Protective effects of black currant (Ribes nigrum L.) extract on hydrogen peroxide-induced damage in lung fibroblast MRC-5 cells in relation to the antioxidant activity

Identification of phenolics in litchi and evaluation of anticancer cell proliferation activity and intracellular antioxidant activity

“…Nuclear magnetic resonance ( 1 H NMR (400MHz) and 13 C NMR (100MHz)) spectra were recorded on a Bruker DRX-400 instrument (Bruker, Rheinstetten, 6 Germany). CD 3 …”

“…The cells were collected and treated with cell lysis buffer (20 mM Tris at pH 7.5, 150 mM NaCl, 1% Triton X-100) at 4 o C. The lysed cells were used to determine the intracellular activities of SOD, GSH-Px, and CAT. They were determined by kits according to the manufacturer instructions [13]. Cells without cinnamtannin B1 and ABAP treatment was used as positive control (PC), while cells 11 treated without cinnamtannin B1 but having ABAP was used as negative control (NC).…”

“…The apoptotic cells were quantified by Annexin V-FITC/PI double staining assay with Annexin V-FITC apoptosis detection kit [13,15]. As described above, HepG 2 cells were cultured at a density of 5×10 5 cells/well in six-well plates and grown for 24 h, and then the cells were exposed to different concentrations of cinnamtannin B1 (0, 50, 100, 150, 200 and 250 µM) for 24 h. After treatment, the detached cells in the medium were firstly collected, and the adhering cells were harvested by trypsinisation 14 (0.25% trypsin-EDTA, Sigma Alcrich) were collected as well.…”

“…The inhibitory activity of cinnamtannin B1 on ABAP-induced ROS12 overexpression in HepG 2 cells. The cells were pretreated with different concentrations13 …”

SCREENING OF ANTIOXIDANTS, ANTI-AGING AND TYROSINASE INHIBITORY ACTIVITIES OF ETHANOLIC AND ETHYL ACETATE EXTRACTS OF FRUIT FLESH AND FRUIT PEEL LANGSAT (Lansium domesticum Corr) IN VITRO

“…Secara normal, ROS dapat dihilangkan oleh adanya antioksidan endogen seperti superoksida dismutase (SOD), katalase (CAT), glutathione peroxidase (GPx ) dan glutathione reduktase (GR). Akan tetapi oksidatif stres terjadi ketika keseimbangan antara oksidan dan antioksidan dalam tubuh berubah oleh karena peningkatan ROS dan penurunan antioksidan dari dalam tubuh maka akan dapat menyebabkan kerusakan sel dan juga akan mempengaruhi penuaan pada kulit (Jia et al, 2014;Kim et al, 2016).…”