawa) and two different cultivation lines of Wakegi from Okinawa and Nagasaki (NagasakiWakegi) were used in this study. The upper end of each bulb was cut to adjust the overall length to 5 cm before cultivation. Each bulb type was randomly divided into four test groups of 15 plants each. One group was treated with distilled water (control), and the other three groups were treated with different concentrations of seawater (2% seawater: SW 1; 10% seawater; SW 2; 100% seawater: SW 3) to induce salinity-stress. Each type of bulb was cultivated in a W...

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“…20 The reaction mixture (1.2 mL) containing extract, potassium phosphate buffer (20 mM, pH 7.4), BSA (4 mg), FeCl 3 (50 µM), H 2 O 2 (1 mM) and ascorbic acid (100 µM) were incubated for 60 min at 37 ºC. For determination of PCO content in samples, 1 mL of 10 mM DNPH (2, 4-Dinitrophenylhydrazine) in 2 N HCl was added to the reaction mixture.…”

Section: Inhibitory Activity Of Are On Protein Carbonyl (Pco) Formationmentioning

confidence: 99%

Ashwagandha Root Extract Inhibits Acetylcholine Esterase, Protein Modification and Ameliorates H2O2-Induced Oxidative Stress in Rat Lymphocytes

Pal¹,

Kandikattu²,

Bhushan³

et al. 2017

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Background: Herbs have long been the basis of medical treatment but the practice of herbalism is not strictly based on evidences gathered using scientific methods. Ashwagandha (Withania somnifera L.) is a traditional medicinal herb used in the treatment of various ailments. Objective: In current study we have evaluated the acetylcholine esterase (AChE) inhibitory and antioxidant potential of Ashwagandha root extract (ARE) in in-vitro and ex-vivo models to give a scientific base to its use in herbal medicine. Methodology: Simplest extraction e.g. maceration method was performed for preparation of extracts using methanol as solvent. Both in vitro and in vivo experimental trial were executed to evaluate the efficacy of root extract. Result and Discussion: ARE substantially scavenged free radicals and effectively prevented protein degradation as well as modification as studied by SDS-PAGE and Western blotting. Pre-treatment with ARE protected rat lymphocytes against H 2 O 2 -induced oxidative damage. H 2 O 2 treatment resulted in DNA fragmentation and significantly decreased the activities of key antioxidant enzymes namely superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glucose-6-phosphate dehydrogenase while decreased and increased the content of glutathione and malondialdehyde, respectively. ARE pre-treatment almost reversed these changes indicating its efficiency to suppress hydrogen peroxide-induced oxidative stress. Conclusion: The study provides the scientific basis of pleiotropic functions of Ashwagandha. Key words: Acetylcholine esterase, Rat lymphocytes, Antioxidants, Ashwagandha, Protein carbonylation, DNA damage. the loss of cholinergic innervation, reduction of choline acetyltransferase (ChAT) and enhanced acetylcholine esterase (AChE) activity. AChE terminates the interaction between neurotransmitter acetylcholine (ACh) and the corresponding receptor protein (acetylcholine receptor, nAChR), which is the basis of the intercellular communication in brain. Pharmacological and analytical data from human tissue and body fluids have implicated oxidation products of fatty acids in the pathogenesis of AD. 7 Antioxidants can act by scavenging the reactive products of lipid peroxidation and may be useful in prevention and treatment of AD. The modulation of AChE is presently the most accepted and recognized therapeutic marker for development of cognitive enhancers. 8 Currently used AChE inhibitors, like tacrine, produce side effects such as hepatotoxicity. Ajay9 So, the use of natural bioactive compounds, like antioxidants, finds their possible application in the prevention and treatment of AD. Dastmalchia and his associates have summarized that interest in the discovery of natural antioxidants has risen exponentially, principally for three reasons: 303Determination of antioxidant capacity using ABTS + (2, 2'-azinobis 3-ethylbenzothiazoline-6-sulfonate) assayThe method used was the ABTS + (cation radical) decolorization assay which is based on the ability of an antioxidant ...

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Protective effects of an aqueous extract of Welsh onion green leaves on oxidative damage of reactive oxygen and nitrogen species

Cited by 40 publications

References 47 publications

Assessment of the Antioxidant Activity of Silybum marianum Extract and Its Protective Effect against DNA Oxidation, Protein Damage and Lipid Peroxidation

Assessment of the Antioxidant Activity of Silybum marianum Extract and Its Protective Effect against DNA Oxidation, Protein Damage and Lipid Peroxidation

Changes in Sugar Content and Antioxidant Activity of Allium Vegetables by Salinity-stress

Ashwagandha Root Extract Inhibits Acetylcholine Esterase, Protein Modification and Ameliorates H2O2-Induced Oxidative Stress in Rat Lymphocytes

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