Protective Effects of Bi-Herbal Formulation of Aqueous Extracts of Vernonia amygdalina and Gongronema latifolium against Gentamicin Induced Nephrotoxicity and Liver Injury in Rats

Constance, Nwadike; Joy, Dike-Ndudim; Nkeiruka, Oly-Alawuba; Ahamefula, Ezekwe; Chidimma, Akanazu; Oluchi, Aloy-Amadi

doi:10.9734/ajrb/2020/v7i430144

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…It is indigenous to tropical Africa (Kokwaro 2009) and propagated by seeds and stems cuttings, which grow faster than seedlings. It is cultivated as food and medicines amongst the local people of Nigeria (Ewuro in Yoruba, Chusardoki in Hausa & Onugbu) and Cameroon (Lusoga) (Constance et al 2020). It grows very well under full sunlight and humid environmental conditions.…”

Section: Introductionmentioning

confidence: 99%

Secondary metabolites profiling of Vernonia amygdalina Del. in response to copper-induced abiotic stress

Adu

Ojekale

John

et al. 2021

JOMPED

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Background: Vernonia amygdalina is a green leafy vegetable that grows in tropical Africa and popularly cultivated in the southern part of Nigeria for its economic, nutritional and ethnomedicine value.Aims: This study analysed the influence of copper-induced abiotic stress on the deoxyribonucleic acid and secondary metabolites of V. amygdalina.Setting: Analyses of plant material were carried out in the Department of Botany, Lagos State University.Methods: Deoxyribonucleic acid (DNA) extraction and Inter Simple Sequence Repeat (ISSR) amplification were performed. Copper (Cu) concentration in leaves and stem of V. amygdalina was determined using inductively coupled plasma-optical emission spectroscopy (ICP-OES), secondary metabolites were determined using gas chromatography–mass spectrometry (GC-MS) and the effect on DNA amplification and profiling was analysed using gel electrophoresis.Results: After 41 days of cultivation, the stem of V. amygdalina significantly bioaccumulated 37 mg/L, 50 mg/L and 215 mg/L of Cu after spiking with 400 mg/L, 1000 mg/L and 2000 mg/L, respectively, whilst the leaves accumulated 52 mg/L, 100 mg/L and 350 mg/L of Cu, respectively. Copper-impregnated soil influenced phytochemicals of the plants by causing inconsistent increase and decrease in specific compounds such as levomenthol, methyl stearate and glycerine. Deoxyribonucleic acid bands of the stem and leaf of V. amygdalina from control site showed no band shift, whilst band shift occurred in the stem and leaf of the Cu-spiked V. amygdalina.Conclusion: This study revealed the leaves of V. amygdalina having higher Cu accumulation capacity than the stems. Also, Cu alters the quality and quantity of phytochemicals in plant parts.

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Section: Introductionmentioning

confidence: 99%

Secondary metabolites profiling of Vernonia amygdalina Del. in response to copper-induced abiotic stress

Adu

Ojekale

John

et al. 2021

JOMPED

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“…31,32 Following treatment with the plant extract, the activities of these marker enzymes were significantly reduced in the G. latifolium extract treated diabetic rats (Tables 2 and 3), indicating the plant's hepatoprotective properties, which is consistent with some studies reporting the plant's hepatoprotective properties. 33,34 The ability of G. latifolium to exert a protective effect on the liver and lower the level of liver enzymes in the blood could be related to flavonoids' hepaprotective characteristics, which serve as membrane stabilizers to protect the liver cells from harm. 26 In acute toxicity study, a scale proposed by Lorke (1984) roughly classifies substances administered via the oral route according to their LD50 as follows: Very toxic (LD50 < 1.0 mg/kg bw), toxic (LD50 up to 10.0 mg/kg bw), less toxic (LD50 up to 100.0 mg/kg bw) and only slightly toxic (up to 1000.0 mg/kg bw).…”

Section: Resultsmentioning

confidence: 99%

Biochemical and Hepatoprotective Effects of Gangronema latifolium Extract on Alloxan-Induced Diabetic Rats

2022

TJNPR

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Medicinal plants have provided valuable therapeutic agents for treating diseases and disorders, and they are widely employed in many regions of the world, particularly in rural areas where modern medical facilities are limited. 1 Recently, there has been an increase in plant-based therapeutic products in both developed and developing countries because they are mostly non-toxic, have fewer side effects, and are available at affordable prices. 2,3 One such medicinal plant is Gongronema latifolium. The plant belongs to the family of Asclepiadacae. It is an edible rainforest plant native to many parts of Nigeria and West Africa. 4 The plant is commonly used as a vegetable or spice and also widely been used in traditional medicine for treating diabetes and high blood pressure 5 and malaria. 6 G. latifolium is also used to treat various other ailments such as cough, loss of appetite, parasitic intestinal worms and stomach disorders. The leaf extract was also reported to exhibit antimicrobial properties 7 and anti-diabetic properties, [8][9][10][11] and in the United States, G latifolium leaves are processed into tea and marketed to diabetes mellitus patients. 12 Increasingly, diabetes management involves non-conventional drugs. It is estimated that 25 to 57% of people with diabetes have at one time or another resorted to complementary and alternative medicine, including medicinal plants. 13

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“…Its common name is 'amaranth globe'. In Nigeria, G. latifolium is known by different local names such as 'utasi' by the Efiks/Ibibios, 'utazi' by the Igbos and arokeke by the Yorubas 13,14 . The phytochemical screening of GL reveals that the plant contains flavonoid, polyphenol, saponin, tannin alkaloid and mineral.…”

Section: Introductionmentioning

confidence: 99%

Hepato and Nephro-protective Potentials of Gongronema latifolium in Streptozotocin Induced diabetic Rat Model

Ujong

Beshel

Etah

et al. 2022

J. Drug Delivery Ther.

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Gongronema latifolium (GL) is reported to have hepato-protective and nephron-protective ability. Diabetes is associated with liver and kidney damage. This work was designed to evaluate the effect of ethanolic leaf extract of Gongronema latifolium on liver and kidney functions in Streptozotocin-induced diabetic rats. 40 Wistar rats of both sexes (150g-200g) were divided into five (n=8). Group 1- normal control (received normal saline placebo orally). Group 2 received 200mg/kg GL orally. Group 3 was the diabetic group administered 65mg/kg of streptozotocin (STZ) intraperitoneally for two consecutive days with an interval of one day in between. Group 4 (diabetic + GL group) had STZ (65mg/kg i.p.) + oral administration of 200mg/kg GL. Group 5 was the diabetic group treated with insulin subcutaneously. Blood samples were collected via cardiac puncture to assess Liver enzymes activity, Serum electrolytes biomarkers, Urea and Creatinine. The Result shows a significant (P<0.05) increase in the glucose level of the diabetic group compared to the control. Intervention with GL and insulin significantly reduced the glucose level towards normal. The liver enzymes activity was significantly (p<0.05) increased in the diabetic group but was significantly (p<0.01) decreased following treatment with GL and insulin. Sodium, potassium, chloride, urea and creatinine were significantly (p<0.01) increased in the diabetic group with a decrease in bicarbonate. GL and insulin significantly (p<0.01) reversed these changes in electrolytes and liver enzymes activity towards normal. Results presented shows that GL just like insulin possess hypoglycemic, nephro and hepato-protective potential. Thus, in diabetic condition, Gongronema latifolium leaf extract may exert ameliorative effect on these target organs. Keywords: Gongronema Latifolium, Diabetes, insulin, serum electrolytes, liver enzymes, rats.

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Protective Effects of Bi-Herbal Formulation of Aqueous Extracts of Vernonia amygdalina and Gongronema latifolium against Gentamicin Induced Nephrotoxicity and Liver Injury in Rats

Cited by 4 publications

References 18 publications

Secondary metabolites profiling of Vernonia amygdalina Del. in response to copper-induced abiotic stress

Secondary metabolites profiling of Vernonia amygdalina Del. in response to copper-induced abiotic stress

Biochemical and Hepatoprotective Effects of Gangronema latifolium Extract on Alloxan-Induced Diabetic Rats

Hepato and Nephro-protective Potentials of Gongronema latifolium in Streptozotocin Induced diabetic Rat Model

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